Macromolecular protein crystallisation with biotemplate of live cells
Abstract Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the crystallisation processes of lysozyme, wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-06999-7 |
| _version_ | 1818501260816416768 |
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| author | Mubai Sun Huaiyu Yang Xinyu Miao Weixian Wang Jinghui Wang |
| author_facet | Mubai Sun Huaiyu Yang Xinyu Miao Weixian Wang Jinghui Wang |
| author_sort | Mubai Sun |
| collection | DOAJ |
| description | Abstract Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the crystallisation processes of lysozyme, with different concentrations from 20 to 2.5 mg/mL lysozyme and different concentrations from 0 to 5.0 × 107 (cfu/mL) Saccharomyces cerevisiae cells, during a period of 96 h. During the crystallisation period, the nucleation possibility in droplets, crystal numbers, and cell growth and cell density were observed and analysed. The results indicated the strong interaction between the lysozyme molecules and the cell wall of the S. cerevisiae, proved by the crystallization of lysozyme with fluorescent labels. The biotemplates demonstrated positive influence or negative influence on the nucleation, i.e. shorter or longer induction time, dependent on the concentrations of the lysozyme and the S. cerevisiae cells, and ratios between them. In the biomanufacturing process, target proteins were various cells were commonly mixed with various cells, and this work provides novel insights of new design and application of live cells as biotemplates for purification of macromolecules. |
| first_indexed | 2024-12-10T20:53:45Z |
| format | Article |
| id | doaj.art-64ad13db0a664d938220d1494b649e20 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T20:53:45Z |
| publishDate | 2022-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-64ad13db0a664d938220d1494b649e202022-12-22T01:34:01ZengNature PortfolioScientific Reports2045-23222022-02-0112111010.1038/s41598-022-06999-7Macromolecular protein crystallisation with biotemplate of live cellsMubai Sun0Huaiyu Yang1Xinyu Miao2Weixian Wang3Jinghui Wang4Agricultural Products Processing Research Institute, Jilin Academy of Agricultural ScienceDepartment of Chemical Engineering, Loughborough UniversityAgricultural Products Processing Research Institute, Jilin Academy of Agricultural ScienceSchool of Materials and Chemistry, University of Shanghai for Science and TechnologyAgricultural Products Processing Research Institute, Jilin Academy of Agricultural ScienceAbstract Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the crystallisation processes of lysozyme, with different concentrations from 20 to 2.5 mg/mL lysozyme and different concentrations from 0 to 5.0 × 107 (cfu/mL) Saccharomyces cerevisiae cells, during a period of 96 h. During the crystallisation period, the nucleation possibility in droplets, crystal numbers, and cell growth and cell density were observed and analysed. The results indicated the strong interaction between the lysozyme molecules and the cell wall of the S. cerevisiae, proved by the crystallization of lysozyme with fluorescent labels. The biotemplates demonstrated positive influence or negative influence on the nucleation, i.e. shorter or longer induction time, dependent on the concentrations of the lysozyme and the S. cerevisiae cells, and ratios between them. In the biomanufacturing process, target proteins were various cells were commonly mixed with various cells, and this work provides novel insights of new design and application of live cells as biotemplates for purification of macromolecules.https://doi.org/10.1038/s41598-022-06999-7 |
| spellingShingle | Mubai Sun Huaiyu Yang Xinyu Miao Weixian Wang Jinghui Wang Macromolecular protein crystallisation with biotemplate of live cells Scientific Reports |
| title | Macromolecular protein crystallisation with biotemplate of live cells |
| title_full | Macromolecular protein crystallisation with biotemplate of live cells |
| title_fullStr | Macromolecular protein crystallisation with biotemplate of live cells |
| title_full_unstemmed | Macromolecular protein crystallisation with biotemplate of live cells |
| title_short | Macromolecular protein crystallisation with biotemplate of live cells |
| title_sort | macromolecular protein crystallisation with biotemplate of live cells |
| url | https://doi.org/10.1038/s41598-022-06999-7 |
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