Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria
Drinking water treatment plants throughout the world are increasingly facing the presence of toxic cyanobacteria in their source waters. During treatment, the oxidation of cyanobacteria changes cell morphology and can potentially lyse cells, releasing intracellular metabolites. In this study, a comb...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/5/278 |
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| author | Saber Moradinejad Caitlin M. Glover Jacinthe Mailly Tahere Zadfathollah Seighalani Sigrid Peldszus Benoit Barbeau Sarah Dorner Michèle Prévost Arash Zamyadi |
| author_facet | Saber Moradinejad Caitlin M. Glover Jacinthe Mailly Tahere Zadfathollah Seighalani Sigrid Peldszus Benoit Barbeau Sarah Dorner Michèle Prévost Arash Zamyadi |
| author_sort | Saber Moradinejad |
| collection | DOAJ |
| description | Drinking water treatment plants throughout the world are increasingly facing the presence of toxic cyanobacteria in their source waters. During treatment, the oxidation of cyanobacteria changes cell morphology and can potentially lyse cells, releasing intracellular metabolites. In this study, a combination of techniques was applied to better understand the effect of oxidation with chlorine, ozone, potassium permanganate, and hydrogen peroxide on two cell cultures (<i>Microcystis, Dolichospermum</i>) in Lake Champlain water. The discrepancy observed between flow cytometry cell viability and cell count numbers was more pronounced for hydrogen peroxide and potassium permanganate than ozone and chlorine. Liquid chromatography with organic carbon and nitrogen detection was applied to monitor the changes in dissolved organic matter fractions following oxidation. Increases in the biopolymer fraction after oxidation with chlorine and ozone were attributed to the release of intracellular algal organic matter and/or fragmentation of the cell membrane. A novel technique, Enhanced Darkfield Microscopy with Hyperspectral Imaging, was applied to chlorinated and ozonated samples. Significant changes in the peak maxima and number of peaks were observed for the cell walls post-oxidation, but this effect was muted for the cell-bound material, which remained relatively unaltered. |
| first_indexed | 2024-04-14T02:19:47Z |
| format | Article |
| id | doaj.art-64e3b1ac66a14faea0a6f74f748173b5 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T02:19:47Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-64e3b1ac66a14faea0a6f74f748173b52022-12-22T02:18:05ZengMDPI AGToxins2072-66512019-05-0111527810.3390/toxins11050278toxins11050278Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on CyanobacteriaSaber Moradinejad0Caitlin M. Glover1Jacinthe Mailly2Tahere Zadfathollah Seighalani3Sigrid Peldszus4Benoit Barbeau5Sarah Dorner6Michèle Prévost7Arash Zamyadi8BGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaDepartment of Civil & Environmental Engineering, University of Waterloo, Waterloo, ON N2L 3G1, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaBGA Innovation Hub and Civil, Mineral and Mining Engineering Department, Polytechnique Montréal, Montréal, QC H3T 1J4, CanadaDrinking water treatment plants throughout the world are increasingly facing the presence of toxic cyanobacteria in their source waters. During treatment, the oxidation of cyanobacteria changes cell morphology and can potentially lyse cells, releasing intracellular metabolites. In this study, a combination of techniques was applied to better understand the effect of oxidation with chlorine, ozone, potassium permanganate, and hydrogen peroxide on two cell cultures (<i>Microcystis, Dolichospermum</i>) in Lake Champlain water. The discrepancy observed between flow cytometry cell viability and cell count numbers was more pronounced for hydrogen peroxide and potassium permanganate than ozone and chlorine. Liquid chromatography with organic carbon and nitrogen detection was applied to monitor the changes in dissolved organic matter fractions following oxidation. Increases in the biopolymer fraction after oxidation with chlorine and ozone were attributed to the release of intracellular algal organic matter and/or fragmentation of the cell membrane. A novel technique, Enhanced Darkfield Microscopy with Hyperspectral Imaging, was applied to chlorinated and ozonated samples. Significant changes in the peak maxima and number of peaks were observed for the cell walls post-oxidation, but this effect was muted for the cell-bound material, which remained relatively unaltered.https://www.mdpi.com/2072-6651/11/5/278cyanobacteriaoxidationcell morphologyenhanced darkfield microscopy/hyperspectral imagingintracellular organic matter |
| spellingShingle | Saber Moradinejad Caitlin M. Glover Jacinthe Mailly Tahere Zadfathollah Seighalani Sigrid Peldszus Benoit Barbeau Sarah Dorner Michèle Prévost Arash Zamyadi Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria Toxins cyanobacteria oxidation cell morphology enhanced darkfield microscopy/hyperspectral imaging intracellular organic matter |
| title | Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria |
| title_full | Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria |
| title_fullStr | Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria |
| title_full_unstemmed | Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria |
| title_short | Using Advanced Spectroscopy and Organic Matter Characterization to Evaluate the Impact of Oxidation on Cyanobacteria |
| title_sort | using advanced spectroscopy and organic matter characterization to evaluate the impact of oxidation on cyanobacteria |
| topic | cyanobacteria oxidation cell morphology enhanced darkfield microscopy/hyperspectral imaging intracellular organic matter |
| url | https://www.mdpi.com/2072-6651/11/5/278 |
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