Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells
The tumor suppressor gene, p53, is inactivated in the human hepatocellular carcinoma cells line, Hep3B. Berberine has been reported to inhibit the proliferation of cancer cells. This study examined whether apoptosis was induced in berberine-treated Hep3B cells and observed the association between ap...
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The Korean Society for Clinical Laboratory Science
2020-03-01
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| Series: | Korean Journal of Clinical Laboratory Science |
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| _version_ | 1818108302883553280 |
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| author | Dae-Yeon Kim Seon-Hyoung Kim Hee-Tae Cheong Chang-Six Ra Ki-Jong Rhee Bae Dong Jung |
| author_facet | Dae-Yeon Kim Seon-Hyoung Kim Hee-Tae Cheong Chang-Six Ra Ki-Jong Rhee Bae Dong Jung |
| author_sort | Dae-Yeon Kim |
| collection | DOAJ |
| description | The tumor suppressor gene, p53, is inactivated in the human hepatocellular carcinoma cells line, Hep3B. Berberine has been reported to inhibit the proliferation of cancer cells. This study examined whether apoptosis was induced in berberine-treated Hep3B cells and observed the association between apoptosis and the expression of p53 and DNA methyltransferase (DNMT). The cell viability was measured using an MTT assay. Apoptosis of Hep3B was measured using annexin V flow cytometry. Berberine-treated cells were examined for their DNMT enzymatic activity, mRNA expression, and protein synthesis. The p53 levels were examined by Western blot analysis. The berberine treatment resulted in increased Hep3B cell death and apoptosis in a time- and dose-dependent manner. The DNMT3b activity, mRNA expression, and protein levels all decreased after the berberine treatment. In contrast, the p53 protein levels increased with a concomitant decrease in DNMT3b. No change in the expression of ERK was observed, but the P-ERK levels decreased in a dose dependent manner. These results indicate that a treatment of Hep3B cells with berberine can reduce the expression of DNMT3b, leading to an increase in the tumor suppressant gene p53 and an increase in cell apoptosis. This shows that berberine can effectively suppress the proliferation of liver cancer cells. |
| first_indexed | 2024-12-11T02:13:12Z |
| format | Article |
| id | doaj.art-64cb851dc527435f97dc882e607b68c6 |
| institution | Directory Open Access Journal |
| issn | 1738-3544 2288-1662 |
| language | English |
| last_indexed | 2024-12-11T02:13:12Z |
| publishDate | 2020-03-01 |
| publisher | The Korean Society for Clinical Laboratory Science |
| record_format | Article |
| series | Korean Journal of Clinical Laboratory Science |
| spelling | doaj.art-64cb851dc527435f97dc882e607b68c62022-12-22T01:24:14ZengThe Korean Society for Clinical Laboratory ScienceKorean Journal of Clinical Laboratory Science1738-35442288-16622020-03-01521697710.15324/kjcls.2020.52.1.69Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B CellsDae-Yeon Kim0Seon-Hyoung Kim1Hee-Tae Cheong2Chang-Six Ra3Ki-Jong Rhee4Bae Dong Jung5https://orcid.org/0000-0002-6653-9928College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, KoreaCollege of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, KoreaCollege of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, KoreaCollege of Animal Life Sciences, Kangwon National University, Chuncheon, KoreaDepartment of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju, KoreaCollege of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, Chuncheon, KoreaThe tumor suppressor gene, p53, is inactivated in the human hepatocellular carcinoma cells line, Hep3B. Berberine has been reported to inhibit the proliferation of cancer cells. This study examined whether apoptosis was induced in berberine-treated Hep3B cells and observed the association between apoptosis and the expression of p53 and DNA methyltransferase (DNMT). The cell viability was measured using an MTT assay. Apoptosis of Hep3B was measured using annexin V flow cytometry. Berberine-treated cells were examined for their DNMT enzymatic activity, mRNA expression, and protein synthesis. The p53 levels were examined by Western blot analysis. The berberine treatment resulted in increased Hep3B cell death and apoptosis in a time- and dose-dependent manner. The DNMT3b activity, mRNA expression, and protein levels all decreased after the berberine treatment. In contrast, the p53 protein levels increased with a concomitant decrease in DNMT3b. No change in the expression of ERK was observed, but the P-ERK levels decreased in a dose dependent manner. These results indicate that a treatment of Hep3B cells with berberine can reduce the expression of DNMT3b, leading to an increase in the tumor suppressant gene p53 and an increase in cell apoptosis. This shows that berberine can effectively suppress the proliferation of liver cancer cells.apoptosisberberinedna methyltransferasehepatocellular carcinomap53 |
| spellingShingle | Dae-Yeon Kim Seon-Hyoung Kim Hee-Tae Cheong Chang-Six Ra Ki-Jong Rhee Bae Dong Jung Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells Korean Journal of Clinical Laboratory Science apoptosis berberine dna methyltransferase hepatocellular carcinoma p53 |
| title | Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells |
| title_full | Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells |
| title_fullStr | Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells |
| title_full_unstemmed | Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells |
| title_short | Berberine Induces p53-Dependent Apoptosis through Inhibition of DNA Methyltransferase3b in Hep3B Cells |
| title_sort | berberine induces p53 dependent apoptosis through inhibition of dna methyltransferase3b in hep3b cells |
| topic | apoptosis berberine dna methyltransferase hepatocellular carcinoma p53 |
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