Concentrated ambient fine particles exposure affects ovarian follicle development in mice
Background: Ambient fine particles (PM2.5) are known to cause various reproductive and developmental diseases. However, the potential mechanisms of PM2.5 exposure induced female reproductive damage remain unclear. Methods: Four weeks old female C57BL/6 J mice were exposed to filtered air (FA, n = 10...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322000185 |
| _version_ | 1798018320237068288 |
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| author | Mingjun Yang Fang Tian Shimin Tao Minjie Xia Yuzhu Wang Jingying Hu Bin Pan Zhouzhou Li Renzhen Peng Haidong Kan Yanyi Xu Weihua Li |
| author_facet | Mingjun Yang Fang Tian Shimin Tao Minjie Xia Yuzhu Wang Jingying Hu Bin Pan Zhouzhou Li Renzhen Peng Haidong Kan Yanyi Xu Weihua Li |
| author_sort | Mingjun Yang |
| collection | DOAJ |
| description | Background: Ambient fine particles (PM2.5) are known to cause various reproductive and developmental diseases. However, the potential mechanisms of PM2.5 exposure induced female reproductive damage remain unclear. Methods: Four weeks old female C57BL/6 J mice were exposed to filtered air (FA, n = 10) or concentrated ambient PM2.5 (CAP, n = 10) using a versatile aerosol concentration enrichment system. After 9 weeks of the exposure, mice were sacrificed under sevoflurane anesthesia and tissue samples were collected. Immunohistochemical analysis, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, and RNA-sequencing were performed to analyze the effects of PM2.5 exposure on follicle development and elucidate its potential mechanisms. Results: Chronic PM2.5 exposure resulted in follicular dysplasia. Compared to the FA-exposed group, follicular atresia in the CAP-exposed mice were significantly increased. Further studies confirmed that CAP induced apoptosis in granulosa cells, accompanied by a distortion of hormone homeostasis. In addition, RNA-sequencing data demonstrated that CAP exposure induced the alteration of ovarian gene expressions and was associated with inflammatory response. Conclusions: Chronic exposure to CAP can induce follicular atresia, which was associated with hormone modulation and inflammation. |
| first_indexed | 2024-04-11T16:22:11Z |
| format | Article |
| id | doaj.art-64c683e3fbd44f58bbb87f098c9ddd4e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T16:22:11Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-64c683e3fbd44f58bbb87f098c9ddd4e2022-12-22T04:14:19ZengElsevierEcotoxicology and Environmental Safety0147-65132022-02-01231113178Concentrated ambient fine particles exposure affects ovarian follicle development in miceMingjun Yang0Fang Tian1Shimin Tao2Minjie Xia3Yuzhu Wang4Jingying Hu5Bin Pan6Zhouzhou Li7Renzhen Peng8Haidong Kan9Yanyi Xu10Weihua Li11Key Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaKey Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaKey Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaKey Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaKey Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaKey Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, ChinaDepartment of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China; Corresponding authors.Key Laboratory of Reproduction Regulation of National Health Commission (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, China; Corresponding authors.Background: Ambient fine particles (PM2.5) are known to cause various reproductive and developmental diseases. However, the potential mechanisms of PM2.5 exposure induced female reproductive damage remain unclear. Methods: Four weeks old female C57BL/6 J mice were exposed to filtered air (FA, n = 10) or concentrated ambient PM2.5 (CAP, n = 10) using a versatile aerosol concentration enrichment system. After 9 weeks of the exposure, mice were sacrificed under sevoflurane anesthesia and tissue samples were collected. Immunohistochemical analysis, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, and RNA-sequencing were performed to analyze the effects of PM2.5 exposure on follicle development and elucidate its potential mechanisms. Results: Chronic PM2.5 exposure resulted in follicular dysplasia. Compared to the FA-exposed group, follicular atresia in the CAP-exposed mice were significantly increased. Further studies confirmed that CAP induced apoptosis in granulosa cells, accompanied by a distortion of hormone homeostasis. In addition, RNA-sequencing data demonstrated that CAP exposure induced the alteration of ovarian gene expressions and was associated with inflammatory response. Conclusions: Chronic exposure to CAP can induce follicular atresia, which was associated with hormone modulation and inflammation.http://www.sciencedirect.com/science/article/pii/S0147651322000185Ambient PM2.5Ovarian dysfunctionFollicular atresiaApoptosisInflammatory response |
| spellingShingle | Mingjun Yang Fang Tian Shimin Tao Minjie Xia Yuzhu Wang Jingying Hu Bin Pan Zhouzhou Li Renzhen Peng Haidong Kan Yanyi Xu Weihua Li Concentrated ambient fine particles exposure affects ovarian follicle development in mice Ecotoxicology and Environmental Safety Ambient PM2.5 Ovarian dysfunction Follicular atresia Apoptosis Inflammatory response |
| title | Concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| title_full | Concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| title_fullStr | Concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| title_full_unstemmed | Concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| title_short | Concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| title_sort | concentrated ambient fine particles exposure affects ovarian follicle development in mice |
| topic | Ambient PM2.5 Ovarian dysfunction Follicular atresia Apoptosis Inflammatory response |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322000185 |
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