Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis

Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions from hamste...

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Main Authors: A Lugo de Yarbuh, C Colasante, M Alarcón, E Moreno
Format: Article
Language:English
Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2004-11-01
Series:Kasmera
Subjects:
Online Access:https://produccioncientificaluz.org/index.php/kasmera/article/view/4744
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author A Lugo de Yarbuh
C Colasante
M Alarcón
E Moreno
author_facet A Lugo de Yarbuh
C Colasante
M Alarcón
E Moreno
author_sort A Lugo de Yarbuh
collection DOAJ
description Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions from hamsters infected with the L. garnhami were pressed on amicroscope slide, allowed to dry and methanol-fixed in order to be ultrastructurally analyzed using atomic force microscopy (AFM). Free amastigotes showed their nucleus, kinetoplast and a depression of 5microns, corresponding to the refractil organelle characteristic of this Leishmania. Parasites inside macrophages and lymphocytes and the topographical relationship with the host cytoplasm was also observed. Parasite-host-cell interaction revealed different membrane contact. The amastigote-macrophage contact is established by small macrophage filopodio associated with its cytoplasmic reduction near the contact site of 800 nm. The amastigote-lymphocyte contact shows fusion-like behavior between the two membranes without any cell specialization. The cutaneous lesion studied with AFM allowed observation with high resolution the close contact established between the parasite and its host cells as well as details of the fine structure of the amastigotes, applying a simple and rapid tissue preparation.
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spelling doaj.art-64ae9d24d9f44b82857f4b20e5dad8a52023-09-03T10:04:59ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282004-11-013221271364743Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasisA Lugo de Yarbuh0C Colasante1M Alarcón2E Moreno3Laboratorio de Parasitología Experimental, Departamento de Biología, Facultad de Ciencias, Universidad de los Andes.Laboratorio de Fisiología de la Conducta, Facultad de Medicina Universidad de los Andes.Laboratorio de Parasitología Experimental, Departamento de Biología, Facultad de Ciencias, Universidad de los Andes.Laboratorio de Parasitología Experimental, Departamento de Biología, Facultad de Ciencias, Universidad de los Andes.Natural infection produced by the entry of promastigotes into the skin cells induces cutaneous leishmaniasis disease and the Interaction between phagocytic cells and different strains of Leishmania determine the course of disease in the mammalian host. In this study the cutaneous lesions from hamsters infected with the L. garnhami were pressed on amicroscope slide, allowed to dry and methanol-fixed in order to be ultrastructurally analyzed using atomic force microscopy (AFM). Free amastigotes showed their nucleus, kinetoplast and a depression of 5microns, corresponding to the refractil organelle characteristic of this Leishmania. Parasites inside macrophages and lymphocytes and the topographical relationship with the host cytoplasm was also observed. Parasite-host-cell interaction revealed different membrane contact. The amastigote-macrophage contact is established by small macrophage filopodio associated with its cytoplasmic reduction near the contact site of 800 nm. The amastigote-lymphocyte contact shows fusion-like behavior between the two membranes without any cell specialization. The cutaneous lesion studied with AFM allowed observation with high resolution the close contact established between the parasite and its host cells as well as details of the fine structure of the amastigotes, applying a simple and rapid tissue preparation.https://produccioncientificaluz.org/index.php/kasmera/article/view/4744Microscopía de fuerza atómica, Leishmania garnhami, lesiones cutáneas, amastigotes, macrófagos, linfocitos, imagen 3D
spellingShingle A Lugo de Yarbuh
C Colasante
M Alarcón
E Moreno
Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
Kasmera
Microscopía de fuerza atómica, Leishmania garnhami, lesiones cutáneas, amastigotes, macrófagos, linfocitos, imagen 3D
title Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
title_full Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
title_fullStr Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
title_full_unstemmed Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
title_short Atomic Force Microscopy of host cell-amastigote interaction in cutaneous leishmaniasis
title_sort atomic force microscopy of host cell amastigote interaction in cutaneous leishmaniasis
topic Microscopía de fuerza atómica, Leishmania garnhami, lesiones cutáneas, amastigotes, macrófagos, linfocitos, imagen 3D
url https://produccioncientificaluz.org/index.php/kasmera/article/view/4744
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AT ccolasante atomicforcemicroscopyofhostcellamastigoteinteractionincutaneousleishmaniasis
AT malarcon atomicforcemicroscopyofhostcellamastigoteinteractionincutaneousleishmaniasis
AT emoreno atomicforcemicroscopyofhostcellamastigoteinteractionincutaneousleishmaniasis