Primer extension mutagenesis powered by selective rolling circle amplification.

Primer extension mutagenesis powered by selective rolling circle amplification.

Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosyla...

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Bibliographic Details
Main Authors:	Tuomas Huovinen, Eeva-Christine Brockmann, Sultana Akter, Susan Perez-Gamarra, Jani Ylä-Pelto, Yuan Liu, Urpo Lamminmäki
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2012-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC3280210?pdf=render

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