The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia
The anti-inflammatory effects of the CRG/Ech complex in LPS-induced endotoxemia were investigated in vivo in mice and in vitro in LPS-stimulated RAW 264.7 cells and peritoneal macrophages. The results indicated that the CRG/Ech complex suppressed the LPS-induced inflammatory response by reducing the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11702 |
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| author | Irina M. Yermak Anna O. Kravchenko Eleonora I. Khasina Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina V. Sokolova Galina N. Likhatskaya Dmitry L. Aminin |
| author_facet | Irina M. Yermak Anna O. Kravchenko Eleonora I. Khasina Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina V. Sokolova Galina N. Likhatskaya Dmitry L. Aminin |
| author_sort | Irina M. Yermak |
| collection | DOAJ |
| description | The anti-inflammatory effects of the CRG/Ech complex in LPS-induced endotoxemia were investigated in vivo in mice and in vitro in LPS-stimulated RAW 264.7 cells and peritoneal macrophages. The results indicated that the CRG/Ech complex suppressed the LPS-induced inflammatory response by reducing the production of ROS and NO in the macrophages. Furthermore, the in vivo experiment indicated that the CRG/Ech complex minimized disorders of the physiological and metabolic processes in mice subjected to LPS intoxication and reduced the levels of proinflammatory cytokines in the mouse serum. The preventive administration of the CRG/Ech complex to mice prevented endotoxin-induced damage in the mouse model of endotoxemia, increased the mice’s resistance to LPS, and prevented increases in the levels of proinflammatory cytokines (TNFα). In this work, we showed by the molecular docking that Ech interacted with carrageenan, and that H-donor and H-acceptor bonds are involved in the formation of the complex. |
| first_indexed | 2024-03-09T21:38:11Z |
| format | Article |
| id | doaj.art-64dfb38c7fa4425683ec5499383c8447 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:38:11Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-64dfb38c7fa4425683ec5499383c84472023-11-23T20:37:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191170210.3390/ijms231911702The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental EndotoxemiaIrina M. Yermak0Anna O. Kravchenko1Eleonora I. Khasina2Ekaterina S. Menchinskaya3Evgeny A. Pislyagin4Ekaterina V. Sokolova5Galina N. Likhatskaya6Dmitry L. Aminin7G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, RussiaThe anti-inflammatory effects of the CRG/Ech complex in LPS-induced endotoxemia were investigated in vivo in mice and in vitro in LPS-stimulated RAW 264.7 cells and peritoneal macrophages. The results indicated that the CRG/Ech complex suppressed the LPS-induced inflammatory response by reducing the production of ROS and NO in the macrophages. Furthermore, the in vivo experiment indicated that the CRG/Ech complex minimized disorders of the physiological and metabolic processes in mice subjected to LPS intoxication and reduced the levels of proinflammatory cytokines in the mouse serum. The preventive administration of the CRG/Ech complex to mice prevented endotoxin-induced damage in the mouse model of endotoxemia, increased the mice’s resistance to LPS, and prevented increases in the levels of proinflammatory cytokines (TNFα). In this work, we showed by the molecular docking that Ech interacted with carrageenan, and that H-donor and H-acceptor bonds are involved in the formation of the complex.https://www.mdpi.com/1422-0067/23/19/11702lipopolisaccharideendotoxinemiacarrageenanechinochromecomplexROS |
| spellingShingle | Irina M. Yermak Anna O. Kravchenko Eleonora I. Khasina Ekaterina S. Menchinskaya Evgeny A. Pislyagin Ekaterina V. Sokolova Galina N. Likhatskaya Dmitry L. Aminin The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia International Journal of Molecular Sciences lipopolisaccharide endotoxinemia carrageenan echinochrome complex ROS |
| title | The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia |
| title_full | The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia |
| title_fullStr | The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia |
| title_full_unstemmed | The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia |
| title_short | The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia |
| title_sort | anti inflammatory effect of carrageenan echinochrom complex at experimental endotoxemia |
| topic | lipopolisaccharide endotoxinemia carrageenan echinochrome complex ROS |
| url | https://www.mdpi.com/1422-0067/23/19/11702 |
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