Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response
Silkworm pupa (bombyx mori) protein (SPP) is a potential therapeutic bioactive substance that has anti-tumor activity against breast, liver, and gastric cancers. The aim of this study was to investigate the antitumor effect of SPP on colon cancer nude mice. Using a subcutaneous tumor formation metho...
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| Format: | Article |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1138742/full |
| _version_ | 1797777414806306816 |
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| author | Yaxi Zhou Yaxi Zhou Xiaojiao Ji Diandian Wang Yu Guo Jian Zhao Wenjie Yan Wenjie Yan |
| author_facet | Yaxi Zhou Yaxi Zhou Xiaojiao Ji Diandian Wang Yu Guo Jian Zhao Wenjie Yan Wenjie Yan |
| author_sort | Yaxi Zhou |
| collection | DOAJ |
| description | Silkworm pupa (bombyx mori) protein (SPP) is a potential therapeutic bioactive substance that has anti-tumor activity against breast, liver, and gastric cancers. The aim of this study was to investigate the antitumor effect of SPP on colon cancer nude mice. Using a subcutaneous tumor formation method, we validated the therapeutic effect of SPP on colon cancer nude mice in vivo. Results showed that SPP was cytotoxic to tumor cells. SPP could protect the liver of the nude mice by lowering hepatic oxidative stress and regulating serum inflammation levels by decreasing TNF-α and IL-2 levels while in-creasing INF-γ levels. In addition, diminished Ki-67 protein, enhanced cleaved caspase-3 protein, di-minished Vimentin, enhanced E-cadherin. These findings suggested that SPP’s antitumor activity may be achieved by reducing inflammation, inhibiting tumor proliferation and metastasis, and inducing apoptosis in cancer cells. In the future, SPP could be used as an anticancer drug, potentially providing a new source of drugs for the treatment of colon cancer. |
| first_indexed | 2024-03-12T23:04:39Z |
| format | Article |
| id | doaj.art-64abb1ef47174411b6696e6888f8cdfc |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-12T23:04:39Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-64abb1ef47174411b6696e6888f8cdfc2023-07-19T05:07:30ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-07-011410.3389/fphar.2023.11387421138742Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory responseYaxi Zhou0Yaxi Zhou1Xiaojiao Ji2Diandian Wang3Yu Guo4Jian Zhao5Wenjie Yan6Wenjie Yan7College of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaCollege of Biochemical Engineering, Beijing Union University, Beijing, ChinaBeijing Key Laboratory of Bioactive Substances and Functional Food, College of Biochemical Engineering, Beijing Union University, Beijing, ChinaSilkworm pupa (bombyx mori) protein (SPP) is a potential therapeutic bioactive substance that has anti-tumor activity against breast, liver, and gastric cancers. The aim of this study was to investigate the antitumor effect of SPP on colon cancer nude mice. Using a subcutaneous tumor formation method, we validated the therapeutic effect of SPP on colon cancer nude mice in vivo. Results showed that SPP was cytotoxic to tumor cells. SPP could protect the liver of the nude mice by lowering hepatic oxidative stress and regulating serum inflammation levels by decreasing TNF-α and IL-2 levels while in-creasing INF-γ levels. In addition, diminished Ki-67 protein, enhanced cleaved caspase-3 protein, di-minished Vimentin, enhanced E-cadherin. These findings suggested that SPP’s antitumor activity may be achieved by reducing inflammation, inhibiting tumor proliferation and metastasis, and inducing apoptosis in cancer cells. In the future, SPP could be used as an anticancer drug, potentially providing a new source of drugs for the treatment of colon cancer.https://www.frontiersin.org/articles/10.3389/fphar.2023.1138742/fullBombyx morisilkworm pupae proteincolon canceranti-tumorinflammatory responseshealth benefit |
| spellingShingle | Yaxi Zhou Yaxi Zhou Xiaojiao Ji Diandian Wang Yu Guo Jian Zhao Wenjie Yan Wenjie Yan Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response Frontiers in Pharmacology Bombyx mori silkworm pupae protein colon cancer anti-tumor inflammatory responses health benefit |
| title | Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response |
| title_full | Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response |
| title_fullStr | Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response |
| title_full_unstemmed | Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response |
| title_short | Effect of silkworm pupae (Bombyx mori) protein on colon cancer in nude mice: inhibition of tumor growth, oxidative stress and inflammatory response |
| title_sort | effect of silkworm pupae bombyx mori protein on colon cancer in nude mice inhibition of tumor growth oxidative stress and inflammatory response |
| topic | Bombyx mori silkworm pupae protein colon cancer anti-tumor inflammatory responses health benefit |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1138742/full |
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