Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair
Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423002120 |
| _version_ | 1797682463970951168 |
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| author | Lin Huang Wantao Wang Yiwen Xian Lei Liu Jinghao Fan Hongmei Liu Zhaomin Zheng Decheng Wu |
| author_facet | Lin Huang Wantao Wang Yiwen Xian Lei Liu Jinghao Fan Hongmei Liu Zhaomin Zheng Decheng Wu |
| author_sort | Lin Huang |
| collection | DOAJ |
| description | Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) through integrating photo-crosslink of methacrylate chitosan (CSMA) with Schiff base reaction between CSMA and aldehyde polyethylene glycol (PEGDA). The CSMA-PEGDA-L possessed a stronger compressive strength than the photo-crosslinked CSMA-L hydrogel and Schiff-base-crosslinked CSMA-PEGDA hydrogel. This chitosan/PEG hydrogel showed low cytotoxicity from incubation experiments of nucleus pulpous cells. When implanted on the punctured IVD of rat's tail, the CSMA-PEGDA-L hydrogel could well retard the progression of IVD degeneration through physical plugging, powerfully proven by radiological and histological evaluations. This work demonstrated the strategy of in situ injectable glue may be a potential solution for prevention of IVD degeneration. |
| first_indexed | 2024-03-12T00:01:04Z |
| format | Article |
| id | doaj.art-64d889bc672a4890bad4b0d0bfa7ae0a |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-12T00:01:04Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-64d889bc672a4890bad4b0d0bfa7ae0a2023-09-18T04:30:16ZengElsevierMaterials Today Bio2590-00642023-10-0122100752Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repairLin Huang0Wantao Wang1Yiwen Xian2Lei Liu3Jinghao Fan4Hongmei Liu5Zhaomin Zheng6Decheng Wu7Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, ChinaGuangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; Department of Spine Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510275, ChinaGuangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, ChinaGuangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, ChinaGuangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, ChinaGuangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; Corresponding author.Department of Spine Surgery, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510275, China; Corresponding author.Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; Corresponding author.Intervertebral disc (IVD) degeneration occurred with the increasing age or accidents has puzzled peoples in daily life. To seal IVD defect by injectable hydrogels is a promising method for slowing down IVD degeneration. Herein, we reported a rapidly in situ forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) through integrating photo-crosslink of methacrylate chitosan (CSMA) with Schiff base reaction between CSMA and aldehyde polyethylene glycol (PEGDA). The CSMA-PEGDA-L possessed a stronger compressive strength than the photo-crosslinked CSMA-L hydrogel and Schiff-base-crosslinked CSMA-PEGDA hydrogel. This chitosan/PEG hydrogel showed low cytotoxicity from incubation experiments of nucleus pulpous cells. When implanted on the punctured IVD of rat's tail, the CSMA-PEGDA-L hydrogel could well retard the progression of IVD degeneration through physical plugging, powerfully proven by radiological and histological evaluations. This work demonstrated the strategy of in situ injectable glue may be a potential solution for prevention of IVD degeneration.http://www.sciencedirect.com/science/article/pii/S2590006423002120ChitosanPEGInjectable hydrogelTissue engineeringIntervertebral disc repair |
| spellingShingle | Lin Huang Wantao Wang Yiwen Xian Lei Liu Jinghao Fan Hongmei Liu Zhaomin Zheng Decheng Wu Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair Materials Today Bio Chitosan PEG Injectable hydrogel Tissue engineering Intervertebral disc repair |
| title | Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair |
| title_full | Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair |
| title_fullStr | Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair |
| title_full_unstemmed | Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair |
| title_short | Rapidly in situ forming an injectable Chitosan/PEG hydrogel for intervertebral disc repair |
| title_sort | rapidly in situ forming an injectable chitosan peg hydrogel for intervertebral disc repair |
| topic | Chitosan PEG Injectable hydrogel Tissue engineering Intervertebral disc repair |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423002120 |
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