Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile
Background: After liver transplantation, HCV/HIV co-infected patients present, compared to the HCV mono-infected ones, increased HCV viral load, rapid progression to liver fibrosis and higher mortality. Liver biopsies (LB), obtained routinely 6 months after transplantation, represent a unique model...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/4/690 |
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| author | Emiliano Dalla Michela Bulfoni Daniela Cesselli Riccardo Pravisani Masaaki Hidaka Susumu Eguchi Umberto Baccarani |
| author_facet | Emiliano Dalla Michela Bulfoni Daniela Cesselli Riccardo Pravisani Masaaki Hidaka Susumu Eguchi Umberto Baccarani |
| author_sort | Emiliano Dalla |
| collection | DOAJ |
| description | Background: After liver transplantation, HCV/HIV co-infected patients present, compared to the HCV mono-infected ones, increased HCV viral load, rapid progression to liver fibrosis and higher mortality. Liver biopsies (LB), obtained routinely 6 months after transplantation, represent a unique model to assess the early events related to graft re-infection. Here, we used miRNA sequencing of LB obtained from both HCV-and HCV/HIV-infected recipients, to identify transcriptional profiles able to explain the more severe outcome of these latter. Methods: miRNAs of 3 healthy livers, 3 HCV-LB and 3 HCV/HIV-LB were sequenced by Illumina HiSeq2500 platform. The DIANA-miRPath v3.0 webserver and DIANA-microT-CDS algorithm (v5.0) were used to characterize the functions of differentially expressed (DE-) miRNAs, querying the KEGG and Gene Ontology-Biological Process databases. Results: LB obtained from infected patients were characterized, with respect to controls, by a miRNA profile related to viral infection, immune system signaling and DNA damage in HCV-induced carcinogenesis. Instead, HCV-LB and HCV/HIV-LB differed in the expression of miRNAs involved in immunological and apoptotic processes and in extracellular matrix remodeling. Conclusions: liver reinfection processes are associated with early miRNA changes. Further studies are necessary to establish their prognostic role and possible actionability. |
| first_indexed | 2024-03-09T22:20:10Z |
| format | Article |
| id | doaj.art-64e7ba83bf974b2e952e7e703ead4104 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:20:10Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-64e7ba83bf974b2e952e7e703ead41042023-11-23T19:15:29ZengMDPI AGCells2073-44092022-02-0111469010.3390/cells11040690Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression ProfileEmiliano Dalla0Michela Bulfoni1Daniela Cesselli2Riccardo Pravisani3Masaaki Hidaka4Susumu Eguchi5Umberto Baccarani6Department of Medicine, University of Udine, 33100 Udine, ItalyDepartment of Medicine, University of Udine, 33100 Udine, ItalyDepartment of Medicine, University of Udine, 33100 Udine, ItalyDepartment of Medicine, University of Udine, 33100 Udine, ItalyDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, JapanDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, JapanDepartment of Medicine, University of Udine, 33100 Udine, ItalyBackground: After liver transplantation, HCV/HIV co-infected patients present, compared to the HCV mono-infected ones, increased HCV viral load, rapid progression to liver fibrosis and higher mortality. Liver biopsies (LB), obtained routinely 6 months after transplantation, represent a unique model to assess the early events related to graft re-infection. Here, we used miRNA sequencing of LB obtained from both HCV-and HCV/HIV-infected recipients, to identify transcriptional profiles able to explain the more severe outcome of these latter. Methods: miRNAs of 3 healthy livers, 3 HCV-LB and 3 HCV/HIV-LB were sequenced by Illumina HiSeq2500 platform. The DIANA-miRPath v3.0 webserver and DIANA-microT-CDS algorithm (v5.0) were used to characterize the functions of differentially expressed (DE-) miRNAs, querying the KEGG and Gene Ontology-Biological Process databases. Results: LB obtained from infected patients were characterized, with respect to controls, by a miRNA profile related to viral infection, immune system signaling and DNA damage in HCV-induced carcinogenesis. Instead, HCV-LB and HCV/HIV-LB differed in the expression of miRNAs involved in immunological and apoptotic processes and in extracellular matrix remodeling. Conclusions: liver reinfection processes are associated with early miRNA changes. Further studies are necessary to establish their prognostic role and possible actionability.https://www.mdpi.com/2073-4409/11/4/690HCVHCV/HIVliver transplantationmiRNANGS |
| spellingShingle | Emiliano Dalla Michela Bulfoni Daniela Cesselli Riccardo Pravisani Masaaki Hidaka Susumu Eguchi Umberto Baccarani Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile Cells HCV HCV/HIV liver transplantation miRNA NGS |
| title | Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile |
| title_full | Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile |
| title_fullStr | Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile |
| title_full_unstemmed | Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile |
| title_short | Reinfection of Transplanted Livers in HCV- and HCV/HIV-Infected Patients Is Characterized by a Different MicroRNA Expression Profile |
| title_sort | reinfection of transplanted livers in hcv and hcv hiv infected patients is characterized by a different microrna expression profile |
| topic | HCV HCV/HIV liver transplantation miRNA NGS |
| url | https://www.mdpi.com/2073-4409/11/4/690 |
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