Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control
Rectal swabs are routinely used by public health authorities to screen for multi-drug resistant enteric bacteria including vancomycin-resistant enterococci (VRE) and carbapenem-resistant enterobacteriaceae (CRE). Screening sensitivity can be influenced by the quality of the swabbing, whether perform...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-03-01
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| Series: | Journal of Infection and Public Health |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034117301831 |
| _version_ | 1811269077440659456 |
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| author | Sanja Glisovic Shaun Eintracht Yves Longtin Matthew Oughton Ivan Brukner |
| author_facet | Sanja Glisovic Shaun Eintracht Yves Longtin Matthew Oughton Ivan Brukner |
| author_sort | Sanja Glisovic |
| collection | DOAJ |
| description | Rectal swabs are routinely used by public health authorities to screen for multi-drug resistant enteric bacteria including vancomycin-resistant enterococci (VRE) and carbapenem-resistant enterobacteriaceae (CRE). Screening sensitivity can be influenced by the quality of the swabbing, whether performed by the patient (self-swabbing) or a healthcare practitioner. One common exclusion criterion for rectal swabs is absence of “visible soiling” from fecal matter. In our institution, this criterion excludes almost 10% of rectal swabs received in the microbiology laboratory. Furthermore, over 30% of patients in whom rectal swabs are cancelled will not be re-screened within the next 48 h, resulting in delays in removing infection prevention measures. We describe two quantitative polymerase chain reaction (qPCR)-based assays, human RNAse P and eubacterial 16S rDNA, which might serve as suitable controls for sampling adequacy. However, lower amounts of amplifiable human DNA make the 16s rDNA assay a better candidate for sample adequacy control. Keywords: PCR screening, Self-swabbing, Antibiotic resistance, Cost effective protocol, Public health screening |
| first_indexed | 2024-04-12T21:35:37Z |
| format | Article |
| id | doaj.art-64f0742a6b644b6d8ddbd5ee36595098 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-12T21:35:37Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-64f0742a6b644b6d8ddbd5ee365950982022-12-22T03:15:55ZengElsevierJournal of Infection and Public Health1876-03412018-03-01112234237Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy controlSanja Glisovic0Shaun Eintracht1Yves Longtin2Matthew Oughton3Ivan Brukner4SMBD-Jewish General Hospital, Montreal, Quebec, CanadaMedical Faculty, McGill University, Montreal, Quebec, Canada; SMBD-Jewish General Hospital, Montreal, Quebec, CanadaMedical Faculty, McGill University, Montreal, Quebec, Canada; SMBD-Jewish General Hospital, Montreal, Quebec, CanadaMedical Faculty, McGill University, Montreal, Quebec, Canada; SMBD-Jewish General Hospital, Montreal, Quebec, CanadaMedical Faculty, McGill University, Montreal, Quebec, Canada; SMBD-Jewish General Hospital, Montreal, Quebec, Canada; Corresponding author at: SMBD-Jewish General Hospital, Montreal, Quebec, Canada.Rectal swabs are routinely used by public health authorities to screen for multi-drug resistant enteric bacteria including vancomycin-resistant enterococci (VRE) and carbapenem-resistant enterobacteriaceae (CRE). Screening sensitivity can be influenced by the quality of the swabbing, whether performed by the patient (self-swabbing) or a healthcare practitioner. One common exclusion criterion for rectal swabs is absence of “visible soiling” from fecal matter. In our institution, this criterion excludes almost 10% of rectal swabs received in the microbiology laboratory. Furthermore, over 30% of patients in whom rectal swabs are cancelled will not be re-screened within the next 48 h, resulting in delays in removing infection prevention measures. We describe two quantitative polymerase chain reaction (qPCR)-based assays, human RNAse P and eubacterial 16S rDNA, which might serve as suitable controls for sampling adequacy. However, lower amounts of amplifiable human DNA make the 16s rDNA assay a better candidate for sample adequacy control. Keywords: PCR screening, Self-swabbing, Antibiotic resistance, Cost effective protocol, Public health screeninghttp://www.sciencedirect.com/science/article/pii/S1876034117301831 |
| spellingShingle | Sanja Glisovic Shaun Eintracht Yves Longtin Matthew Oughton Ivan Brukner Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control Journal of Infection and Public Health |
| title | Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control |
| title_full | Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control |
| title_fullStr | Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control |
| title_full_unstemmed | Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control |
| title_short | Rectal swab screening assays of public health importance in molecular diagnostics: Sample adequacy control |
| title_sort | rectal swab screening assays of public health importance in molecular diagnostics sample adequacy control |
| url | http://www.sciencedirect.com/science/article/pii/S1876034117301831 |
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