Diversity and Distribution Characteristics of Viruses from Soda Lakes
Viruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic ex...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/2/323 |
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| author | Ramadan A. ZeinEldin Marwa M. Ahmed Wael S. Hassanein Naglaa Elshafey Ahmed R. Sofy Hend A. Hamedo Mohamed E. Elnosary |
| author_facet | Ramadan A. ZeinEldin Marwa M. Ahmed Wael S. Hassanein Naglaa Elshafey Ahmed R. Sofy Hend A. Hamedo Mohamed E. Elnosary |
| author_sort | Ramadan A. ZeinEldin |
| collection | DOAJ |
| description | Viruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic examination of haloviruses in Wadi Al-Natrun. The discovered viromes had remarkably different taxonomic compositions. Most sequences were derived from double-stranded DNA viruses, especially from <i>Myoviridae</i>, <i>Podoviridae</i>, <i>Siphoviridae</i>, <i>Herpesviridae</i>, <i>Bicaudaviridae,</i> and <i>Phycodnaviridae</i> families; single-stranded DNA viruses, especially from the family <i>Microviridae</i>; and positive-strand RNA viruses, especially from the family <i>Potyviridae</i>. Additionally, our results showed that Myohalovirus chaoS9 has eight Contigs and is annotated to 18 proteins as follows: tail sheath protein, tco, nep, five uncharacterized proteins, HCO, major capsid protein, putative pro head protease protein, putative head assembly protein, CxxC motive protein, terl, HTH domain protein, and terS Exon 2. Additionally, Halorubrum phage CGphi46 has 19 proteins in the brine sample as follows: portal protein, 17 hypothetical proteins, major capsid protein, etc. This study reveals viral lineages, suggesting the Virus’s global dispersal more than other microorganisms. Our study clarifies how viral communities are connected and how the global environment changes. |
| first_indexed | 2024-03-11T08:46:51Z |
| format | Article |
| id | doaj.art-64eb1634626c4472a87862eb84559672 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:46:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-64eb1634626c4472a87862eb845596722023-11-16T20:41:23ZengMDPI AGGenes2073-44252023-01-0114232310.3390/genes14020323Diversity and Distribution Characteristics of Viruses from Soda LakesRamadan A. ZeinEldin0Marwa M. Ahmed1Wael S. Hassanein2Naglaa Elshafey3Ahmed R. Sofy4Hend A. Hamedo5Mohamed E. Elnosary6Deanship of Scientific Research, King AbdulAziz University, Jeddah 21589, Saudi ArabiaDepartment of Electrical and Computer Engineering, Faculty of Engineering-Girls Campus, King Abdulaziz University, Jeddah 80204, Saudi ArabiaDepartment of Industrial Engineering, Faculty of Engineering, King Abdulaziz University, Jeddah 80204, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptBotany and Microbiology Department, Faculty of Science, Arish University, Al-Arish 45511, EgyptDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptViruses are the most abundant living things and a source of genetic variation. Despite recent research, we know little about their biodiversity and geographic distribution. We used different bioinformatics tools, MG-RAST, genome detective web tools, and GenomeVx, to describe the first metagenomic examination of haloviruses in Wadi Al-Natrun. The discovered viromes had remarkably different taxonomic compositions. Most sequences were derived from double-stranded DNA viruses, especially from <i>Myoviridae</i>, <i>Podoviridae</i>, <i>Siphoviridae</i>, <i>Herpesviridae</i>, <i>Bicaudaviridae,</i> and <i>Phycodnaviridae</i> families; single-stranded DNA viruses, especially from the family <i>Microviridae</i>; and positive-strand RNA viruses, especially from the family <i>Potyviridae</i>. Additionally, our results showed that Myohalovirus chaoS9 has eight Contigs and is annotated to 18 proteins as follows: tail sheath protein, tco, nep, five uncharacterized proteins, HCO, major capsid protein, putative pro head protease protein, putative head assembly protein, CxxC motive protein, terl, HTH domain protein, and terS Exon 2. Additionally, Halorubrum phage CGphi46 has 19 proteins in the brine sample as follows: portal protein, 17 hypothetical proteins, major capsid protein, etc. This study reveals viral lineages, suggesting the Virus’s global dispersal more than other microorganisms. Our study clarifies how viral communities are connected and how the global environment changes.https://www.mdpi.com/2073-4425/14/2/323virusesviromesbrinehypersalinehalovirusesmetagenomic |
| spellingShingle | Ramadan A. ZeinEldin Marwa M. Ahmed Wael S. Hassanein Naglaa Elshafey Ahmed R. Sofy Hend A. Hamedo Mohamed E. Elnosary Diversity and Distribution Characteristics of Viruses from Soda Lakes Genes viruses viromes brine hypersaline haloviruses metagenomic |
| title | Diversity and Distribution Characteristics of Viruses from Soda Lakes |
| title_full | Diversity and Distribution Characteristics of Viruses from Soda Lakes |
| title_fullStr | Diversity and Distribution Characteristics of Viruses from Soda Lakes |
| title_full_unstemmed | Diversity and Distribution Characteristics of Viruses from Soda Lakes |
| title_short | Diversity and Distribution Characteristics of Viruses from Soda Lakes |
| title_sort | diversity and distribution characteristics of viruses from soda lakes |
| topic | viruses viromes brine hypersaline haloviruses metagenomic |
| url | https://www.mdpi.com/2073-4425/14/2/323 |
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