P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS
Objectives: Vascular dysfunction is common in type 2 diabetes and obesity. The functional role of hyperinsulinaemia on human blood vessels in obese individuals remains unclear. We hypothesised that in young women, change in vessel function in a hyperinsulinaemic milieu would be influenced more by ad...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-12-01
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| Series: | Artery Research |
| Online Access: | https://www.atlantis-press.com/article/125927294/view |
| _version_ | 1811300933459509248 |
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| author | M. Banerjee C. Austin V. Charlton-Menys W.F. Fraser P. Pemberton R.A. Malik J.K. Cruickshank |
| author_facet | M. Banerjee C. Austin V. Charlton-Menys W.F. Fraser P. Pemberton R.A. Malik J.K. Cruickshank |
| author_sort | M. Banerjee |
| collection | DOAJ |
| description | Objectives: Vascular dysfunction is common in type 2 diabetes and obesity. The functional role of hyperinsulinaemia on human blood vessels in obese individuals remains unclear. We hypothesised that in young women, change in vessel function in a hyperinsulinaemic milieu would be influenced more by adiposity than plasma glucose.
Methods and Results: Women in a pregnancy cohort were stratified into upper & lower quartiles of fasting plasma glucose (FPG) when seen at follow-up 20 months after delivery. After subcutaneous biopsy, small arteries were tested ex-vivo by wire myography for vasoconstrictor [Noradrenaline (NA)] and vasodilator [carbachol and sodium nitroprusside (SNP)] responses before and after incubation with 100 mU/ml human insulin.
Results: Women with higher FPG had attenuated NA-contractile responses [0.8 (0.4–1.39) vs. 0.6 (−0.5 to 1.7) mN/mm, p=0.011), but differences in maximum response to carbachol (ΔEDDmax) before and after insulin incubation did not increase [26.8 (4.8–48.7) vs. 18.5 (−3.3 to 30.2) %, p=0.55) compared with those with lower FPG. Insulin reduced NA-induced contraction in those with higher [3.5 (2.4–4.6) vs. 2.4 (1.4–3.4) mN/mm: p=0.004] but not in those with lower BMI [4.1 (2.8–5.3) vs. 3.7 (2.5–5.0) mN/mm: p=0.33]. ΔEDDmax was greater in the high than low BMI group [37.7 (18.0 to 57.3) % vs.6.3 (−6.5 to 19.1) %, p=0.007].
Conclusions: Small arteries from women with greater adiposity and fasting glucose, exhibited reduced contraction with NA but only the former showed improved EDD, when tested in a hyperinsulinaemic milieu. Hyperinsulinaemia may be important in maintaining endothelial function in obesity. |
| first_indexed | 2024-04-13T06:59:44Z |
| format | Article |
| id | doaj.art-64df95179e544d9ca5f3f6dd6f8fd021 |
| institution | Directory Open Access Journal |
| issn | 1876-4401 |
| language | English |
| last_indexed | 2024-04-13T06:59:44Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Artery Research |
| spelling | doaj.art-64df95179e544d9ca5f3f6dd6f8fd0212022-12-22T02:57:09ZengBMCArtery Research1876-44012009-12-013410.1016/j.artres.2009.10.028P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLSM. BanerjeeC. AustinV. Charlton-MenysW.F. FraserP. PembertonR.A. MalikJ.K. CruickshankObjectives: Vascular dysfunction is common in type 2 diabetes and obesity. The functional role of hyperinsulinaemia on human blood vessels in obese individuals remains unclear. We hypothesised that in young women, change in vessel function in a hyperinsulinaemic milieu would be influenced more by adiposity than plasma glucose. Methods and Results: Women in a pregnancy cohort were stratified into upper & lower quartiles of fasting plasma glucose (FPG) when seen at follow-up 20 months after delivery. After subcutaneous biopsy, small arteries were tested ex-vivo by wire myography for vasoconstrictor [Noradrenaline (NA)] and vasodilator [carbachol and sodium nitroprusside (SNP)] responses before and after incubation with 100 mU/ml human insulin. Results: Women with higher FPG had attenuated NA-contractile responses [0.8 (0.4–1.39) vs. 0.6 (−0.5 to 1.7) mN/mm, p=0.011), but differences in maximum response to carbachol (ΔEDDmax) before and after insulin incubation did not increase [26.8 (4.8–48.7) vs. 18.5 (−3.3 to 30.2) %, p=0.55) compared with those with lower FPG. Insulin reduced NA-induced contraction in those with higher [3.5 (2.4–4.6) vs. 2.4 (1.4–3.4) mN/mm: p=0.004] but not in those with lower BMI [4.1 (2.8–5.3) vs. 3.7 (2.5–5.0) mN/mm: p=0.33]. ΔEDDmax was greater in the high than low BMI group [37.7 (18.0 to 57.3) % vs.6.3 (−6.5 to 19.1) %, p=0.007]. Conclusions: Small arteries from women with greater adiposity and fasting glucose, exhibited reduced contraction with NA but only the former showed improved EDD, when tested in a hyperinsulinaemic milieu. Hyperinsulinaemia may be important in maintaining endothelial function in obesity.https://www.atlantis-press.com/article/125927294/view |
| spellingShingle | M. Banerjee C. Austin V. Charlton-Menys W.F. Fraser P. Pemberton R.A. Malik J.K. Cruickshank P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS Artery Research |
| title | P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS |
| title_full | P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS |
| title_fullStr | P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS |
| title_full_unstemmed | P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS |
| title_short | P2.12 THE ROLE OF INSULIN ON FUNCTION OF RESISTANCE ARTERIES FROM OBESE YOUNG WOMEN AT RISK OF ‘DIABETES’ AND CONTROLS |
| title_sort | p2 12 the role of insulin on function of resistance arteries from obese young women at risk of diabetes and controls |
| url | https://www.atlantis-press.com/article/125927294/view |
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