Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach
The present study was aimed to evaluate the in vivo radioprotective efficacy of quercetin-3-O-rutinoside (PMC-1), the key bioactive constituent flavonoid glycoside isolated from the whole plant of Pilea microphylla was evaluated. In vivo survival studies established the optimum effective dose of PMC...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464612001053 |
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| author | Punit Bansal Piya Paul Amit Kunwar S. Jayakumar Pawan G. Nayak K.I. Priyadarsini M.K. Unnikrishnan |
| author_facet | Punit Bansal Piya Paul Amit Kunwar S. Jayakumar Pawan G. Nayak K.I. Priyadarsini M.K. Unnikrishnan |
| author_sort | Punit Bansal |
| collection | DOAJ |
| description | The present study was aimed to evaluate the in vivo radioprotective efficacy of quercetin-3-O-rutinoside (PMC-1), the key bioactive constituent flavonoid glycoside isolated from the whole plant of Pilea microphylla was evaluated. In vivo survival studies established the optimum effective dose of PMC-1 at 25 mg/kg/i.p. At the optimum dose, PMC-1 prevented the depletion of endogenous antioxidants in the liver of irradiated mice. In vivo protection towards gastrointestinal tract and haematopoietic system was confirmed by the restoration of radiation-induced reduction in villi height, number of crypt cells and spleen index. PMC-1 also attenuated the radiation-induced apoptosis in spleenocytes significantly. Single cell gel electrophoresis of peripheral blood leukocytes showed inhibition of radiation-induced DNA damage by PMC-1. PMC-1 pretreatment significantly reversed the changes by increasing pro-survival (ERK) and decreasing pro-apoptotic (BAX) gene expressions compared to radiation control. Thus, PMC-1 exhibits protective effects against γ-radiation and the probable mechanism of action involves maintenance of antioxidant enzymes, prophylactic action and inhibition of apoptosis. |
| first_indexed | 2024-12-14T10:41:45Z |
| format | Article |
| id | doaj.art-64b0e47dfb1e4ab5b1523e3e96d841ab |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:41:45Z |
| publishDate | 2012-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-64b0e47dfb1e4ab5b1523e3e96d841ab2022-12-21T23:05:39ZengElsevierJournal of Functional Foods1756-46462012-10-0144924932Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approachPunit Bansal0Piya Paul1Amit Kunwar2S. Jayakumar3Pawan G. Nayak4K.I. Priyadarsini5M.K. Unnikrishnan6Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, IndiaRadiation & Photochemistry Division, Bhabha Atomic Research Center, Mumbai 400 085, IndiaRadiation Biology & Health Sciences Division, Bhabha Atomic Research Center, Mumbai 400 085, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, IndiaRadiation & Photochemistry Division, Bhabha Atomic Research Center, Mumbai 400 085, IndiaDepartment of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104, Karnataka, India; Corresponding author. Tel.: +91 820 2922454; fax: +91 820 2571998.The present study was aimed to evaluate the in vivo radioprotective efficacy of quercetin-3-O-rutinoside (PMC-1), the key bioactive constituent flavonoid glycoside isolated from the whole plant of Pilea microphylla was evaluated. In vivo survival studies established the optimum effective dose of PMC-1 at 25 mg/kg/i.p. At the optimum dose, PMC-1 prevented the depletion of endogenous antioxidants in the liver of irradiated mice. In vivo protection towards gastrointestinal tract and haematopoietic system was confirmed by the restoration of radiation-induced reduction in villi height, number of crypt cells and spleen index. PMC-1 also attenuated the radiation-induced apoptosis in spleenocytes significantly. Single cell gel electrophoresis of peripheral blood leukocytes showed inhibition of radiation-induced DNA damage by PMC-1. PMC-1 pretreatment significantly reversed the changes by increasing pro-survival (ERK) and decreasing pro-apoptotic (BAX) gene expressions compared to radiation control. Thus, PMC-1 exhibits protective effects against γ-radiation and the probable mechanism of action involves maintenance of antioxidant enzymes, prophylactic action and inhibition of apoptosis.http://www.sciencedirect.com/science/article/pii/S1756464612001053Quercetin-3-O-rutinosideFlavonoid glycosideRadioprotectionDNA damageApoptosis |
| spellingShingle | Punit Bansal Piya Paul Amit Kunwar S. Jayakumar Pawan G. Nayak K.I. Priyadarsini M.K. Unnikrishnan Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach Journal of Functional Foods Quercetin-3-O-rutinoside Flavonoid glycoside Radioprotection DNA damage Apoptosis |
| title | Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach |
| title_full | Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach |
| title_fullStr | Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach |
| title_full_unstemmed | Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach |
| title_short | Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach |
| title_sort | radioprotection by quercetin 3 o rutinoside a flavonoid glycoside a cellular and mechanistic approach |
| topic | Quercetin-3-O-rutinoside Flavonoid glycoside Radioprotection DNA damage Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464612001053 |
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