Evaluation of the effect of three disinfectants on removing HBV contamination
Background and Aim: Infection control is an important issue in dentistry. Without an efficient infection control, pathogens left on instruments and working surfaces will have potential danger to patients’ health. In this research, antiviral effect of three disinfectants: 0.5% sodium hypochlorite 0.0...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2006-06-01
|
| Series: | Journal of Dental Medicine |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/2518.pdf&manuscript_id=2518 |
| _version_ | 1818138785019330560 |
|---|---|
| author | Arami S Tavassoty Kheiri M Bashar R Nadali MA |
| author_facet | Arami S Tavassoty Kheiri M Bashar R Nadali MA |
| author_sort | Arami S |
| collection | DOAJ |
| description | Background and Aim: Infection control is an important issue in dentistry. Without an efficient infection control, pathogens left on instruments and working surfaces will have potential danger to patients’ health. In this research, antiviral effect of three disinfectants: 0.5% sodium hypochlorite 0.05% sodium hypochlorite and Deconex 50 AF, on HBV was investigated. Materials and Methods: In this interventional (before-after) study; serums of 26 HBV positive patients were analyzed by PCR HBV analysis and 9 contaminated species were obtained to test three disinfectants. 36 agar plates were prepared with the contaminated serums. 27 of the plates were disinfected in 3 separate groups with the above mentioned solutions. Nine remaining plates were not disinfected (control). Swabs wetted by BSAS (Bovine Serum Albumin Sodium Chloride) medium were applied on the surface of the plates and the were kept in the transferred medium and sent to virology-lab of Pasteur Institute. HBV DNA were detected by commercial kit of HBV PCR (polymerase chain reaction) method. Data were analyzed by Cochrane test with p<0.05 as the limit of significance. Results: None of samples disinfected with 0.5% sodium hypochlorite showed contamination. 11/1% of samples disinfected with 0.05% sodium hypochlorite and 44/4% of samples disinfected with Deconex 50 AF remained contaminated. Statistical analysis showed a significant difference between 0.5% sodium hypochlorite and the other groups. Conclusion: Our findings revealed that 0.5% sodium hypochlorite solution is a strong and efficient disinfectant against HBV. Key Words: HBV; Sodium hypochlorite |
| first_indexed | 2024-12-11T10:17:42Z |
| format | Article |
| id | doaj.art-64b52f52c1b4438a83b39327aac12e21 |
| institution | Directory Open Access Journal |
| issn | 1024-641X 2008-2444 |
| language | fas |
| last_indexed | 2024-12-11T10:17:42Z |
| publishDate | 2006-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Dental Medicine |
| spelling | doaj.art-64b52f52c1b4438a83b39327aac12e212022-12-22T01:11:34ZfasTehran University of Medical SciencesJournal of Dental Medicine1024-641X2008-24442006-06-011918490Evaluation of the effect of three disinfectants on removing HBV contaminationArami STavassoty Kheiri MBashar RNadali MABackground and Aim: Infection control is an important issue in dentistry. Without an efficient infection control, pathogens left on instruments and working surfaces will have potential danger to patients’ health. In this research, antiviral effect of three disinfectants: 0.5% sodium hypochlorite 0.05% sodium hypochlorite and Deconex 50 AF, on HBV was investigated. Materials and Methods: In this interventional (before-after) study; serums of 26 HBV positive patients were analyzed by PCR HBV analysis and 9 contaminated species were obtained to test three disinfectants. 36 agar plates were prepared with the contaminated serums. 27 of the plates were disinfected in 3 separate groups with the above mentioned solutions. Nine remaining plates were not disinfected (control). Swabs wetted by BSAS (Bovine Serum Albumin Sodium Chloride) medium were applied on the surface of the plates and the were kept in the transferred medium and sent to virology-lab of Pasteur Institute. HBV DNA were detected by commercial kit of HBV PCR (polymerase chain reaction) method. Data were analyzed by Cochrane test with p<0.05 as the limit of significance. Results: None of samples disinfected with 0.5% sodium hypochlorite showed contamination. 11/1% of samples disinfected with 0.05% sodium hypochlorite and 44/4% of samples disinfected with Deconex 50 AF remained contaminated. Statistical analysis showed a significant difference between 0.5% sodium hypochlorite and the other groups. Conclusion: Our findings revealed that 0.5% sodium hypochlorite solution is a strong and efficient disinfectant against HBV. Key Words: HBV; Sodium hypochloritehttp://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/2518.pdf&manuscript_id=2518HBVDeconex 50 AFAntiviral effect |
| spellingShingle | Arami S Tavassoty Kheiri M Bashar R Nadali MA Evaluation of the effect of three disinfectants on removing HBV contamination Journal of Dental Medicine HBV Deconex 50 AF Antiviral effect |
| title | Evaluation of the effect of three disinfectants on removing HBV contamination |
| title_full | Evaluation of the effect of three disinfectants on removing HBV contamination |
| title_fullStr | Evaluation of the effect of three disinfectants on removing HBV contamination |
| title_full_unstemmed | Evaluation of the effect of three disinfectants on removing HBV contamination |
| title_short | Evaluation of the effect of three disinfectants on removing HBV contamination |
| title_sort | evaluation of the effect of three disinfectants on removing hbv contamination |
| topic | HBV Deconex 50 AF Antiviral effect |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/2518.pdf&manuscript_id=2518 |
| work_keys_str_mv | AT aramis evaluationoftheeffectofthreedisinfectantsonremovinghbvcontamination AT tavassotykheirim evaluationoftheeffectofthreedisinfectantsonremovinghbvcontamination AT basharr evaluationoftheeffectofthreedisinfectantsonremovinghbvcontamination AT nadalima evaluationoftheeffectofthreedisinfectantsonremovinghbvcontamination |