Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks
Transaminases are enzymes capable of stereoselective reductive amination; they are of great interest in the production of chiral building blocks. However, the use of this class of enzymes in industrial processes is often hindered by their limited stability under operational conditions. Her...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2018-05-01
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| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.chimia.ch/chimia/article/view/1441 |
| _version_ | 1818295643439890432 |
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| author | Ayoub Talbi Alami Federica Richina M. Rita Correro Yves Dudal Patrick Shahgaldian |
| author_facet | Ayoub Talbi Alami Federica Richina M. Rita Correro Yves Dudal Patrick Shahgaldian |
| author_sort | Ayoub Talbi Alami |
| collection | DOAJ |
| description |
Transaminases are enzymes capable of stereoselective reductive amination; they are of great interest in the production of chiral building blocks. However, the use of this class of enzymes in industrial processes is often hindered by their limited stability under operational conditions.
Herein, we demonstrate that a transaminase enzyme from Aspergillus terreus can be immobilized at the surface of silica nanoparticles and protected in an organosilica shell of controlled thickness. The so-protected enzyme displays a high biocatalytic activity, and additionally provides the possibility to be retained in a reactor system for continuous operation and to be recycled.
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| first_indexed | 2024-12-13T03:50:54Z |
| format | Article |
| id | doaj.art-64eb1e13cce44309b0b236feb191cc04 |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-13T03:50:54Z |
| publishDate | 2018-05-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-64eb1e13cce44309b0b236feb191cc042022-12-22T00:00:42ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242018-05-0172510.2533/chimia.2017.345Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building BlocksAyoub Talbi Alami0Federica Richina1M. Rita Correro2Yves Dudal3Patrick Shahgaldian4INOFEA AG, Hochbergerstrasse 60C, CH-4057 Basel, SwitzerlandINOFEA AG, Hochbergerstrasse 60C, CH-4057 Basel, SwitzerlandINOFEA AG, Hochbergerstrasse 60C, CH-4057 Basel, SwitzerlandINOFEA AG, Hochbergerstrasse 60C, CH-4057 Basel, SwitzerlandInstitute of Chemistry and Bioanalytics, School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, CH-4132 Muttenz, Switzerland. patrick.shahgaldian@fhnw.ch Transaminases are enzymes capable of stereoselective reductive amination; they are of great interest in the production of chiral building blocks. However, the use of this class of enzymes in industrial processes is often hindered by their limited stability under operational conditions. Herein, we demonstrate that a transaminase enzyme from Aspergillus terreus can be immobilized at the surface of silica nanoparticles and protected in an organosilica shell of controlled thickness. The so-protected enzyme displays a high biocatalytic activity, and additionally provides the possibility to be retained in a reactor system for continuous operation and to be recycled. https://www.chimia.ch/chimia/article/view/1441Chiral building blocksTransaminases |
| spellingShingle | Ayoub Talbi Alami Federica Richina M. Rita Correro Yves Dudal Patrick Shahgaldian Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks CHIMIA Chiral building blocks Transaminases |
| title | Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks |
| title_full | Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks |
| title_fullStr | Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks |
| title_full_unstemmed | Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks |
| title_short | Surface Immobilization and Shielding of a Transaminase Enzyme for the Stereoselective Synthesis of Pharmaceutically Relevant Building Blocks |
| title_sort | surface immobilization and shielding of a transaminase enzyme for the stereoselective synthesis of pharmaceutically relevant building blocks |
| topic | Chiral building blocks Transaminases |
| url | https://www.chimia.ch/chimia/article/view/1441 |
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