The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes
To increase the productivity of in vitro development, the antioxidants have been used for culture system of bovine oocytes and embryos. However, comparative studies on these molecules are rare and direct beneficial effects on blastocyst production cannot be discriminated for best results. The study...
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The Korean Society of Animal Reproduction and Biotechnology
2017-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
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| Online Access: | http://www.e-jarb.org/journal/view.html?uid=19&vmd=Full |
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| author | Min-Su Kim Chan-Lan Kim Namtea Kim Ik Soo Jeon Sung Woo Kim |
| author_facet | Min-Su Kim Chan-Lan Kim Namtea Kim Ik Soo Jeon Sung Woo Kim |
| author_sort | Min-Su Kim |
| collection | DOAJ |
| description | To increase the productivity of in vitro development, the antioxidants have been used for culture system of bovine oocytes and embryos. However, comparative studies on these molecules are rare and direct beneficial effects on blastocyst production cannot be discriminated for best results. The study was conducted to determine the influence of N-acetyl-L-cysteine (NAC), N-acetyl-L-cysteine amide (NACA), glutathione (GSH) and cysteamime (CYS) on maturation competence of COCs from GV to MII stage and productivity of blastocyst formation during in vitro fertilization and culture. There was no difference among maturation rates of oocytes to metaphase II with polar body with antioxidants for any of the treatment groups (p>0.05). However, the significant improvement on the rate of blastocysts (32.3±5.0%) was found in 0.1 mM CYS treatment than 0.3 mM NAC, 0.2 mM NACA or 0.5mM GSH (p<0.05). The addition of NAC (18.8±3.7%) or NACA (21.2±3.9%) did not improve development competence to morula and blastocysts than control (24.4±4.1%) and GSH (26.5±5.0%) (p>0.05). Our study showed that medium supplementation with CYS during IVM and IVC improved the rate of bovine embryo development but not with NAC, NACA and GSH addition. |
| first_indexed | 2024-12-14T21:31:11Z |
| format | Article |
| id | doaj.art-64c9e73bc950458aa836f456789f6db6 |
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| issn | 2671-4639 2671-4663 |
| language | English |
| last_indexed | 2024-12-14T21:31:11Z |
| publishDate | 2017-09-01 |
| publisher | The Korean Society of Animal Reproduction and Biotechnology |
| record_format | Article |
| series | Journal of Animal Reproduction and Biotechnology |
| spelling | doaj.art-64c9e73bc950458aa836f456789f6db62022-12-21T22:46:42ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632017-09-0132320120710.12750/JET.2017.32.3.201The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine OocytesMin-Su Kim0Chan-Lan Kim1Namtea Kim2Ik Soo Jeon3Sung Woo Kim4Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaAnimal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, KoreaTo increase the productivity of in vitro development, the antioxidants have been used for culture system of bovine oocytes and embryos. However, comparative studies on these molecules are rare and direct beneficial effects on blastocyst production cannot be discriminated for best results. The study was conducted to determine the influence of N-acetyl-L-cysteine (NAC), N-acetyl-L-cysteine amide (NACA), glutathione (GSH) and cysteamime (CYS) on maturation competence of COCs from GV to MII stage and productivity of blastocyst formation during in vitro fertilization and culture. There was no difference among maturation rates of oocytes to metaphase II with polar body with antioxidants for any of the treatment groups (p>0.05). However, the significant improvement on the rate of blastocysts (32.3±5.0%) was found in 0.1 mM CYS treatment than 0.3 mM NAC, 0.2 mM NACA or 0.5mM GSH (p<0.05). The addition of NAC (18.8±3.7%) or NACA (21.2±3.9%) did not improve development competence to morula and blastocysts than control (24.4±4.1%) and GSH (26.5±5.0%) (p>0.05). Our study showed that medium supplementation with CYS during IVM and IVC improved the rate of bovine embryo development but not with NAC, NACA and GSH addition.http://www.e-jarb.org/journal/view.html?uid=19&vmd=Fullantioxidantsbovine embryoin vitro development |
| spellingShingle | Min-Su Kim Chan-Lan Kim Namtea Kim Ik Soo Jeon Sung Woo Kim The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes Journal of Animal Reproduction and Biotechnology antioxidants bovine embryo in vitro development |
| title | The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes |
| title_full | The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes |
| title_fullStr | The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes |
| title_full_unstemmed | The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes |
| title_short | The Effects of Antioxidants, N-acetyl-L-cystein, N-acetyl-L-cystein Amide, Glutathione or Cysteamine on the Development of in vitro Fertilized bovine Oocytes |
| title_sort | effects of antioxidants n acetyl l cystein n acetyl l cystein amide glutathione or cysteamine on the development of in vitro fertilized bovine oocytes |
| topic | antioxidants bovine embryo in vitro development |
| url | http://www.e-jarb.org/journal/view.html?uid=19&vmd=Full |
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