Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant
<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is the predominant human malaria species in Mozambique and a lead cause of mortality among children and pregnant women nationwide. Sulphadoxine/pyrimethamine (S/P) is used as first line anti...
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| Format: | Article |
| Language: | English |
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BMC
2007-03-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/6/1/35 |
| _version_ | 1828474793340436480 |
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| author | Cravo Pedro do Rosário Virgilio E Figueiredo Paula Fernandes Natércia |
| author_facet | Cravo Pedro do Rosário Virgilio E Figueiredo Paula Fernandes Natércia |
| author_sort | Cravo Pedro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is the predominant human malaria species in Mozambique and a lead cause of mortality among children and pregnant women nationwide. Sulphadoxine/pyrimethamine (S/P) is used as first line antimalarial treatment as a partner drug in combination with artesunate.</p> <p>Methods</p> <p>A total of 92 <it>P. falciparum</it>-infected blood samples, from children with uncomplicated malaria attending the Centro de Saude de Bagamoyo in the Province of Maputo-Mozambique, were screened for S/P resistance-conferring mutations in the <it>pfdhfr </it>and <it>pfdhps </it>genes using a nested mutation-specific polymerase chain reaction and restriction digestion (PCR-RFLP). The panel of genetic polymorphisms analysed included the <it>pfdhfr </it>164L mutation, previously reported to be absent or rare in Africa.</p> <p>Results</p> <p>The frequency of the S/P resistance-associated <it>pfdhfr </it>triple mutants (51I/59R/108N) and of <it>pfdhfr/pfdhps </it>quintuple mutants (51I/59R/108N <it>+ </it>437G/540E) was 93% and 47%, respectively. However, no <it>pfdhfr </it>164L mutants were detected.</p> <p>Conclusion</p> <p>The observation that a considerably high percentage of <it>P. falciparum </it>parasites contained S/P resistance-associated mutations raises concerns about the validity of this drug as first-choice treatment in Mozambique. On the other hand, no <it>pfdhfr </it>164L mutant was disclosed, corroborating the view that that this allele is still rare in Africa.</p> |
| first_indexed | 2024-12-11T06:04:24Z |
| format | Article |
| id | doaj.art-64f5047028e941269d728a88a68a0d82 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-11T06:04:24Z |
| publishDate | 2007-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-64f5047028e941269d728a88a68a0d822022-12-22T01:18:21ZengBMCMalaria Journal1475-28752007-03-01613510.1186/1475-2875-6-35Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutantCravo Pedrodo Rosário Virgilio EFigueiredo PaulaFernandes Natércia<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum </it>is the predominant human malaria species in Mozambique and a lead cause of mortality among children and pregnant women nationwide. Sulphadoxine/pyrimethamine (S/P) is used as first line antimalarial treatment as a partner drug in combination with artesunate.</p> <p>Methods</p> <p>A total of 92 <it>P. falciparum</it>-infected blood samples, from children with uncomplicated malaria attending the Centro de Saude de Bagamoyo in the Province of Maputo-Mozambique, were screened for S/P resistance-conferring mutations in the <it>pfdhfr </it>and <it>pfdhps </it>genes using a nested mutation-specific polymerase chain reaction and restriction digestion (PCR-RFLP). The panel of genetic polymorphisms analysed included the <it>pfdhfr </it>164L mutation, previously reported to be absent or rare in Africa.</p> <p>Results</p> <p>The frequency of the S/P resistance-associated <it>pfdhfr </it>triple mutants (51I/59R/108N) and of <it>pfdhfr/pfdhps </it>quintuple mutants (51I/59R/108N <it>+ </it>437G/540E) was 93% and 47%, respectively. However, no <it>pfdhfr </it>164L mutants were detected.</p> <p>Conclusion</p> <p>The observation that a considerably high percentage of <it>P. falciparum </it>parasites contained S/P resistance-associated mutations raises concerns about the validity of this drug as first-choice treatment in Mozambique. On the other hand, no <it>pfdhfr </it>164L mutant was disclosed, corroborating the view that that this allele is still rare in Africa.</p>http://www.malariajournal.com/content/6/1/35 |
| spellingShingle | Cravo Pedro do Rosário Virgilio E Figueiredo Paula Fernandes Natércia Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant Malaria Journal |
| title | Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant |
| title_full | Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant |
| title_fullStr | Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant |
| title_full_unstemmed | Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant |
| title_short | Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of <it>Plasmodium falciparum </it>from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant |
| title_sort | analysis of sulphadoxine pyrimethamine resistance conferring mutations of it plasmodium falciparum it from mozambique reveals the absence of the dihydrofolate reductase 164l mutant |
| url | http://www.malariajournal.com/content/6/1/35 |
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