Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions
Several plant species of the genus Psychotria (Rubiaceae) harbor Burkholderia sp. bacteria within specialized leaf nodules. The bacteria are transmitted vertically between plant generations and have not yet been cultured outside of their host. This symbiosis is considered to be obligatory because pl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2013-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-13-0152-R |
| _version_ | 1811278051766435840 |
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| author | Aurelien L. Carlier Ulrich Omasits Christian H. Ahrens Leo Eberl |
| author_facet | Aurelien L. Carlier Ulrich Omasits Christian H. Ahrens Leo Eberl |
| author_sort | Aurelien L. Carlier |
| collection | DOAJ |
| description | Several plant species of the genus Psychotria (Rubiaceae) harbor Burkholderia sp. bacteria within specialized leaf nodules. The bacteria are transmitted vertically between plant generations and have not yet been cultured outside of their host. This symbiosis is considered to be obligatory because plants devoid of symbionts fail to develop into mature individuals. The genome of ‘Candidatus Burkholderia kirkii’ has been sequenced recently and has revealed evidence of reductive genome evolution, as shown by the proliferation of insertion sequences and the presence of numerous pseudogenes. We employed shotgun proteomics to investigate the expression of ‘Ca. B. kirkii’ proteins in the leaf nodule. Drawing from this dataset and refined comparative genomics analyses, we designed a new pseudogene prediction algorithm and improved the genome annotation. We also found conclusive evidence that nodule bacteria allocate vast resources to synthesis of secondary metabolites, possibly of the C7N aminocyclitol family. Expression of a putative 2-epi-5-valiolone synthase, a key enzyme of the C7N aminocyclitol synthesis, is high in the nodule population but downregulated in bacteria residing in the shoot apex, suggesting that production of secondary metabolites is particularly important in the leaf nodule. |
| first_indexed | 2024-04-13T00:28:48Z |
| format | Article |
| id | doaj.art-64ebc329fc984b52a1f7f4ffb02a1c17 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:28:48Z |
| publishDate | 2013-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-64ebc329fc984b52a1f7f4ffb02a1c172022-12-22T03:10:32ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062013-11-0126111325133310.1094/MPMI-05-13-0152-RProteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic PredictionsAurelien L. CarlierUlrich OmasitsChristian H. AhrensLeo EberlSeveral plant species of the genus Psychotria (Rubiaceae) harbor Burkholderia sp. bacteria within specialized leaf nodules. The bacteria are transmitted vertically between plant generations and have not yet been cultured outside of their host. This symbiosis is considered to be obligatory because plants devoid of symbionts fail to develop into mature individuals. The genome of ‘Candidatus Burkholderia kirkii’ has been sequenced recently and has revealed evidence of reductive genome evolution, as shown by the proliferation of insertion sequences and the presence of numerous pseudogenes. We employed shotgun proteomics to investigate the expression of ‘Ca. B. kirkii’ proteins in the leaf nodule. Drawing from this dataset and refined comparative genomics analyses, we designed a new pseudogene prediction algorithm and improved the genome annotation. We also found conclusive evidence that nodule bacteria allocate vast resources to synthesis of secondary metabolites, possibly of the C7N aminocyclitol family. Expression of a putative 2-epi-5-valiolone synthase, a key enzyme of the C7N aminocyclitol synthesis, is high in the nodule population but downregulated in bacteria residing in the shoot apex, suggesting that production of secondary metabolites is particularly important in the leaf nodule.https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-13-0152-R |
| spellingShingle | Aurelien L. Carlier Ulrich Omasits Christian H. Ahrens Leo Eberl Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions Molecular Plant-Microbe Interactions |
| title | Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions |
| title_full | Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions |
| title_fullStr | Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions |
| title_full_unstemmed | Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions |
| title_short | Proteomics Analysis of Psychotria Leaf Nodule Symbiosis: Improved Genome Annotation and Metabolic Predictions |
| title_sort | proteomics analysis of psychotria leaf nodule symbiosis improved genome annotation and metabolic predictions |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-05-13-0152-R |
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