Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms
Subclinical mastitis (SCM) is one of the major economic diseases of dairy cows. In the present cross-sectionalstudy, dairy cows (n=205) of small holding dairy farms were screened using California mastitis test (CMT) andprevalence of 12.19% was recorded. Holstein Friesian and Jersey crossbred cows sh...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2022-02-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/120908 |
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| author | NAGAPPA KARABASANAVAR MANJUNATHA L NAVEENKUMAR G S JEEVAN M N G K SIVARAMAN |
| author_facet | NAGAPPA KARABASANAVAR MANJUNATHA L NAVEENKUMAR G S JEEVAN M N G K SIVARAMAN |
| author_sort | NAGAPPA KARABASANAVAR |
| collection | DOAJ |
| description | Subclinical mastitis (SCM) is one of the major economic diseases of dairy cows. In the present cross-sectionalstudy, dairy cows (n=205) of small holding dairy farms were screened using California mastitis test (CMT) andprevalence of 12.19% was recorded. Holstein Friesian and Jersey crossbred cows showed highest CMT score of 2followed by 3, 1 and T; linear scores of somatic cell counts (SCC) of 3.14±0.08, 4.72±0.25, 6.17±0.12, 7.99±0.11and 9.84±0.45 were observed for CMT scores N, T, 1, 2 and 3, respectively. Risk factors associated with SCM werestudied in 25 randomly selected cows. Staphylococcus species were detected in 54% of quarters based on PCRamplification of 16s rRNA gene; thermonuclease (nuc gene) PCR categorized Staphylococcus species as S. aureus(SA, 37%) and non-aureus Staphylococci (NAS, 26%). Significant association between CMT score 2 andStaphylococcus species was established. Staphylococcus species, SA and NAS, were detected in 30.7, 20.5 and17.9% of SCM negative as well as 68.85, 47.54 and 31.14% SCM positive quarters, respectively. Significantassociation between SCM and Staphylococcus species and Staphylococcus aureus was observed. Study indicatedstrong association between SCM, Staphylococcus aureus and certain risk factors among cows reared under smallholding dairy farms of the tropical region. |
| first_indexed | 2024-04-10T16:35:38Z |
| format | Article |
| id | doaj.art-64bb78c6701749edbfd9354fdc93a1ca |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:35:38Z |
| publishDate | 2022-02-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-64bb78c6701749edbfd9354fdc93a1ca2023-02-08T11:30:25ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-02-0192110.56093/ijans.v92i1.120908Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farmsNAGAPPA KARABASANAVAR0MANJUNATHA L1NAVEENKUMAR G S2JEEVAN M N3G K SIVARAMAN4Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 IndiaKarnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 IndiaKarnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 IndiaKarnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 IndiaKarnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 IndiaSubclinical mastitis (SCM) is one of the major economic diseases of dairy cows. In the present cross-sectionalstudy, dairy cows (n=205) of small holding dairy farms were screened using California mastitis test (CMT) andprevalence of 12.19% was recorded. Holstein Friesian and Jersey crossbred cows showed highest CMT score of 2followed by 3, 1 and T; linear scores of somatic cell counts (SCC) of 3.14±0.08, 4.72±0.25, 6.17±0.12, 7.99±0.11and 9.84±0.45 were observed for CMT scores N, T, 1, 2 and 3, respectively. Risk factors associated with SCM werestudied in 25 randomly selected cows. Staphylococcus species were detected in 54% of quarters based on PCRamplification of 16s rRNA gene; thermonuclease (nuc gene) PCR categorized Staphylococcus species as S. aureus(SA, 37%) and non-aureus Staphylococci (NAS, 26%). Significant association between CMT score 2 andStaphylococcus species was established. Staphylococcus species, SA and NAS, were detected in 30.7, 20.5 and17.9% of SCM negative as well as 68.85, 47.54 and 31.14% SCM positive quarters, respectively. Significantassociation between SCM and Staphylococcus species and Staphylococcus aureus was observed. Study indicatedstrong association between SCM, Staphylococcus aureus and certain risk factors among cows reared under smallholding dairy farms of the tropical region.https://epubs.icar.org.in/index.php/IJAnS/article/view/120908AssociationCrossbred cowPCRStaphylococcusSubclinical mastitis |
| spellingShingle | NAGAPPA KARABASANAVAR MANJUNATHA L NAVEENKUMAR G S JEEVAN M N G K SIVARAMAN Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms Indian Journal of Animal Sciences Association Crossbred cow PCR Staphylococcus Subclinical mastitis |
| title | Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms |
| title_full | Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms |
| title_fullStr | Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms |
| title_full_unstemmed | Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms |
| title_short | Subclinical mastitis in crossbred dairy cows: Staphylococcus spp. and risk factors in small holder dairy farms |
| title_sort | subclinical mastitis in crossbred dairy cows staphylococcus spp and risk factors in small holder dairy farms |
| topic | Association Crossbred cow PCR Staphylococcus Subclinical mastitis |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/120908 |
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