Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion
Background: Na + /Ca 2+ exchanger (NCX) plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats wi...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2015-01-01
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| Series: | Chinese Medical Journal |
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| Online Access: | http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=22;spage=3083;epage=3087;aulast=Sun |
| _version_ | 1819264118056550400 |
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| author | Dan Sun Jun-Hua Xiao Yan Bai Mo-Si Chen Jia-Sheng Hu Ge-Fei Wu Bing Mao Shu-Hua Wu Yan Hu |
| author_facet | Dan Sun Jun-Hua Xiao Yan Bai Mo-Si Chen Jia-Sheng Hu Ge-Fei Wu Bing Mao Shu-Hua Wu Yan Hu |
| author_sort | Dan Sun |
| collection | DOAJ |
| description | Background: Na + /Ca 2+ exchanger (NCX) plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats with hyperthermia-induced convulsion.
Methods: Twenty-one Sprague Dawley rats were randomly assigned to control group, convulsion-prone group and convulsion-resistant group (n = 7 in each group). Whole-cell patch-clamp method was used to record NCX currents. Both the Western blotting analysis and immunofluorescence labeling techniques were used to examine the expression of NCX3.
Results: NCX currents were decreased in rats after febrile convulsion. Compared to the control group, NCX3 expression was decreased by about 40% and 50% in the hippocampus and cerebrocortex of convulsion-prone rats, respectively. Furthermore, the extent of reduction in NCX3 expression seemed to correlate with the number of seizures.
Conclusions: There is a significant reduction in NCX3 expression in rats with febrile convulsions. Our findings also indicate a potential link between NCX3 expression, febrile convulsion in early childhood, and adult onset of epilepsy. |
| first_indexed | 2024-12-23T20:24:24Z |
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| institution | Directory Open Access Journal |
| issn | 0366-6999 |
| language | English |
| last_indexed | 2024-12-23T20:24:24Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-64e048a5f60248419cc66534d0a868e42022-12-21T17:32:26ZengWolters KluwerChinese Medical Journal0366-69992015-01-01128223083308710.4103/0366-6999.169103Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced ConvulsionDan SunJun-Hua XiaoYan BaiMo-Si ChenJia-Sheng HuGe-Fei WuBing MaoShu-Hua WuYan HuBackground: Na + /Ca 2+ exchanger (NCX) plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats with hyperthermia-induced convulsion. Methods: Twenty-one Sprague Dawley rats were randomly assigned to control group, convulsion-prone group and convulsion-resistant group (n = 7 in each group). Whole-cell patch-clamp method was used to record NCX currents. Both the Western blotting analysis and immunofluorescence labeling techniques were used to examine the expression of NCX3. Results: NCX currents were decreased in rats after febrile convulsion. Compared to the control group, NCX3 expression was decreased by about 40% and 50% in the hippocampus and cerebrocortex of convulsion-prone rats, respectively. Furthermore, the extent of reduction in NCX3 expression seemed to correlate with the number of seizures. Conclusions: There is a significant reduction in NCX3 expression in rats with febrile convulsions. Our findings also indicate a potential link between NCX3 expression, febrile convulsion in early childhood, and adult onset of epilepsy.http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=22;spage=3083;epage=3087;aulast=SunCerebrocortex; Febrile Convulsion; Hippocampus; Hyperthermia; Na + /Ca 2+ Exchanger 3 |
| spellingShingle | Dan Sun Jun-Hua Xiao Yan Bai Mo-Si Chen Jia-Sheng Hu Ge-Fei Wu Bing Mao Shu-Hua Wu Yan Hu Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion Chinese Medical Journal Cerebrocortex; Febrile Convulsion; Hippocampus; Hyperthermia; Na + /Ca 2+ Exchanger 3 |
| title | Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion |
| title_full | Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion |
| title_fullStr | Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion |
| title_full_unstemmed | Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion |
| title_short | Na + /Ca 2+ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion |
| title_sort | na ca 2 exchanger 3 is downregulated in the hippocampus and cerebrocortex of rats with hyperthermia induced convulsion |
| topic | Cerebrocortex; Febrile Convulsion; Hippocampus; Hyperthermia; Na + /Ca 2+ Exchanger 3 |
| url | http://www.cmj.org/article.asp?issn=0366-6999;year=2015;volume=128;issue=22;spage=3083;epage=3087;aulast=Sun |
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