A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country
Abstract Background Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate fo...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-022-05255-1 |
| _version_ | 1811338870853206016 |
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| author | Juliane Hartke Friederike Reuss Isabelle Marie Kramer Axel Magdeburg Isra Deblauwe Reshma Tuladhar Ishan Gautam Meghnath Dhimal Ruth Müller |
| author_facet | Juliane Hartke Friederike Reuss Isabelle Marie Kramer Axel Magdeburg Isra Deblauwe Reshma Tuladhar Ishan Gautam Meghnath Dhimal Ruth Müller |
| author_sort | Juliane Hartke |
| collection | DOAJ |
| description | Abstract Background Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate for the observed expansion of potential vector species. Cost-effective methods that can be easily implemented in resource-limited settings, e.g. under field conditions, are thus urgently needed to function as an early warning system for vector-borne disease epidemics. Our aim was to enhance entomological capacity in Nepal, a country with endemicity of numerous vector-borne diseases and with frequent outbreaks of dengue fever. Methods We used a field barcoding pipeline based on DNA nanopore sequencing (Oxford Nanopore Technologies) and verified its use for different mosquito life stages and storage methods. We furthermore hosted an online workshop to facilitate knowledge transfer to Nepalese scientific experts from different disciplines. Results The use of the barcoding pipeline could be verified for adult mosquitos and eggs, as well as for homogenized samples, dried specimens, samples that were stored in ethanol and frozen tissue. The transfer of knowledge was successful, as reflected by feedback from the participants and their wish to implement the method. Conclusions Cost effective strategies are urgently needed to assess the likelihood of disease outbreaks. We were able to show that field sequencing provides a solution that is cost-effective, undemanding in its implementation and easy to learn. The knowledge transfer to Nepalese scientific experts from different disciplines provides an opportunity for sustainable implementation of low-cost portable sequencing solutions in Nepal. Graphical Abstract |
| first_indexed | 2024-04-13T18:17:01Z |
| format | Article |
| id | doaj.art-64b5aab0776b42d7a7eb6a5217dfe45c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T18:17:01Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-64b5aab0776b42d7a7eb6a5217dfe45c2022-12-22T02:35:38ZengBMCParasites & Vectors1756-33052022-04-0115111210.1186/s13071-022-05255-1A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic countryJuliane Hartke0Friederike Reuss1Isabelle Marie Kramer2Axel Magdeburg3Isra Deblauwe4Reshma Tuladhar5Ishan Gautam6Meghnath Dhimal7Ruth Müller8Unit Entomology, Institute of Tropical MedicineSenckenberg Biodiversity and Climate Research CentreInstitute of Occupational, Social and Environmental Medicine, Goethe UniversitySenckenberg Biodiversity and Climate Research CentreUnit Entomology, Institute of Tropical MedicineCentral Department of Microbiology, Tribhuvan UniversityNatural History Museum, Tribhuvan UniversityNepal Health Research CouncilUnit Entomology, Institute of Tropical MedicineAbstract Background Vector-borne diseases are on the rise on a global scale, which is anticipated to further accelerate because of anthropogenic climate change. Resource-limited regions are especially hard hit by this increment with the currently implemented surveillance programs being inadequate for the observed expansion of potential vector species. Cost-effective methods that can be easily implemented in resource-limited settings, e.g. under field conditions, are thus urgently needed to function as an early warning system for vector-borne disease epidemics. Our aim was to enhance entomological capacity in Nepal, a country with endemicity of numerous vector-borne diseases and with frequent outbreaks of dengue fever. Methods We used a field barcoding pipeline based on DNA nanopore sequencing (Oxford Nanopore Technologies) and verified its use for different mosquito life stages and storage methods. We furthermore hosted an online workshop to facilitate knowledge transfer to Nepalese scientific experts from different disciplines. Results The use of the barcoding pipeline could be verified for adult mosquitos and eggs, as well as for homogenized samples, dried specimens, samples that were stored in ethanol and frozen tissue. The transfer of knowledge was successful, as reflected by feedback from the participants and their wish to implement the method. Conclusions Cost effective strategies are urgently needed to assess the likelihood of disease outbreaks. We were able to show that field sequencing provides a solution that is cost-effective, undemanding in its implementation and easy to learn. The knowledge transfer to Nepalese scientific experts from different disciplines provides an opportunity for sustainable implementation of low-cost portable sequencing solutions in Nepal. Graphical Abstracthttps://doi.org/10.1186/s13071-022-05255-1Species identificationVBDSurveillanceWebinarLow-costAedes |
| spellingShingle | Juliane Hartke Friederike Reuss Isabelle Marie Kramer Axel Magdeburg Isra Deblauwe Reshma Tuladhar Ishan Gautam Meghnath Dhimal Ruth Müller A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country Parasites & Vectors Species identification VBD Surveillance Webinar Low-cost Aedes |
| title | A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country |
| title_full | A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country |
| title_fullStr | A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country |
| title_full_unstemmed | A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country |
| title_short | A barcoding pipeline for mosquito surveillance in Nepal, a biodiverse dengue-endemic country |
| title_sort | barcoding pipeline for mosquito surveillance in nepal a biodiverse dengue endemic country |
| topic | Species identification VBD Surveillance Webinar Low-cost Aedes |
| url | https://doi.org/10.1186/s13071-022-05255-1 |
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