Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici
Wheat take-all disease caused by Gaeumannomyces graminis var. tritici (Ggt) spreads rapidly and is highly destructive, causing severe reductions in wheat yield. Bacillus subtilis strain Z-14 that significantly controlled wheat take-all disease effectively colonized the roots of wheat seedlings. Z-14...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.923242/full |
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| author | Zhaosha Liu Zhaosha Liu Jiawen Xiao Jiawen Xiao Xuechao Zhang Xuechao Zhang Shijuan Dou Tongguo Gao Tongguo Gao Dongmei Wang Dongmei Wang Dongdong Zhang Dongdong Zhang |
| author_facet | Zhaosha Liu Zhaosha Liu Jiawen Xiao Jiawen Xiao Xuechao Zhang Xuechao Zhang Shijuan Dou Tongguo Gao Tongguo Gao Dongmei Wang Dongmei Wang Dongdong Zhang Dongdong Zhang |
| author_sort | Zhaosha Liu |
| collection | DOAJ |
| description | Wheat take-all disease caused by Gaeumannomyces graminis var. tritici (Ggt) spreads rapidly and is highly destructive, causing severe reductions in wheat yield. Bacillus subtilis strain Z-14 that significantly controlled wheat take-all disease effectively colonized the roots of wheat seedlings. Z-14 increased the metabolic activity and carbon source utilization of rhizospheric microorganisms, thus elevating average well-color development (AWCD) values and functional diversity indexes of soil microbial communities. Z-14 increased the abundance of Bacillus in the rhizosphere, which was positively correlated with AWCD and functional diversity indexes. The Z-14-treated samples acquired more linkages and relative connections between bacterial communities according to co-occurrence network analyses. After the application of Ggt, the number of linkages between fungal communities increased but later decreased, whereas Z-14 increased such interactions. Whole-genome sequencing uncovered 113 functional genes related to Z-14’s colonization ability and 10 secondary metabolite gene clusters in the strain, of which nine substances have antimicrobial activity. This study clarifies how bacterial agents like Z-14 act against phytopathogenic fungi and lays a foundation for the effective application of biocontrol agents. |
| first_indexed | 2024-04-11T12:57:12Z |
| format | Article |
| id | doaj.art-64ef14fd225246a0b48fc5298e47fb31 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T12:57:12Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-64ef14fd225246a0b48fc5298e47fb312022-12-22T04:23:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.923242923242Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. triticiZhaosha Liu0Zhaosha Liu1Jiawen Xiao2Jiawen Xiao3Xuechao Zhang4Xuechao Zhang5Shijuan Dou6Tongguo Gao7Tongguo Gao8Dongmei Wang9Dongmei Wang10Dongdong Zhang11Dongdong Zhang12College of Life Science, Hebei Agricultural University, Baoding, ChinaHebei Provincial Engineering Research Center for Resource Utilization of Agricultural Wastes, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaHebei Provincial Engineering Research Center for Resource Utilization of Agricultural Wastes, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaHebei Provincial Engineering Research Center for Resource Utilization of Agricultural Wastes, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaHebei Provincial Engineering Research Center for Resource Utilization of Agricultural Wastes, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Baoding, ChinaCollege of Life Science, Hebei Agricultural University, Baoding, ChinaHebei Provincial Engineering Research Center for Resource Utilization of Agricultural Wastes, Baoding, ChinaWheat take-all disease caused by Gaeumannomyces graminis var. tritici (Ggt) spreads rapidly and is highly destructive, causing severe reductions in wheat yield. Bacillus subtilis strain Z-14 that significantly controlled wheat take-all disease effectively colonized the roots of wheat seedlings. Z-14 increased the metabolic activity and carbon source utilization of rhizospheric microorganisms, thus elevating average well-color development (AWCD) values and functional diversity indexes of soil microbial communities. Z-14 increased the abundance of Bacillus in the rhizosphere, which was positively correlated with AWCD and functional diversity indexes. The Z-14-treated samples acquired more linkages and relative connections between bacterial communities according to co-occurrence network analyses. After the application of Ggt, the number of linkages between fungal communities increased but later decreased, whereas Z-14 increased such interactions. Whole-genome sequencing uncovered 113 functional genes related to Z-14’s colonization ability and 10 secondary metabolite gene clusters in the strain, of which nine substances have antimicrobial activity. This study clarifies how bacterial agents like Z-14 act against phytopathogenic fungi and lays a foundation for the effective application of biocontrol agents.https://www.frontiersin.org/articles/10.3389/fmicb.2022.923242/fullBacillus subtilisGaeumannomyces graminis var. triticimicrobial carbon metabolismsoil microbial diversitywhole-genome sequencing |
| spellingShingle | Zhaosha Liu Zhaosha Liu Jiawen Xiao Jiawen Xiao Xuechao Zhang Xuechao Zhang Shijuan Dou Tongguo Gao Tongguo Gao Dongmei Wang Dongmei Wang Dongdong Zhang Dongdong Zhang Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici Frontiers in Microbiology Bacillus subtilis Gaeumannomyces graminis var. tritici microbial carbon metabolism soil microbial diversity whole-genome sequencing |
| title | Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici |
| title_full | Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici |
| title_fullStr | Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici |
| title_full_unstemmed | Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici |
| title_short | Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici |
| title_sort | influence of bacillus subtilis strain z 14 on microbial communities of wheat rhizospheric soil infested with gaeumannomyces graminis var tritici |
| topic | Bacillus subtilis Gaeumannomyces graminis var. tritici microbial carbon metabolism soil microbial diversity whole-genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.923242/full |
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