The secret life of tethers: the role of tethering factors in SNARE complex regulation
Trafficking in eukaryotic cells is a tightly regulated process to ensure correct cargo delivery to the proper destination organelle or plasma membrane. In this review, we focus on how the vesicle fusion machinery, the SNARE complex, is regulated by the interplay of the multisubunit tethering complex...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00042/full |
| _version_ | 1798044247206658048 |
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| author | Michelle L Dubuke Mary eMunson |
| author_facet | Michelle L Dubuke Mary eMunson |
| author_sort | Michelle L Dubuke |
| collection | DOAJ |
| description | Trafficking in eukaryotic cells is a tightly regulated process to ensure correct cargo delivery to the proper destination organelle or plasma membrane. In this review, we focus on how the vesicle fusion machinery, the SNARE complex, is regulated by the interplay of the multisubunit tethering complexes (MTC) with the SNAREs and Sec1/Munc18 (SM) proteins. Although these factors are used in different stages of membrane trafficking, e.g. Golgi to plasma membrane transport vs vacuolar fusion, and in a variety of diverse eukaryotic cell types, many commonalities between their functions are being revealed. We explore the various protein-protein interactions and findings from functional reconstitution studies in order to highlight both their common features and the differences in their modes of regulation. These studies serve as a starting point for mechanistic explorations in other systems. |
| first_indexed | 2024-04-11T23:00:45Z |
| format | Article |
| id | doaj.art-64eacf8c06a94c5db48a147dec8d19f3 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T23:00:45Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-64eacf8c06a94c5db48a147dec8d19f32022-12-22T03:58:11ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2016-05-01410.3389/fcell.2016.00042198795The secret life of tethers: the role of tethering factors in SNARE complex regulationMichelle L Dubuke0Mary eMunson1University of Massachusetts Medical SchoolUniversity of Massachusetts Medical SchoolTrafficking in eukaryotic cells is a tightly regulated process to ensure correct cargo delivery to the proper destination organelle or plasma membrane. In this review, we focus on how the vesicle fusion machinery, the SNARE complex, is regulated by the interplay of the multisubunit tethering complexes (MTC) with the SNAREs and Sec1/Munc18 (SM) proteins. Although these factors are used in different stages of membrane trafficking, e.g. Golgi to plasma membrane transport vs vacuolar fusion, and in a variety of diverse eukaryotic cell types, many commonalities between their functions are being revealed. We explore the various protein-protein interactions and findings from functional reconstitution studies in order to highlight both their common features and the differences in their modes of regulation. These studies serve as a starting point for mechanistic explorations in other systems.http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00042/fullintracellular traffickingexocystGARPHopsCOGTRAPP |
| spellingShingle | Michelle L Dubuke Mary eMunson The secret life of tethers: the role of tethering factors in SNARE complex regulation Frontiers in Cell and Developmental Biology intracellular trafficking exocyst GARP Hops COG TRAPP |
| title | The secret life of tethers: the role of tethering factors in SNARE complex regulation |
| title_full | The secret life of tethers: the role of tethering factors in SNARE complex regulation |
| title_fullStr | The secret life of tethers: the role of tethering factors in SNARE complex regulation |
| title_full_unstemmed | The secret life of tethers: the role of tethering factors in SNARE complex regulation |
| title_short | The secret life of tethers: the role of tethering factors in SNARE complex regulation |
| title_sort | secret life of tethers the role of tethering factors in snare complex regulation |
| topic | intracellular trafficking exocyst GARP Hops COG TRAPP |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2016.00042/full |
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