Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing
Natural hydrogels are growing in interest as a priority for wound healing. Plant polysaccharides have a variety of biological pharmacological activities, and chitosan hydrogels have proven strong antimicrobial effects, but hydrogels prepared with polysaccharides alone have certain deficiencies. Poly...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/9/1716 |
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| author | Hongyan Xue Meng Sun Xiaoliang Zhao Yonggang Wang Jinxin Yan Weijie Zhang |
| author_facet | Hongyan Xue Meng Sun Xiaoliang Zhao Yonggang Wang Jinxin Yan Weijie Zhang |
| author_sort | Hongyan Xue |
| collection | DOAJ |
| description | Natural hydrogels are growing in interest as a priority for wound healing. Plant polysaccharides have a variety of biological pharmacological activities, and chitosan hydrogels have proven strong antimicrobial effects, but hydrogels prepared with polysaccharides alone have certain deficiencies. Polysaccharides from flowers of <i>Lonicera japonica</i> Thunb. (LP) and the aerial parts of <i>Mentha canadensis</i> L. (MP) were extracted and oxidized by sodium periodate (NaIO<sub>4</sub>) and then cross-linked with oxidized-carboxymethylated chitosan (O-CCS) to develop oxidized plant- polysaccharides-chitosan hydrogels (OPHs). SEM observation showed that OPHs had porous interior structures with interconnecting pores. The OPHs showed good swelling, water-retention ability, blood coagulation, cytocompatibility properties, and low cytotoxicity (classed as grade 1 according to United States Pharmacopoeia), which met the requirements for wound dressings. Then the cutaneous wound-healing effect was evaluated in BALB/C mice model, after 7 days treatment, the wound-closure rate of OPHs groups were all greater than 50%, and after 14 days, all were greater than 90%, while the value of the control group was only 72.6%. Of them, OPH-2 and OPH-3 were more favorable to the wound-healing process, as the promotion was more significant. The plant polysaccharides and CS-based hydrogel should be a candidate for cutaneous wound dressings. |
| first_indexed | 2024-03-10T03:46:52Z |
| format | Article |
| id | doaj.art-64ac55ad257e4128a7fa0067055d12aa |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:46:52Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-64ac55ad257e4128a7fa0067055d12aa2023-11-23T09:05:13ZengMDPI AGPolymers2073-43602022-04-01149171610.3390/polym14091716Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound HealingHongyan Xue0Meng Sun1Xiaoliang Zhao2Yonggang Wang3Jinxin Yan4Weijie Zhang5School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaNatural hydrogels are growing in interest as a priority for wound healing. Plant polysaccharides have a variety of biological pharmacological activities, and chitosan hydrogels have proven strong antimicrobial effects, but hydrogels prepared with polysaccharides alone have certain deficiencies. Polysaccharides from flowers of <i>Lonicera japonica</i> Thunb. (LP) and the aerial parts of <i>Mentha canadensis</i> L. (MP) were extracted and oxidized by sodium periodate (NaIO<sub>4</sub>) and then cross-linked with oxidized-carboxymethylated chitosan (O-CCS) to develop oxidized plant- polysaccharides-chitosan hydrogels (OPHs). SEM observation showed that OPHs had porous interior structures with interconnecting pores. The OPHs showed good swelling, water-retention ability, blood coagulation, cytocompatibility properties, and low cytotoxicity (classed as grade 1 according to United States Pharmacopoeia), which met the requirements for wound dressings. Then the cutaneous wound-healing effect was evaluated in BALB/C mice model, after 7 days treatment, the wound-closure rate of OPHs groups were all greater than 50%, and after 14 days, all were greater than 90%, while the value of the control group was only 72.6%. Of them, OPH-2 and OPH-3 were more favorable to the wound-healing process, as the promotion was more significant. The plant polysaccharides and CS-based hydrogel should be a candidate for cutaneous wound dressings.https://www.mdpi.com/2073-4360/14/9/1716hydrogelchitosan<i>Lonicera japonica</i> Thunb. polysaccharides<i>Mentha canadensis</i> L. polysaccharideswound healingskin defect model |
| spellingShingle | Hongyan Xue Meng Sun Xiaoliang Zhao Yonggang Wang Jinxin Yan Weijie Zhang Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing Polymers hydrogel chitosan <i>Lonicera japonica</i> Thunb. polysaccharides <i>Mentha canadensis</i> L. polysaccharides wound healing skin defect model |
| title | Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing |
| title_full | Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing |
| title_fullStr | Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing |
| title_full_unstemmed | Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing |
| title_short | Preparation and Characterization of Polysaccharide-Based Hydrogels for Cutaneous Wound Healing |
| title_sort | preparation and characterization of polysaccharide based hydrogels for cutaneous wound healing |
| topic | hydrogel chitosan <i>Lonicera japonica</i> Thunb. polysaccharides <i>Mentha canadensis</i> L. polysaccharides wound healing skin defect model |
| url | https://www.mdpi.com/2073-4360/14/9/1716 |
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