Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia
Introduction: Pseudomonas aeruginosa is one of the major bacterial nosocomial infections. Metallo-β-lactamases (MBLs) are one of the most important agents for resistance against carbapenem. Based on the role of carbapenem for Pseudomonas infection treatment, in this research clinical strains of MBL...
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| Format: | Article |
| Language: | English |
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Islamic Azad University
2015-03-01
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| Series: | Report of Health Care |
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| Online Access: | http://jrhc.miau.ac.ir/article_2636_11cdeabde5aa309e925fdc6ed8c8153b.pdf |
| _version_ | 1818827562762108928 |
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| author | Ali Amin Galvani Amir Tukmechi |
| author_facet | Ali Amin Galvani Amir Tukmechi |
| author_sort | Ali Amin Galvani |
| collection | DOAJ |
| description | Introduction: Pseudomonas aeruginosa is one of the major bacterial nosocomial infections. Metallo-β-lactamases (MBLs) are one of the most important agents for resistance against carbapenem. Based on the role of carbapenem for Pseudomonas infection treatment, in this research clinical strains of MBL producing bacteria were studied.<br />Methods: Fifty Pseudomonas aeruginosa isolated from clinical samples were collected from patients that referred to the Emam Khomeini and Mottahari hospitals of Urmia. After bacterial confirmation with standard bacteriologic tests, bacterial sensitivity was assayed against ten common antibiotics by the method of disk diffusion test. Then imipenem-resistant strains were candidate for the identification of MBL production by imipenem-EDTA (Ethylenediamine<br />tetra acetic acid ) combination disk test.<br />Results: Of all 50 isolated Pseudomonas aeruginosa, 36 isolates (72%) were resistant against imipenem. Obtained results from imipenem-EDTA combined disk test showed that 32.36 isolates (88.9%) had MBL.<br />Conclusion: Although Pseudomonas aeruginosa producing MBL are resistant against all of β-lactams antibiotics, but determining the strains that produce MBL can help physicians to select the suitable antibiotic for treatment and improve the prognosis of the infection due to this bacterium. |
| first_indexed | 2024-12-19T00:45:32Z |
| format | Article |
| id | doaj.art-64ce953db6494889b6a74c6a21b7080a |
| institution | Directory Open Access Journal |
| issn | 2423-4141 2423-4141 |
| language | English |
| last_indexed | 2024-12-19T00:45:32Z |
| publishDate | 2015-03-01 |
| publisher | Islamic Azad University |
| record_format | Article |
| series | Report of Health Care |
| spelling | doaj.art-64ce953db6494889b6a74c6a21b7080a2022-12-21T20:44:16ZengIslamic Azad UniversityReport of Health Care2423-41412423-41412015-03-011265582636Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of UrmiaAli Amin Galvani0Amir Tukmechi1MSc, Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, IranAssistant Professor, Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, IranIntroduction: Pseudomonas aeruginosa is one of the major bacterial nosocomial infections. Metallo-β-lactamases (MBLs) are one of the most important agents for resistance against carbapenem. Based on the role of carbapenem for Pseudomonas infection treatment, in this research clinical strains of MBL producing bacteria were studied.<br />Methods: Fifty Pseudomonas aeruginosa isolated from clinical samples were collected from patients that referred to the Emam Khomeini and Mottahari hospitals of Urmia. After bacterial confirmation with standard bacteriologic tests, bacterial sensitivity was assayed against ten common antibiotics by the method of disk diffusion test. Then imipenem-resistant strains were candidate for the identification of MBL production by imipenem-EDTA (Ethylenediamine<br />tetra acetic acid ) combination disk test.<br />Results: Of all 50 isolated Pseudomonas aeruginosa, 36 isolates (72%) were resistant against imipenem. Obtained results from imipenem-EDTA combined disk test showed that 32.36 isolates (88.9%) had MBL.<br />Conclusion: Although Pseudomonas aeruginosa producing MBL are resistant against all of β-lactams antibiotics, but determining the strains that produce MBL can help physicians to select the suitable antibiotic for treatment and improve the prognosis of the infection due to this bacterium.http://jrhc.miau.ac.ir/article_2636_11cdeabde5aa309e925fdc6ed8c8153b.pdfImipenem-EDTA combination disk testMetallo-β-lactamasePseudomonas aeruginosaUrmia |
| spellingShingle | Ali Amin Galvani Amir Tukmechi Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia Report of Health Care Imipenem-EDTA combination disk test Metallo-β-lactamase Pseudomonas aeruginosa Urmia |
| title | Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia |
| title_full | Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia |
| title_fullStr | Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia |
| title_full_unstemmed | Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia |
| title_short | Determination of the prevalence of metallo-β-lactamases producing Pseudomonas aeruginosa strains from clinical samples by imipenem-EDTA combination disk method in Mottahari and Emam Khomaini hospitals of Urmia |
| title_sort | determination of the prevalence of metallo β lactamases producing pseudomonas aeruginosa strains from clinical samples by imipenem edta combination disk method in mottahari and emam khomaini hospitals of urmia |
| topic | Imipenem-EDTA combination disk test Metallo-β-lactamase Pseudomonas aeruginosa Urmia |
| url | http://jrhc.miau.ac.ir/article_2636_11cdeabde5aa309e925fdc6ed8c8153b.pdf |
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