Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates
A combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline <i>H. heilmannii</i> and <i>H. ailurogastricus</i> isolates. All <i>H. ailurogastricus</i> isolates showed a monomodal distribution of MICs...
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/957 |
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| author | Rita Matos Chloë De Witte Annemieke Smet Helena Berlamont Sofie De Bruyckere Irina Amorim Fátima Gärtner Freddy Haesebrouck |
| author_facet | Rita Matos Chloë De Witte Annemieke Smet Helena Berlamont Sofie De Bruyckere Irina Amorim Fátima Gärtner Freddy Haesebrouck |
| author_sort | Rita Matos |
| collection | DOAJ |
| description | A combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline <i>H. heilmannii</i> and <i>H. ailurogastricus</i> isolates. All <i>H. ailurogastricus</i> isolates showed a monomodal distribution of MICs for all the antimicrobial agents tested. For <i>H. heilmannii</i>, a bimodal distribution was observed for azithromycin, enrofloxacin, spectinomycin, and lincomycin. Single nucleotide polymorphisms (SNPs) were found in 50S ribosomal proteins L2 and L3 of the <i>H. heilmannii</i> isolate not belonging to the WT population for azithromycin, and in 30S ribosomal proteins S1, S7, and S12 of the isolate not belonging to the WT population for spectinomycin. The antimicrobial resistance mechanism to enrofloxacin and lincomycin remains unknown (2 and 1 <i>H. heilmannii</i> isolate(s), resp.). Furthermore, <i>H. heilmannii</i> isolates showed higher MICs for neomycin compared to <i>H. ailurogastricus</i> isolates which may be related to the presence of SNPs in several 30S and 50S ribosomal protein encoding genes and ribosomal RNA methyltransferase genes. This study shows that acquired resistance to azithromycin, spectinomycin, enrofloxacin, and lincomycin occasionally occurs in feline <i>H. heilmannii</i> isolates. As pets may constitute a source of infection for humans, this should be kept in mind when dealing with a human patient infected with <i>H. heilmannii</i>. |
| first_indexed | 2024-03-10T18:53:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T18:53:16Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-64c197bad1dd4e0585317ed108ff73eb2023-11-20T04:58:59ZengMDPI AGMicroorganisms2076-26072020-06-018695710.3390/microorganisms8060957Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> IsolatesRita Matos0Chloë De Witte1Annemieke Smet2Helena Berlamont3Sofie De Bruyckere4Irina Amorim5Fátima Gärtner6Freddy Haesebrouck7Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, PortugalDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, BelgiumTranslational Research in Immunology and Inflammation, Laboratory of Experimental Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Antwerp University, B-2610 Antwerp, BelgiumDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, BelgiumInstituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, PortugalInstituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, PortugalDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, BelgiumA combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline <i>H. heilmannii</i> and <i>H. ailurogastricus</i> isolates. All <i>H. ailurogastricus</i> isolates showed a monomodal distribution of MICs for all the antimicrobial agents tested. For <i>H. heilmannii</i>, a bimodal distribution was observed for azithromycin, enrofloxacin, spectinomycin, and lincomycin. Single nucleotide polymorphisms (SNPs) were found in 50S ribosomal proteins L2 and L3 of the <i>H. heilmannii</i> isolate not belonging to the WT population for azithromycin, and in 30S ribosomal proteins S1, S7, and S12 of the isolate not belonging to the WT population for spectinomycin. The antimicrobial resistance mechanism to enrofloxacin and lincomycin remains unknown (2 and 1 <i>H. heilmannii</i> isolate(s), resp.). Furthermore, <i>H. heilmannii</i> isolates showed higher MICs for neomycin compared to <i>H. ailurogastricus</i> isolates which may be related to the presence of SNPs in several 30S and 50S ribosomal protein encoding genes and ribosomal RNA methyltransferase genes. This study shows that acquired resistance to azithromycin, spectinomycin, enrofloxacin, and lincomycin occasionally occurs in feline <i>H. heilmannii</i> isolates. As pets may constitute a source of infection for humans, this should be kept in mind when dealing with a human patient infected with <i>H. heilmannii</i>.https://www.mdpi.com/2076-2607/8/6/957<i>Helicobacter heilmannii</i><i>Helicobacter ailurogastricus</i>antimicrobial susceptibilityzoonosesgastric diseaseresistance mechanisms |
| spellingShingle | Rita Matos Chloë De Witte Annemieke Smet Helena Berlamont Sofie De Bruyckere Irina Amorim Fátima Gärtner Freddy Haesebrouck Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates Microorganisms <i>Helicobacter heilmannii</i> <i>Helicobacter ailurogastricus</i> antimicrobial susceptibility zoonoses gastric disease resistance mechanisms |
| title | Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates |
| title_full | Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates |
| title_fullStr | Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates |
| title_full_unstemmed | Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates |
| title_short | Antimicrobial Susceptibility Pattern of <i>Helicobacter heilmannii</i> and <i>Helicobacter ailurogastricus</i> Isolates |
| title_sort | antimicrobial susceptibility pattern of i helicobacter heilmannii i and i helicobacter ailurogastricus i isolates |
| topic | <i>Helicobacter heilmannii</i> <i>Helicobacter ailurogastricus</i> antimicrobial susceptibility zoonoses gastric disease resistance mechanisms |
| url | https://www.mdpi.com/2076-2607/8/6/957 |
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