Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper
Detection of mycotoxins by conventional methods such as ELISA or LC-MS can be expensive and time-consuming. Therefore, paper-based biosensors can be effectively used for on-site analysis, due to their low cost and easy detection procedures. Nevertheless, even when the application of colorimetric met...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-08-01
|
| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180419302028 |
| _version_ | 1818148307329875968 |
|---|---|
| author | V.A. Mirón-Mérida M. Wu Y.Y. Gong Y. Guo M. Holmes R. Ettelaie F.M. Goycoolea |
| author_facet | V.A. Mirón-Mérida M. Wu Y.Y. Gong Y. Guo M. Holmes R. Ettelaie F.M. Goycoolea |
| author_sort | V.A. Mirón-Mérida |
| collection | DOAJ |
| description | Detection of mycotoxins by conventional methods such as ELISA or LC-MS can be expensive and time-consuming. Therefore, paper-based biosensors can be effectively used for on-site analysis, due to their low cost and easy detection procedures. Nevertheless, even when the application of colorimetric methods on paper enhance the simplicity and affordability of multiple determinations, the signal intensity and final readout can be affected by a limited color uniformity. In this work, Ellman’s method for the quantification of aflatoxin B1 was utilized as a model colorimetric assay on paper, in which the test zones were modified with chitosan-immobilized enzyme (AChE). A comparison of the cross-linking effect of genipin on two chitosans of varying molar mass and degree of acetylation, exhibited a greater signal enhancement from the sample with a higher degree of acetylation and molecular weight. |
| first_indexed | 2024-12-11T12:49:04Z |
| format | Article |
| id | doaj.art-64ba365e9e3c478a9f4eccf27e091169 |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-12-11T12:49:04Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-64ba365e9e3c478a9f4eccf27e0911692022-12-22T01:06:44ZengElsevierSensing and Bio-Sensing Research2214-18042020-08-0129100339Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paperV.A. Mirón-Mérida0M. Wu1Y.Y. Gong2Y. Guo3M. Holmes4R. Ettelaie5F.M. Goycoolea6Corresponding author.; School of Food Science and Nutrition, University of Leeds, United KingdomSchool of Food Science and Nutrition, University of Leeds, United KingdomSchool of Food Science and Nutrition, University of Leeds, United KingdomSchool of Food Science and Nutrition, University of Leeds, United KingdomSchool of Food Science and Nutrition, University of Leeds, United KingdomSchool of Food Science and Nutrition, University of Leeds, United KingdomCorresponding author.; School of Food Science and Nutrition, University of Leeds, United KingdomDetection of mycotoxins by conventional methods such as ELISA or LC-MS can be expensive and time-consuming. Therefore, paper-based biosensors can be effectively used for on-site analysis, due to their low cost and easy detection procedures. Nevertheless, even when the application of colorimetric methods on paper enhance the simplicity and affordability of multiple determinations, the signal intensity and final readout can be affected by a limited color uniformity. In this work, Ellman’s method for the quantification of aflatoxin B1 was utilized as a model colorimetric assay on paper, in which the test zones were modified with chitosan-immobilized enzyme (AChE). A comparison of the cross-linking effect of genipin on two chitosans of varying molar mass and degree of acetylation, exhibited a greater signal enhancement from the sample with a higher degree of acetylation and molecular weight.http://www.sciencedirect.com/science/article/pii/S2214180419302028paperchitosancross-linkingaflatoxin B1colorimetric detection |
| spellingShingle | V.A. Mirón-Mérida M. Wu Y.Y. Gong Y. Guo M. Holmes R. Ettelaie F.M. Goycoolea Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper Sensing and Bio-Sensing Research paper chitosan cross-linking aflatoxin B1 colorimetric detection |
| title | Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper |
| title_full | Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper |
| title_fullStr | Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper |
| title_full_unstemmed | Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper |
| title_short | Genipin cross-linked chitosan for signal enhancement in the colorimetric detection of aflatoxin B1 on 3MM chromatography paper |
| title_sort | genipin cross linked chitosan for signal enhancement in the colorimetric detection of aflatoxin b1 on 3mm chromatography paper |
| topic | paper chitosan cross-linking aflatoxin B1 colorimetric detection |
| url | http://www.sciencedirect.com/science/article/pii/S2214180419302028 |
| work_keys_str_mv | AT vamironmerida genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT mwu genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT yygong genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT yguo genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT mholmes genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT rettelaie genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper AT fmgoycoolea genipincrosslinkedchitosanforsignalenhancementinthecolorimetricdetectionofaflatoxinb1on3mmchromatographypaper |