Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport
7-Ketophytosterols are the major oxidation products of phytosterols in foods, which have been associated with atherosclerosis. However, their absorption mechanism remains unclear. The aim of our work was to investigate the absorption mechanism of 7-ketophytosterols and their effects on the cholester...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2023-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000320 |
| _version_ | 1797701667899047936 |
|---|---|
| author | Mengmeng Wang Min Yu Amel Thanina Amrouche Fan Jie Shengyang Ji Baiyi Lu |
| author_facet | Mengmeng Wang Min Yu Amel Thanina Amrouche Fan Jie Shengyang Ji Baiyi Lu |
| author_sort | Mengmeng Wang |
| collection | DOAJ |
| description | 7-Ketophytosterols are the major oxidation products of phytosterols in foods, which have been associated with atherosclerosis. However, their absorption mechanism remains unclear. The aim of our work was to investigate the absorption mechanism of 7-ketophytosterols and their effects on the cholesterol transport using Caco-2 cell model. The absorption percentage of 7-ketositosterol and 7-ketocampesterol was 1.16 %−1.68 % and 1.18 %−2.23 % respectively in the Caco-2 model, which is higher than that of their parent phytosterols, but lower than cholesterol-d7. The apparent permeability of 7-ketositosterol and 7-ketocampesterol at 30 μmol/L in the basolateral (BL)-to-apical (AP) direction were 0.42- and 0.55-fold of that in the AP-to-BL direction, indicating an active intake in the permeation mechanism of 7-ketophytosterols. Ezetimibe could significantly inhibit the transport of 7-ketophytosterols (P < 0.05), which means that their transport depends on niemann-pick c1-like 1 (NPC1L1) protein. The transport of cholesterol-d7 was significantly inhibited by 7-ketophytosterols (P < 0.05). Taken together, this study deepened our understanding of the absorption mechanism of common food-born 7-ketophytosterols and provides useful information on the inhibition of 7-ketophytosterols absorption. |
| first_indexed | 2024-03-12T04:39:04Z |
| format | Article |
| id | doaj.art-64b321b6ff7d44c98b448b3d74325a3c |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T04:39:04Z |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-64b321b6ff7d44c98b448b3d74325a3c2023-09-03T09:48:12ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112517011707Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transportMengmeng Wang0Min Yu1Amel Thanina Amrouche2Fan Jie3Shengyang Ji4Baiyi Lu5National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaNational Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaNational Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaNational Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaNational Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Corresponding author at: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.7-Ketophytosterols are the major oxidation products of phytosterols in foods, which have been associated with atherosclerosis. However, their absorption mechanism remains unclear. The aim of our work was to investigate the absorption mechanism of 7-ketophytosterols and their effects on the cholesterol transport using Caco-2 cell model. The absorption percentage of 7-ketositosterol and 7-ketocampesterol was 1.16 %−1.68 % and 1.18 %−2.23 % respectively in the Caco-2 model, which is higher than that of their parent phytosterols, but lower than cholesterol-d7. The apparent permeability of 7-ketositosterol and 7-ketocampesterol at 30 μmol/L in the basolateral (BL)-to-apical (AP) direction were 0.42- and 0.55-fold of that in the AP-to-BL direction, indicating an active intake in the permeation mechanism of 7-ketophytosterols. Ezetimibe could significantly inhibit the transport of 7-ketophytosterols (P < 0.05), which means that their transport depends on niemann-pick c1-like 1 (NPC1L1) protein. The transport of cholesterol-d7 was significantly inhibited by 7-ketophytosterols (P < 0.05). Taken together, this study deepened our understanding of the absorption mechanism of common food-born 7-ketophytosterols and provides useful information on the inhibition of 7-ketophytosterols absorption.http://www.sciencedirect.com/science/article/pii/S22134530230003207-KetophytosterolsAbsorption mechanismCholesterol absorptionNiemann-pick c1-like 1 |
| spellingShingle | Mengmeng Wang Min Yu Amel Thanina Amrouche Fan Jie Shengyang Ji Baiyi Lu Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport Food Science and Human Wellness 7-Ketophytosterols Absorption mechanism Cholesterol absorption Niemann-pick c1-like 1 |
| title | Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport |
| title_full | Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport |
| title_fullStr | Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport |
| title_full_unstemmed | Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport |
| title_short | Human intestinal Caco-2 cell model to evaluate the absorption of 7-ketophytosterols and their effects on cholesterol transport |
| title_sort | human intestinal caco 2 cell model to evaluate the absorption of 7 ketophytosterols and their effects on cholesterol transport |
| topic | 7-Ketophytosterols Absorption mechanism Cholesterol absorption Niemann-pick c1-like 1 |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000320 |
| work_keys_str_mv | AT mengmengwang humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport AT minyu humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport AT amelthaninaamrouche humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport AT fanjie humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport AT shengyangji humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport AT baiyilu humanintestinalcaco2cellmodeltoevaluatetheabsorptionof7ketophytosterolsandtheireffectsoncholesteroltransport |