Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy
The toxicological implications of nanoparticles deserve accurate scientific investigation for the protection of human health. Although toxic effects involve specific organs, the events that cause them have their origin from biochemical modifications of some cellular constituents. Therefore, a first...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/10/2418 |
| _version_ | 1811263692067569664 |
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| author | Maria Lasalvia Giuseppe Perna Vito Capozzi |
| author_facet | Maria Lasalvia Giuseppe Perna Vito Capozzi |
| author_sort | Maria Lasalvia |
| collection | DOAJ |
| description | The toxicological implications of nanoparticles deserve accurate scientific investigation for the protection of human health. Although toxic effects involve specific organs, the events that cause them have their origin from biochemical modifications of some cellular constituents. Therefore, a first analysis to evaluate the effects due to the action of nanoparticles is achieved by investigation of in vitro cells, which allows the identification of the cellular modifications caused by nanoparticles (NPs) even at much lower doses than the lethal ones. This work evaluated the Raman microspectroscopy capability to monitor biochemical changes occurring in human cells as a consequence of exposure to a suspension of gold nanoparticles with a non-cytotoxic concentration. Human keratinocyte cells were used as a model cell line, because they are mainly involved in environmental exposure. A trypan blue assay revealed that the investigated concentration, 650 ng/mL, is non-cytotoxic (about 5% of cells died after 48 h exposure). Specific Raman spectral markers to represent the cell response to nanoparticle exposure were found (at 1450 and 2865 cm<sup>−1</sup>) in the cytoplasm spectra, with the aid of ratiometric and principal component analysis. |
| first_indexed | 2024-04-12T19:49:51Z |
| format | Article |
| id | doaj.art-64ac83028677491cb0e06bed8eac313c |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T19:49:51Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-64ac83028677491cb0e06bed8eac313c2022-12-22T03:18:52ZengMDPI AGSensors1424-82202019-05-011910241810.3390/s19102418s19102418Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman MicrospectroscopyMaria Lasalvia0Giuseppe Perna1Vito Capozzi2Dipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyDipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyDipartimento di Medicina Clinica e Sperimentale, Università di Foggia, 71122 Foggia, ItalyThe toxicological implications of nanoparticles deserve accurate scientific investigation for the protection of human health. Although toxic effects involve specific organs, the events that cause them have their origin from biochemical modifications of some cellular constituents. Therefore, a first analysis to evaluate the effects due to the action of nanoparticles is achieved by investigation of in vitro cells, which allows the identification of the cellular modifications caused by nanoparticles (NPs) even at much lower doses than the lethal ones. This work evaluated the Raman microspectroscopy capability to monitor biochemical changes occurring in human cells as a consequence of exposure to a suspension of gold nanoparticles with a non-cytotoxic concentration. Human keratinocyte cells were used as a model cell line, because they are mainly involved in environmental exposure. A trypan blue assay revealed that the investigated concentration, 650 ng/mL, is non-cytotoxic (about 5% of cells died after 48 h exposure). Specific Raman spectral markers to represent the cell response to nanoparticle exposure were found (at 1450 and 2865 cm<sup>−1</sup>) in the cytoplasm spectra, with the aid of ratiometric and principal component analysis.https://www.mdpi.com/1424-8220/19/10/2418Raman microspectroscopyhuman cellsnanotoxicology |
| spellingShingle | Maria Lasalvia Giuseppe Perna Vito Capozzi Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy Sensors Raman microspectroscopy human cells nanotoxicology |
| title | Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy |
| title_full | Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy |
| title_fullStr | Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy |
| title_full_unstemmed | Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy |
| title_short | Biochemical Changes in Human Cells Exposed to Low Concentrations of Gold Nanoparticles Detected by Raman Microspectroscopy |
| title_sort | biochemical changes in human cells exposed to low concentrations of gold nanoparticles detected by raman microspectroscopy |
| topic | Raman microspectroscopy human cells nanotoxicology |
| url | https://www.mdpi.com/1424-8220/19/10/2418 |
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