PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram)
ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets...
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| Format: | Article |
| Language: | Indonesian |
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Syiah Kuala University
2016-09-01
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| Series: | Jurnal Kedokteran Hewan |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/5139 |
| _version_ | 1797278985037545472 |
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| author | Herdis Herdis I Wayan Angga Darmawan Muhammad Rizal |
| author_facet | Herdis Herdis I Wayan Angga Darmawan Muhammad Rizal |
| author_sort | Herdis Herdis |
| collection | DOAJ |
| description | ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram. |
| first_indexed | 2024-03-07T16:16:53Z |
| format | Article |
| id | doaj.art-64c728d9b0ad417d848e19b6b71b0b8b |
| institution | Directory Open Access Journal |
| issn | 1978-225X 2502-5600 |
| language | Indonesian |
| last_indexed | 2024-03-07T16:16:53Z |
| publishDate | 2016-09-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | Jurnal Kedokteran Hewan |
| spelling | doaj.art-64c728d9b0ad417d848e19b6b71b0b8b2024-03-04T10:29:00ZindSyiah Kuala UniversityJurnal Kedokteran Hewan1978-225X2502-56002016-09-0110220020410.21157/j.ked.hewan.v10i2.51394307PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram)Herdis Herdis0I Wayan Angga Darmawan1Muhammad Rizal2Pusat Teknologi Produksi Pertanian Badan Pengkajian dan Penerapan Teknologi, LAPTIAB BantenPusat Teknologi Produksi Pertanian Badan Pengkajian dan Penerapan Teknologi, LAPTIAB BantenProgram Studi Peternakan, Fakultas Pertanian, Universitas Lambung Mangkurat, Banjar BaruABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram.https://jurnal.usk.ac.id/JKH/article/view/5139ramepididymal spermotozoasucrose |
| spellingShingle | Herdis Herdis I Wayan Angga Darmawan Muhammad Rizal PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) Jurnal Kedokteran Hewan ram epididymal spermotozoa sucrose |
| title | PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) |
| title_full | PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) |
| title_fullStr | PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) |
| title_full_unstemmed | PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) |
| title_short | PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) |
| title_sort | penambahan beberapa jenis gula dapat meningkatkan kualitas spermatozoa beku asal epididimis ternak domba addition of various sugars in improving quality of frozen thawed epididymal spermatozoa of ram |
| topic | ram epididymal spermotozoa sucrose |
| url | https://jurnal.usk.ac.id/JKH/article/view/5139 |
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