Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization
Spider dragline silk has unique characteristics of strength and extensibility, including supercontraction. When we use it as a biomaterial or material for textiles, it is important to suppress the effect of water on the fiber by as much as possible in order to maintain dimensional stability. In orde...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/23/8479 |
| _version_ | 1797462583673880576 |
|---|---|
| author | Tetsuo Asakura Hironori Matsuda Akira Naito Hideyasu Okamura Yu Suzuki Yunosuke Abe |
| author_facet | Tetsuo Asakura Hironori Matsuda Akira Naito Hideyasu Okamura Yu Suzuki Yunosuke Abe |
| author_sort | Tetsuo Asakura |
| collection | DOAJ |
| description | Spider dragline silk has unique characteristics of strength and extensibility, including supercontraction. When we use it as a biomaterial or material for textiles, it is important to suppress the effect of water on the fiber by as much as possible in order to maintain dimensional stability. In order to produce spider silk with a highly hydrophobic character, based on the sequence of ADF-3 silk, we produced recombinant silk (RSSP(VLI)) where all QQ sequences were replaced by VL, while single Q was replaced by I. The artificial RSSP(VLI) fiber was prepared using formic acid as the spinning solvent and methanol as the coagulant solvent. The dimensional stability and water absorption experiments of the fiber were performed for eight kinds of silk fiber. RSSP(VLI) fiber showed high dimensional stability, which is suitable for textiles. A remarkable decrease in the motion of the fiber in water was made evident by <sup>13</sup>C solid-state NMR. This study using <sup>13</sup>C solid-state NMR is the first trial to put spider silk to practical use and provide information regarding the molecular design of new recombinant spider silk materials with high dimensional stability in water, allowing recombinant spider silk proteins to be used in next-generation biomaterials and materials for textiles. |
| first_indexed | 2024-03-09T17:38:40Z |
| format | Article |
| id | doaj.art-64f1217f83694b4395d1deebc16bb18f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:38:40Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-64f1217f83694b4395d1deebc16bb18f2023-11-24T11:42:50ZengMDPI AGMolecules1420-30492022-12-012723847910.3390/molecules27238479Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR CharacterizationTetsuo Asakura0Hironori Matsuda1Akira Naito2Hideyasu Okamura3Yu Suzuki4Yunosuke Abe5Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, JapanDepartment of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, JapanDepartment of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, JapanDepartment of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, JapanDepartment of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, JapanSpiber Inc., 234-1 Mizukami, Kakuganji, Tsuruoka 997-0052, JapanSpider dragline silk has unique characteristics of strength and extensibility, including supercontraction. When we use it as a biomaterial or material for textiles, it is important to suppress the effect of water on the fiber by as much as possible in order to maintain dimensional stability. In order to produce spider silk with a highly hydrophobic character, based on the sequence of ADF-3 silk, we produced recombinant silk (RSSP(VLI)) where all QQ sequences were replaced by VL, while single Q was replaced by I. The artificial RSSP(VLI) fiber was prepared using formic acid as the spinning solvent and methanol as the coagulant solvent. The dimensional stability and water absorption experiments of the fiber were performed for eight kinds of silk fiber. RSSP(VLI) fiber showed high dimensional stability, which is suitable for textiles. A remarkable decrease in the motion of the fiber in water was made evident by <sup>13</sup>C solid-state NMR. This study using <sup>13</sup>C solid-state NMR is the first trial to put spider silk to practical use and provide information regarding the molecular design of new recombinant spider silk materials with high dimensional stability in water, allowing recombinant spider silk proteins to be used in next-generation biomaterials and materials for textiles.https://www.mdpi.com/1420-3049/27/23/8479recombinant spider silk proteindimensional stability<sup>13</sup>C NMR spectroscopy |
| spellingShingle | Tetsuo Asakura Hironori Matsuda Akira Naito Hideyasu Okamura Yu Suzuki Yunosuke Abe Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization Molecules recombinant spider silk protein dimensional stability <sup>13</sup>C NMR spectroscopy |
| title | Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization |
| title_full | Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization |
| title_fullStr | Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization |
| title_full_unstemmed | Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization |
| title_short | Recombinant Spider Silk Fiber with High Dimensional Stability in Water and Its NMR Characterization |
| title_sort | recombinant spider silk fiber with high dimensional stability in water and its nmr characterization |
| topic | recombinant spider silk protein dimensional stability <sup>13</sup>C NMR spectroscopy |
| url | https://www.mdpi.com/1420-3049/27/23/8479 |
| work_keys_str_mv | AT tetsuoasakura recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization AT hironorimatsuda recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization AT akiranaito recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization AT hideyasuokamura recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization AT yusuzuki recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization AT yunosukeabe recombinantspidersilkfiberwithhighdimensionalstabilityinwateranditsnmrcharacterization |