Mutation Hotspot for Changing the Substrate Specificity of β-<i>N</i>-Acetylhexosaminidase: A Library of GlcNAcases

Mutation Hotspot for Changing the Substrate Specificity of β-<i>N</i>-Acetylhexosaminidase: A Library of GlcNAcases

β-<i>N</i>-Acetylhexosaminidase from <i>Talaromyces flavus</i> (<i>Tf</i>Hex; EC 3.2.1.52) is an <i>exo</i>-glycosidase with dual activity for cleaving <i>N</i>-acetylglucosamine (GlcNAc) and <i>N</i>-acetylgalactosamine (GalNAc...

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Bibliographic Details
Main Authors: Pavlína Nekvasilová, Natalia Kulik, Michael Kotik, Lucie Petrásková, Kristýna Slámová, Vladimír Křen, Pavla Bojarová
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
β-<i>N</i>-acetylhexosaminidase
<i>Talaromyces flavus</i>
<i>Pichia pastoris</i>
site-saturation mutagenesis
site-directed mutagenesis
substrate specificity
Online Access:https://www.mdpi.com/1422-0067/23/20/12456

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