Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon
The temperate wild grass Brachypodium distachyon (Brachypodium) serves as model system for studying turf and forage grasses. Brachypodium collections show diverse responses to drought stress, but little is known about the genetic mechanisms of drought tolerance of this species. The objective of this...
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.00811/full |
| _version_ | 1811270056002191360 |
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| author | Yiwei Jiang Yiwei Jiang Xicheng Wang Xiaoqing Yu Xiongwei Zhao Xiongwei Zhao Na Luo Zhongyou Pei Huifen Liu David F. Garvin David F. Garvin |
| author_facet | Yiwei Jiang Yiwei Jiang Xicheng Wang Xiaoqing Yu Xiongwei Zhao Xiongwei Zhao Na Luo Zhongyou Pei Huifen Liu David F. Garvin David F. Garvin |
| author_sort | Yiwei Jiang |
| collection | DOAJ |
| description | The temperate wild grass Brachypodium distachyon (Brachypodium) serves as model system for studying turf and forage grasses. Brachypodium collections show diverse responses to drought stress, but little is known about the genetic mechanisms of drought tolerance of this species. The objective of this study was to identify quantitative trait loci (QTLs) associated with drought tolerance traits in Brachypodium. We assessed leaf fresh weight (LFW), leaf dry weight (LDW), leaf water content (LWC), leaf wilting (WT), and chlorophyll fluorescence (Fv/Fm) under well-watered and drought conditions on a recombinant inbred line (RIL) population from two parents (Bd3-1 and Bd1-1) known to differ in their drought adaptation. A linkage map of the RIL population was constructed using 467 single nucleotide polymorphism (SNP) markers obtained from genotyping-by-sequencing. The Bd3-1/Bd1-1 map spanned 1,618 cM and had an average distance of 3.5 cM between adjacent single nucleotide polymorphisms (SNPs). Twenty-six QTLs were identified in chromosome 1, 2, and 3 in two experiments, with 14 of the QTLs under well-watered conditions and 12 QTLs under drought stress. In Experiment 1, a QTL located on chromosome 2 with a peak at 182 cM appeared to simultaneously control WT, LWC, and Fv/Fm under drought stress, accounting for 11–18.7% of the phenotypic variation. Allelic diversity of candidate genes DREB2B, MYB, and SPK, which reside in one multi-QTL region, may play a role in the natural variation in whole plant drought tolerance in Brachypodium. Co-localization of QTLs for multiple drought-related traits suggest that the gene(s) involved are important regulators of drought tolerance in Brachypodium. |
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| format | Article |
| id | doaj.art-64ae900bd5384c0db486a3de1dc963d9 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T21:54:05Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-64ae900bd5384c0db486a3de1dc963d92022-12-22T03:15:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00811264511Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyonYiwei Jiang0Yiwei Jiang1Xicheng Wang2Xiaoqing Yu3Xiongwei Zhao4Xiongwei Zhao5Na Luo6Zhongyou Pei7Huifen Liu8David F. Garvin9David F. Garvin10College of Agronomy, Resources, and Environment, Tianjin Agricultural UniversityTianjin, ChinaDepartment of Agronomy, Purdue UniversityWest Lafayette, IN, United StatesInstitute of Horticulture, Jiangsu Academy of Agricultural SciencesNanjing, ChinaDepartment of Agronomy, Iowa State UniversityAmes, IA, United StatesDepartment of Agronomy, Purdue UniversityWest Lafayette, IN, United StatesDepartment of Crop Genetics and Breeding, Sichuan Agricultural UniversityChengdu, ChinaCollege of Life Sciences, South China Agricultural UniversityGuangzhou, ChinaCollege of Agronomy, Resources, and Environment, Tianjin Agricultural UniversityTianjin, ChinaCollege of Agronomy, Resources, and Environment, Tianjin Agricultural UniversityTianjin, ChinaDepartment of Agronomy and Plant Genetics, University of MinnesotaSt. Paul, MN, United StatesPlant Science Research Unit, United States Department of Agriculture, Agricultural Research ServiceSt. Paul, MN, United StatesThe temperate wild grass Brachypodium distachyon (Brachypodium) serves as model system for studying turf and forage grasses. Brachypodium collections show diverse responses to drought stress, but little is known about the genetic mechanisms of drought tolerance of this species. The objective of this study was to identify quantitative trait loci (QTLs) associated with drought tolerance traits in Brachypodium. We assessed leaf fresh weight (LFW), leaf dry weight (LDW), leaf water content (LWC), leaf wilting (WT), and chlorophyll fluorescence (Fv/Fm) under well-watered and drought conditions on a recombinant inbred line (RIL) population from two parents (Bd3-1 and Bd1-1) known to differ in their drought adaptation. A linkage map of the RIL population was constructed using 467 single nucleotide polymorphism (SNP) markers obtained from genotyping-by-sequencing. The Bd3-1/Bd1-1 map spanned 1,618 cM and had an average distance of 3.5 cM between adjacent single nucleotide polymorphisms (SNPs). Twenty-six QTLs were identified in chromosome 1, 2, and 3 in two experiments, with 14 of the QTLs under well-watered conditions and 12 QTLs under drought stress. In Experiment 1, a QTL located on chromosome 2 with a peak at 182 cM appeared to simultaneously control WT, LWC, and Fv/Fm under drought stress, accounting for 11–18.7% of the phenotypic variation. Allelic diversity of candidate genes DREB2B, MYB, and SPK, which reside in one multi-QTL region, may play a role in the natural variation in whole plant drought tolerance in Brachypodium. Co-localization of QTLs for multiple drought-related traits suggest that the gene(s) involved are important regulators of drought tolerance in Brachypodium.http://journal.frontiersin.org/article/10.3389/fpls.2017.00811/fullallelic variationBrachypodium distachyondrought tolerancegenetic mapQTL mappingSNP marker |
| spellingShingle | Yiwei Jiang Yiwei Jiang Xicheng Wang Xiaoqing Yu Xiongwei Zhao Xiongwei Zhao Na Luo Zhongyou Pei Huifen Liu David F. Garvin David F. Garvin Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon Frontiers in Plant Science allelic variation Brachypodium distachyon drought tolerance genetic map QTL mapping SNP marker |
| title | Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon |
| title_full | Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon |
| title_fullStr | Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon |
| title_full_unstemmed | Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon |
| title_short | Quantitative Trait Loci Associated with Drought Tolerance in Brachypodium distachyon |
| title_sort | quantitative trait loci associated with drought tolerance in brachypodium distachyon |
| topic | allelic variation Brachypodium distachyon drought tolerance genetic map QTL mapping SNP marker |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.00811/full |
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