Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep
FAT atypical cadherin 1 (FAT1) is a member of the atypical cadherin superfamily and is involved in the planar cell polarity signalling pathway which regulates hair follicle morphogenesis, cycling, and orientation. The purpose of this study was to investigate the sheep FAT1 gene expression, polymorph...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-01-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202001-0004_polymorphisms-and-association-of-fat1-gene-with-wool-quality-traits-in-chinese-merino-sheep.php |
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| author | Guang-Wei Ma Xin You Hua Yang Xiao-Hong Yan Fang Mou Yan-Kai Chu En-Guang Rong Shou-Zhi Wang Zhi-Peng Wang Hui Li Ning Wang |
| author_facet | Guang-Wei Ma Xin You Hua Yang Xiao-Hong Yan Fang Mou Yan-Kai Chu En-Guang Rong Shou-Zhi Wang Zhi-Peng Wang Hui Li Ning Wang |
| author_sort | Guang-Wei Ma |
| collection | DOAJ |
| description | FAT atypical cadherin 1 (FAT1) is a member of the atypical cadherin superfamily and is involved in the planar cell polarity signalling pathway which regulates hair follicle morphogenesis, cycling, and orientation. The purpose of this study was to investigate the sheep FAT1 gene expression, polymorphisms and its association with wool quality traits in Chinese Merino sheep. Quantitative real-time PCR analysis showed that the FAT1 mRNA was differentially expressed in the adult skin of Chinese Merino and Kazak sheep. Seven SNPs (termed SNPs 1-7) were identified in exon 2 of sheep FAT1 gene by Sanger sequencing. SNPs 2 and 7 (novel SNPs) were significantly associated with wool crimp (P < 0.05). SNPs 4 and 5 (rs161528993 and rs161528992) were significantly associated with wool fibre length (P < 0.05). SNP 7 was highly significantly associated with average wool fibre diameter (P < 0.01). Similarly, FAT1 haplotypes were significantly associated with wool crimp (P < 0.05), and the haplotypes H1-H3 and H5 were significantly associated with higher wool crimp (P < 0.05). Bioinformatics analysis showed that the wool quality trait-associated SNPs (SNPs 2, 4, 5 and 7) might affect the pre-mRNA splicing by creating or abolishing the binding sites for serine/arginine-rich proteins, and in addition, SNPs 2 and 4 might alter the FAT1 mRNA secondary structure. Our results suggest that FAT1 influences wool quality traits and its SNPs 2, 4, 5 and 7 might be useful markers for marker-assisted selection and sheep breeding. |
| first_indexed | 2024-04-10T08:25:03Z |
| format | Article |
| id | doaj.art-64ab95bdb3a44848be554ce2154eb2d6 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:03Z |
| publishDate | 2020-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-64ab95bdb3a44848be554ce2154eb2d62023-02-23T03:33:44ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092020-01-01651313910.17221/224/2019-CJAScjs-202001-0004Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheepGuang-Wei Ma0Xin You1Hua Yang2Xiao-Hong Yan3Fang Mou4Yan-Kai Chu5En-Guang Rong6Shou-Zhi Wang7Zhi-Peng Wang8Hui Li9Ning Wang10College of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaInstitute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaState Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, P.R. ChinaFAT atypical cadherin 1 (FAT1) is a member of the atypical cadherin superfamily and is involved in the planar cell polarity signalling pathway which regulates hair follicle morphogenesis, cycling, and orientation. The purpose of this study was to investigate the sheep FAT1 gene expression, polymorphisms and its association with wool quality traits in Chinese Merino sheep. Quantitative real-time PCR analysis showed that the FAT1 mRNA was differentially expressed in the adult skin of Chinese Merino and Kazak sheep. Seven SNPs (termed SNPs 1-7) were identified in exon 2 of sheep FAT1 gene by Sanger sequencing. SNPs 2 and 7 (novel SNPs) were significantly associated with wool crimp (P < 0.05). SNPs 4 and 5 (rs161528993 and rs161528992) were significantly associated with wool fibre length (P < 0.05). SNP 7 was highly significantly associated with average wool fibre diameter (P < 0.01). Similarly, FAT1 haplotypes were significantly associated with wool crimp (P < 0.05), and the haplotypes H1-H3 and H5 were significantly associated with higher wool crimp (P < 0.05). Bioinformatics analysis showed that the wool quality trait-associated SNPs (SNPs 2, 4, 5 and 7) might affect the pre-mRNA splicing by creating or abolishing the binding sites for serine/arginine-rich proteins, and in addition, SNPs 2 and 4 might alter the FAT1 mRNA secondary structure. Our results suggest that FAT1 influences wool quality traits and its SNPs 2, 4, 5 and 7 might be useful markers for marker-assisted selection and sheep breeding.https://cjas.agriculturejournals.cz/artkey/cjs-202001-0004_polymorphisms-and-association-of-fat1-gene-with-wool-quality-traits-in-chinese-merino-sheep.phpfat atypical cadherin 1tissue expressionsnpwool crimpwool fibre diameter |
| spellingShingle | Guang-Wei Ma Xin You Hua Yang Xiao-Hong Yan Fang Mou Yan-Kai Chu En-Guang Rong Shou-Zhi Wang Zhi-Peng Wang Hui Li Ning Wang Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep Czech Journal of Animal Science fat atypical cadherin 1 tissue expression snp wool crimp wool fibre diameter |
| title | Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep |
| title_full | Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep |
| title_fullStr | Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep |
| title_full_unstemmed | Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep |
| title_short | Polymorphisms and association of FAT1 gene with wool quality traits in Chinese Merino sheep |
| title_sort | polymorphisms and association of fat1 gene with wool quality traits in chinese merino sheep |
| topic | fat atypical cadherin 1 tissue expression snp wool crimp wool fibre diameter |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-202001-0004_polymorphisms-and-association-of-fat1-gene-with-wool-quality-traits-in-chinese-merino-sheep.php |
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