DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

Summary: Cell-free DNA (cfDNA) in spent embryo culture media (SECM) provides prospects for noninvasive preimplantation genetic testing. Here, we present a post-bisulfite-adapter-tagging (PBAT)-based whole-genome DNA methylation sequencing protocol (SECM-PBAT) for human SECM cfDNA analysis. We descri...

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Bibliographic Details
Main Authors:	Yuan Gao, Yidong Chen, Jie Qiao, Jin Huang, Lu Wen
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Series:	STAR Protocols
Subjects:	Bioinformatics Sequence Analysis Cell Biology Sequencing
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723002058

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