<i>FtbZIP85</i> Is Involved in the Accumulation of Proanthocyanidin by Regulating the Transcription of <i>FtDFR</i> in Tartary Buckwheat
As a drought-tolerant crop, Tartary buckwheat survives under adverse environmental conditions, including drought stress. Proanthocyanidins (PAs) and anthocyanins are flavonoid compounds, and they participate in the regulation of resistance to both biotic and abiotic stresses by triggering genes’ bio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/4/221 |
| Summary: | As a drought-tolerant crop, Tartary buckwheat survives under adverse environmental conditions, including drought stress. Proanthocyanidins (PAs) and anthocyanins are flavonoid compounds, and they participate in the regulation of resistance to both biotic and abiotic stresses by triggering genes’ biosynthesis of flavonoids. In this study, a basic leucine zipper, basic leucine zipper 85 (<i>FtbZIP85</i>), which was predominantly expressed in seeds, was isolated from Tartary buckwheat. Our study shows that the expressions of <i>FtDFR</i>, <i>FtbZIP85</i> and <i>FtSnRK2.6</i> were tissue-specific and located in both the nucleus and the cytosol. FtbZIP85 could positively regulate PA biosynthesis by binding to the ABA-responsive element (ABRE) in the promoter of dihydroflavonol 4-reductase (<i>FtDFR</i>), which is a key enzyme in the phenylpropanoid biosynthetic pathway. Additionally, FtbZIP85 was also involved in the regulation of PA biosynthesis via interactions with FtSnRK2.6 but not with FtSnRK2.2/2.3. This study reveals that <i>FtbZIP85</i> is a positive regulator of PA biosynthesis in TB. |
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| ISSN: | 1467-3037 1467-3045 |