Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans
Diaphragma juglandis Fructus (DJF) is rich in biologically active phytochemicals, exhibiting a wide range of biological activities. According to the results of antioxidant experiments in vitro, four compounds were isolated by chromatography and identified by NMR. (1′-methyl-2′-hydroxy) propane-O-α-D...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621000712 |
| _version_ | 1819095317600010240 |
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| author | Qianqian Hong Shuxiang Geng Jian Ji Yongli Ye Deping Xu Yinzhi Zhang Xiulan Sun |
| author_facet | Qianqian Hong Shuxiang Geng Jian Ji Yongli Ye Deping Xu Yinzhi Zhang Xiulan Sun |
| author_sort | Qianqian Hong |
| collection | DOAJ |
| description | Diaphragma juglandis Fructus (DJF) is rich in biologically active phytochemicals, exhibiting a wide range of biological activities. According to the results of antioxidant experiments in vitro, four compounds were isolated by chromatography and identified by NMR. (1′-methyl-2′-hydroxy) propane-O-α-D-glucopyranoside (1), (4′-hydroxyphenyl) methylene-O-β-D-glucopyranosyl-(4 → 1)-α-L-arabinopyranoside (2), 2-carboxy-5,7-dihydroxy-3-naphthyl-β-D-glucopyranoside (4) (CDG), neither of which have been isolated from DJF before. CDG exhibited the highest antioxidant capacity in vitro so that we used C. elegans to evaluate antioxidant capacity and examined the changes in metabolome by GC-TOF/MS. Our study showed that CDG could improve oxidative stress and prolong lifespan in C. elegans by improving the activities of antioxidant enzymes and reducing ROS and MDA content. Metabolomics analysis revealed that CDG affected various metabolic pathways (amino acid, carbohydrate, lipid metabolism) and further affected TCA cycle and energy status, thus reduced cellular oxidative stress. These results suggest that DJF could be a potential source of natural antioxidants, specifically, CDG could be used as an antioxidant ingredient in food and pharmaceutical industries. |
| first_indexed | 2024-12-21T23:41:23Z |
| format | Article |
| id | doaj.art-64d48ffb50f54d58a525d12f28ac12cf |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T23:41:23Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-64d48ffb50f54d58a525d12f28ac12cf2022-12-21T18:46:13ZengElsevierJournal of Functional Foods1756-46462021-05-0180104422Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegansQianqian Hong0Shuxiang Geng1Jian Ji2Yongli Ye3Deping Xu4Yinzhi Zhang5Xiulan Sun6State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR ChinaYunnan Academy of Forestry, Kunming, Yunnan 650201, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Corresponding author.Diaphragma juglandis Fructus (DJF) is rich in biologically active phytochemicals, exhibiting a wide range of biological activities. According to the results of antioxidant experiments in vitro, four compounds were isolated by chromatography and identified by NMR. (1′-methyl-2′-hydroxy) propane-O-α-D-glucopyranoside (1), (4′-hydroxyphenyl) methylene-O-β-D-glucopyranosyl-(4 → 1)-α-L-arabinopyranoside (2), 2-carboxy-5,7-dihydroxy-3-naphthyl-β-D-glucopyranoside (4) (CDG), neither of which have been isolated from DJF before. CDG exhibited the highest antioxidant capacity in vitro so that we used C. elegans to evaluate antioxidant capacity and examined the changes in metabolome by GC-TOF/MS. Our study showed that CDG could improve oxidative stress and prolong lifespan in C. elegans by improving the activities of antioxidant enzymes and reducing ROS and MDA content. Metabolomics analysis revealed that CDG affected various metabolic pathways (amino acid, carbohydrate, lipid metabolism) and further affected TCA cycle and energy status, thus reduced cellular oxidative stress. These results suggest that DJF could be a potential source of natural antioxidants, specifically, CDG could be used as an antioxidant ingredient in food and pharmaceutical industries.http://www.sciencedirect.com/science/article/pii/S1756464621000712Diaphragma juglandis FructusAntioxidant activity2-carboxy-5,7-dihydroxy-3-naphthyl-β-D-glucopyranosideCaenorhabditis elegansMetabolomicsGC-TOF/MS |
| spellingShingle | Qianqian Hong Shuxiang Geng Jian Ji Yongli Ye Deping Xu Yinzhi Zhang Xiulan Sun Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans Journal of Functional Foods Diaphragma juglandis Fructus Antioxidant activity 2-carboxy-5,7-dihydroxy-3-naphthyl-β-D-glucopyranoside Caenorhabditis elegans Metabolomics GC-TOF/MS |
| title | Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans |
| title_full | Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans |
| title_fullStr | Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans |
| title_full_unstemmed | Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans |
| title_short | Separation and identification of antioxidant chemical components in Diaphragma juglandis Fructus and functional evaluation in Caenorhabditis elegans |
| title_sort | separation and identification of antioxidant chemical components in diaphragma juglandis fructus and functional evaluation in caenorhabditis elegans |
| topic | Diaphragma juglandis Fructus Antioxidant activity 2-carboxy-5,7-dihydroxy-3-naphthyl-β-D-glucopyranoside Caenorhabditis elegans Metabolomics GC-TOF/MS |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621000712 |
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