Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models
Bone is a site of distant metastases, which are a common cause of morbidity and mortality with a high socio-economic impact, for many malignant tumours. In order to engineer pharmacological therapies that are suitable for this debilitating disease, this experimental work presents injectable lipid na...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/14/2/238 |
| _version_ | 1827344001429143552 |
|---|---|
| author | Ian Stoppa Chiara Dianzani Nausicaa Clemente Annalisa Bozza Valentina Bordano Sara Garelli Luigi Cangemi Umberto Dianzani Luigi Battaglia |
| author_facet | Ian Stoppa Chiara Dianzani Nausicaa Clemente Annalisa Bozza Valentina Bordano Sara Garelli Luigi Cangemi Umberto Dianzani Luigi Battaglia |
| author_sort | Ian Stoppa |
| collection | DOAJ |
| description | Bone is a site of distant metastases, which are a common cause of morbidity and mortality with a high socio-economic impact, for many malignant tumours. In order to engineer pharmacological therapies that are suitable for this debilitating disease, this experimental work presents injectable lipid nanoemulsions, which are endowed with a long history of safe clinical usage in parenteral nutrition, their loading with vincristine and their grafting with alendronate, with a dual purpose: merging the anticancer activity of bisphosphonates and vincristine, and enhancing bone-targeted delivery. In cell studies, alendronate synergised with the anti-migration activity of vincristine, which is important as migration plays a key role in the metastatisation process. In preliminary animal studies, carried out thanks to IVIS technology, alendronate conjugation enhanced the bone targeting of fluorescently labelled nanoemulsions. These encouraging results will drive further studies on suitable animal models of the disease. |
| first_indexed | 2024-03-07T22:40:40Z |
| format | Article |
| id | doaj.art-64c0d3947aa24a85969c2d506d96458c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-07T22:40:40Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-64c0d3947aa24a85969c2d506d96458c2024-02-23T15:09:27ZengMDPI AGBiomolecules2218-273X2024-02-0114223810.3390/biom14020238Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal ModelsIan Stoppa0Chiara Dianzani1Nausicaa Clemente2Annalisa Bozza3Valentina Bordano4Sara Garelli5Luigi Cangemi6Umberto Dianzani7Luigi Battaglia8Department of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases (IRCAD), University of Eastern Piedmont (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyDepartment of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases (IRCAD), University of Eastern Piedmont (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyDepartment of Health Sciences, Interdisciplinary Research Center of Autoimmune Diseases (IRCAD), University of Eastern Piedmont (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Drug Science and Technology, University of Turin, via Pietro Giuria 9, 10125 Turin, ItalyBone is a site of distant metastases, which are a common cause of morbidity and mortality with a high socio-economic impact, for many malignant tumours. In order to engineer pharmacological therapies that are suitable for this debilitating disease, this experimental work presents injectable lipid nanoemulsions, which are endowed with a long history of safe clinical usage in parenteral nutrition, their loading with vincristine and their grafting with alendronate, with a dual purpose: merging the anticancer activity of bisphosphonates and vincristine, and enhancing bone-targeted delivery. In cell studies, alendronate synergised with the anti-migration activity of vincristine, which is important as migration plays a key role in the metastatisation process. In preliminary animal studies, carried out thanks to IVIS technology, alendronate conjugation enhanced the bone targeting of fluorescently labelled nanoemulsions. These encouraging results will drive further studies on suitable animal models of the disease.https://www.mdpi.com/2218-273X/14/2/238nanoemulsionalendronatevincristine |
| spellingShingle | Ian Stoppa Chiara Dianzani Nausicaa Clemente Annalisa Bozza Valentina Bordano Sara Garelli Luigi Cangemi Umberto Dianzani Luigi Battaglia Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models Biomolecules nanoemulsion alendronate vincristine |
| title | Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models |
| title_full | Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models |
| title_fullStr | Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models |
| title_full_unstemmed | Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models |
| title_short | Alendronate-Grafted Nanoemulsions for Bone-Targeted Vincristine Delivery: Preliminary Studies on Cell and Animal Models |
| title_sort | alendronate grafted nanoemulsions for bone targeted vincristine delivery preliminary studies on cell and animal models |
| topic | nanoemulsion alendronate vincristine |
| url | https://www.mdpi.com/2218-273X/14/2/238 |
| work_keys_str_mv | AT ianstoppa alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT chiaradianzani alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT nausicaaclemente alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT annalisabozza alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT valentinabordano alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT saragarelli alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT luigicangemi alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT umbertodianzani alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels AT luigibattaglia alendronategraftednanoemulsionsforbonetargetedvincristinedeliverypreliminarystudiesoncellandanimalmodels |