Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants
The involvement of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in countermanding heat-inhibited photosynthetic features were studied in wheat (<i>Triticum aestivum</i> L.). Heat stress (HS) was employed at 40 °C after establishment for 6 h daily, and then plants w...
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MDPI AG
2021-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/1/108 |
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| author | Noushina Iqbal Shahid Umar Nafees A. Khan Francisco J. Corpas |
| author_facet | Noushina Iqbal Shahid Umar Nafees A. Khan Francisco J. Corpas |
| author_sort | Noushina Iqbal |
| collection | DOAJ |
| description | The involvement of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in countermanding heat-inhibited photosynthetic features were studied in wheat (<i>Triticum aestivum</i> L.). Heat stress (HS) was employed at 40 °C after establishment for 6 h daily, and then plants were allowed to recover at 25 °C and grown for 30 days. Glucose (Glc) content increased under HS and repressed plant photosynthetic ability, but the application of sodium nitroprusside (SNP, as NO donor) either alone or with sodium hydrosulfide (NaHS, as H<sub>2</sub>S donor) reduced Glc-mediated photosynthetic suppression by enhancing ascorbate-glutathione (AsA-GSH) metabolism and antioxidant system, which reduced oxidative stress with decreased H<sub>2</sub>O<sub>2</sub> and TBARS content. Oxidative stress reduction or inhibiting Glc repression was maximum with combined SNP and NaHS treatment, which was substantiated by 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and hypotaurine (HT), scavengers for NO and H<sub>2</sub>S, respectively. The scavenge of H<sub>2</sub>S reduced NO-mediated alleviation of HS suggesting of its downstream action in NO-mediated heat-tolerance. However, a simultaneous decrease of both (NO and H<sub>2</sub>S) led to higher Glc-mediated repression of photosynthesis and oxidative stress in terms of increased H<sub>2</sub>O<sub>2</sub> content that was comparable to HS plants. Thus, NO and H<sub>2</sub>S cooperate to enhance photosynthesis under HS by reducing H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and excess Glc-mediated photosynthetic suppression. |
| first_indexed | 2024-03-09T04:53:02Z |
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| language | English |
| last_indexed | 2024-03-09T04:53:02Z |
| publishDate | 2021-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-64dfbc8f28ce4afa8fb7286d0e4db6312023-12-03T13:08:39ZengMDPI AGAntioxidants2076-39212021-01-0110110810.3390/antiox10010108Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) PlantsNoushina Iqbal0Shahid Umar1Nafees A. Khan2Francisco J. Corpas3Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaDepartment of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, IndiaPlant Physiology and Biochemistry Laboratory, Department of Botany, Aligarh Muslim University, Aligarh 202002, IndiaGroup of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, SpainThe involvement of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in countermanding heat-inhibited photosynthetic features were studied in wheat (<i>Triticum aestivum</i> L.). Heat stress (HS) was employed at 40 °C after establishment for 6 h daily, and then plants were allowed to recover at 25 °C and grown for 30 days. Glucose (Glc) content increased under HS and repressed plant photosynthetic ability, but the application of sodium nitroprusside (SNP, as NO donor) either alone or with sodium hydrosulfide (NaHS, as H<sub>2</sub>S donor) reduced Glc-mediated photosynthetic suppression by enhancing ascorbate-glutathione (AsA-GSH) metabolism and antioxidant system, which reduced oxidative stress with decreased H<sub>2</sub>O<sub>2</sub> and TBARS content. Oxidative stress reduction or inhibiting Glc repression was maximum with combined SNP and NaHS treatment, which was substantiated by 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and hypotaurine (HT), scavengers for NO and H<sub>2</sub>S, respectively. The scavenge of H<sub>2</sub>S reduced NO-mediated alleviation of HS suggesting of its downstream action in NO-mediated heat-tolerance. However, a simultaneous decrease of both (NO and H<sub>2</sub>S) led to higher Glc-mediated repression of photosynthesis and oxidative stress in terms of increased H<sub>2</sub>O<sub>2</sub> content that was comparable to HS plants. Thus, NO and H<sub>2</sub>S cooperate to enhance photosynthesis under HS by reducing H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and excess Glc-mediated photosynthetic suppression.https://www.mdpi.com/2076-3921/10/1/108AsA-GSHglucoseH<sub>2</sub>Snitric oxidephotosynthesis |
| spellingShingle | Noushina Iqbal Shahid Umar Nafees A. Khan Francisco J. Corpas Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants Antioxidants AsA-GSH glucose H<sub>2</sub>S nitric oxide photosynthesis |
| title | Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants |
| title_full | Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants |
| title_fullStr | Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants |
| title_full_unstemmed | Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants |
| title_short | Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (<i>Triticum aestivum</i> L.) Plants |
| title_sort | nitric oxide and hydrogen sulfide coordinately reduce glucose sensitivity and decrease oxidative stress via ascorbate glutathione cycle in heat stressed wheat i triticum aestivum i l plants |
| topic | AsA-GSH glucose H<sub>2</sub>S nitric oxide photosynthesis |
| url | https://www.mdpi.com/2076-3921/10/1/108 |
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