Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route
Dengue virus, the causative agent of dengue fever, life-threatening hemorrhagic fever, and shock syndrome, is mainly transmitted to humans through mosquito vectors. It can also be transmitted through atypical routes, including needle stick injury, vertical transmission, blood transfusion, and organ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1394 |
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| author | Minyue Qiu Lixin Zhao Junjie Zhang Yalan Wang Minchi Liu Dong Hua Xiaoyan Ding Xiaoyang Zhou Jie Zeng Huacheng Yan Jintao Li |
| author_facet | Minyue Qiu Lixin Zhao Junjie Zhang Yalan Wang Minchi Liu Dong Hua Xiaoyan Ding Xiaoyang Zhou Jie Zeng Huacheng Yan Jintao Li |
| author_sort | Minyue Qiu |
| collection | DOAJ |
| description | Dengue virus, the causative agent of dengue fever, life-threatening hemorrhagic fever, and shock syndrome, is mainly transmitted to humans through mosquito vectors. It can also be transmitted through atypical routes, including needle stick injury, vertical transmission, blood transfusion, and organ transplantation. In addition, sporadic cases which have no clear infectious causes have raised the respiratory exposure concerns, and the risks remain unclear. Here, we analyze the respiratory infectivity of the dengue virus in BALB/c suckling and adult immunodeficient mice by the intranasal inoculation of dengue virus serotype 2. The infected mice presented with clinical symptoms, including excitement, emaciation, malaise, and death. Viremia was detected for 3 days post inoculation. Histopathological changes were observed in the brain, liver, and spleen. The virus showed evident brain tropism post inoculation and viral loads peaked at 7 days post inoculation. Furthermore, the virus was isolated from the infected mice; the sequence homology between the origin and isolates was 99.99%. Similar results were observed in adult IFN-α/β receptor-deficient mice. Overall, dengue virus can infect suckling mice and adult immune-deficient mice via the nasal route. This study broadens our perception of atypical dengue transmission routes and provides evidence of nasal transmission of dengue virus in the absence of mosquito vectors. |
| first_indexed | 2024-03-09T05:44:07Z |
| format | Article |
| id | doaj.art-64d595a2acce4a38bf08ded50879b7f2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:44:07Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-64d595a2acce4a38bf08ded50879b7f22023-12-03T12:22:49ZengMDPI AGViruses1999-49152022-06-01147139410.3390/v14071394Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal RouteMinyue Qiu0Lixin Zhao1Junjie Zhang2Yalan Wang3Minchi Liu4Dong Hua5Xiaoyan Ding6Xiaoyang Zhou7Jie Zeng8Huacheng Yan9Jintao Li10Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaCenter for Disease Control and Prevention of Southern Military Theatre, Guangzhou 510507, ChinaDepartment of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, ChinaDengue virus, the causative agent of dengue fever, life-threatening hemorrhagic fever, and shock syndrome, is mainly transmitted to humans through mosquito vectors. It can also be transmitted through atypical routes, including needle stick injury, vertical transmission, blood transfusion, and organ transplantation. In addition, sporadic cases which have no clear infectious causes have raised the respiratory exposure concerns, and the risks remain unclear. Here, we analyze the respiratory infectivity of the dengue virus in BALB/c suckling and adult immunodeficient mice by the intranasal inoculation of dengue virus serotype 2. The infected mice presented with clinical symptoms, including excitement, emaciation, malaise, and death. Viremia was detected for 3 days post inoculation. Histopathological changes were observed in the brain, liver, and spleen. The virus showed evident brain tropism post inoculation and viral loads peaked at 7 days post inoculation. Furthermore, the virus was isolated from the infected mice; the sequence homology between the origin and isolates was 99.99%. Similar results were observed in adult IFN-α/β receptor-deficient mice. Overall, dengue virus can infect suckling mice and adult immune-deficient mice via the nasal route. This study broadens our perception of atypical dengue transmission routes and provides evidence of nasal transmission of dengue virus in the absence of mosquito vectors.https://www.mdpi.com/1999-4915/14/7/1394dengue virusintranasal routeneonate miceA6 miceDENV-2 |
| spellingShingle | Minyue Qiu Lixin Zhao Junjie Zhang Yalan Wang Minchi Liu Dong Hua Xiaoyan Ding Xiaoyang Zhou Jie Zeng Huacheng Yan Jintao Li Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route Viruses dengue virus intranasal route neonate mice A6 mice DENV-2 |
| title | Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route |
| title_full | Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route |
| title_fullStr | Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route |
| title_full_unstemmed | Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route |
| title_short | Effective Infection with Dengue Virus in Experimental Neonate and Adult Mice through the Intranasal Route |
| title_sort | effective infection with dengue virus in experimental neonate and adult mice through the intranasal route |
| topic | dengue virus intranasal route neonate mice A6 mice DENV-2 |
| url | https://www.mdpi.com/1999-4915/14/7/1394 |
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