Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress
As the dominant natural enemy of aphids, <i>Harmonia axyridis</i> plays a crucial role in integrated pest control (IPM) in agro-ecosystems. In order to study the physiological adaptation and regulation ability of <i>Harmonia axyridis</i> to insecticides under chemical pestici...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/12/8/1134 |
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| author | Guoyong Li Xiaoli Ni Meikun Liu Bing Tang Can Li Yangyang Liu |
| author_facet | Guoyong Li Xiaoli Ni Meikun Liu Bing Tang Can Li Yangyang Liu |
| author_sort | Guoyong Li |
| collection | DOAJ |
| description | As the dominant natural enemy of aphids, <i>Harmonia axyridis</i> plays a crucial role in integrated pest control (IPM) in agro-ecosystems. In order to study the physiological adaptation and regulation ability of <i>Harmonia axyridis</i> to insecticides under chemical pesticide stress, ladybirds were treated with organophosphorus chlorpyrifos (chlorpyrifos) and new nicotine imidacloprid (imidacloprid) to explore the physiological adaptability of ladybirds under chemical pesticide stress by activating trehalose metabolism. The results showed that the imidacloprid affect the larvae develop to pupate, resulted in the <i>H. axyridis</i> died and significantly increased the food consumption of <i>Harmonia axyridis</i>, while the chlorpyrifos prolong the development period of pupae significantly and decreased significantly the food intake of <i>H. axyridis</i> fed with aphids treated with chlorpyrifos. It was further found that Chlorpyrifos could inhibit the activity of the trehalase, while the trehalase activity increased under imidacloprid stress, but both insecticides could decrease the trehalose content. The TRE and TPS genes of <i>Harmonia axyridis</i> under chlorpyrifos and imidacloprid stress were upregulated or downregulated. These relevant results can provide a strong reference for the rational use of chemical pesticides or biological pesticides to control pests in the future. |
| first_indexed | 2024-03-09T12:03:41Z |
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| id | doaj.art-64be87dee6c54e19b3a6d31af5f97b6a |
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| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T12:03:41Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-64be87dee6c54e19b3a6d31af5f97b6a2023-11-30T23:00:42ZengMDPI AGAgriculture2077-04722022-07-01128113410.3390/agriculture12081134Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid StressGuoyong Li0Xiaoli Ni1Meikun Liu2Bing Tang3Can Li4Yangyang Liu5Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of Mountainous Region, Guiyang University, Guiyang 550005, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insects of Mountainous Region, Guiyang University, Guiyang 550005, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insects of Mountainous Region, Guiyang University, Guiyang 550005, ChinaGuizhou Provincial Key Laboratory for Rare Animal and Economic Insects of Mountainous Region, Guiyang University, Guiyang 550005, ChinaAs the dominant natural enemy of aphids, <i>Harmonia axyridis</i> plays a crucial role in integrated pest control (IPM) in agro-ecosystems. In order to study the physiological adaptation and regulation ability of <i>Harmonia axyridis</i> to insecticides under chemical pesticide stress, ladybirds were treated with organophosphorus chlorpyrifos (chlorpyrifos) and new nicotine imidacloprid (imidacloprid) to explore the physiological adaptability of ladybirds under chemical pesticide stress by activating trehalose metabolism. The results showed that the imidacloprid affect the larvae develop to pupate, resulted in the <i>H. axyridis</i> died and significantly increased the food consumption of <i>Harmonia axyridis</i>, while the chlorpyrifos prolong the development period of pupae significantly and decreased significantly the food intake of <i>H. axyridis</i> fed with aphids treated with chlorpyrifos. It was further found that Chlorpyrifos could inhibit the activity of the trehalase, while the trehalase activity increased under imidacloprid stress, but both insecticides could decrease the trehalose content. The TRE and TPS genes of <i>Harmonia axyridis</i> under chlorpyrifos and imidacloprid stress were upregulated or downregulated. These relevant results can provide a strong reference for the rational use of chemical pesticides or biological pesticides to control pests in the future.https://www.mdpi.com/2077-0472/12/8/1134<i>Harmonia axyridis</i>chlorpyrifosimidaclopridphenotypetrehalose and energy metabolismqPCR |
| spellingShingle | Guoyong Li Xiaoli Ni Meikun Liu Bing Tang Can Li Yangyang Liu Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress Agriculture <i>Harmonia axyridis</i> chlorpyrifos imidacloprid phenotype trehalose and energy metabolism qPCR |
| title | Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress |
| title_full | Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress |
| title_fullStr | Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress |
| title_full_unstemmed | Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress |
| title_short | Changes in the Physiological Adaptation and Regulation Ability in <i>Harmonia axyridis</i> under Chlorpyrifos and Imidacloprid Stress |
| title_sort | changes in the physiological adaptation and regulation ability in i harmonia axyridis i under chlorpyrifos and imidacloprid stress |
| topic | <i>Harmonia axyridis</i> chlorpyrifos imidacloprid phenotype trehalose and energy metabolism qPCR |
| url | https://www.mdpi.com/2077-0472/12/8/1134 |
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