| Summary: | Sustanon (SUS) consists of testosterone propionate (TP), testosterone phenylpropionate (TPP), testosterone isocaproate (TIC) and testosterone decanoate (TD) which is generally used as a medication to treat the testosterone deficiency in men. Misuse of SUS to enhance the ability of the athletes can be a very serious risk for their health which is discouraged. Therefore, the main aim of this work was based on developing a novel biosensing system for determination of different types of testosterones in SUS to monitor it. A rotating glassy carbon electrode (GCE) was modified with fullerene C60-multiwalled carbon nanotubes-ionic liquid (C60-MWCNTs-IL) and used as the working electrode for recording second-order hydrodynamic square wave voltammetric (hydrodynamic SWV) data at different step potentials including 0.02, 0.025, 0.03, 0.035, 0.04 and 0.045 V as a sensitive method. Different types of testosterones were extracted and preconcentrated by quadruple templates molecularly imprinted polymers (QTMIPs) and they were determined by C60-MWCNTs-IL/GCE. The testosterones showed severely overlapped peaks at the surface of C60-MWCNTs-IL/GCE which were resolved by three-way calibration based on second-order hydrodynamic SWV data. Therefore, a novel sensor assisted by QTMIPs and second-order calibration methods was developed which had a very good performance for the analysis of synthetic and real matrices in simultaneous determination of TP, TPP, TIC and TD with aim of monitoring the SUS.
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