Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities
Background: This study is designed to discover a method for delivering an efficient potent pheophytin a (pheo-a) into more absorbed and small polymeric ethyl cellulose (EC) microparticles. Methods: Silica gel and Sephadex LH-20 columns were used to isolate pheo-a from the chloroform extract of the e...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1501 |
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| author | Hamdoon A. Mohammed Mohsen S. Al-Omar Mahmoud Zaki El-Readi Ahmad H. Alhowail Maha A. Aldubayan Ahmed A. H. Abdellatif |
| author_facet | Hamdoon A. Mohammed Mohsen S. Al-Omar Mahmoud Zaki El-Readi Ahmad H. Alhowail Maha A. Aldubayan Ahmed A. H. Abdellatif |
| author_sort | Hamdoon A. Mohammed |
| collection | DOAJ |
| description | Background: This study is designed to discover a method for delivering an efficient potent pheophytin a (pheo-a) into more absorbed and small polymeric ethyl cellulose (EC) microparticles. Methods: Silica gel and Sephadex LH-20 columns were used to isolate pheo-a from the chloroform extract of the edible plant, <i>Suaeda vermiculata</i>. Pheo-a was incorporated into EC microparticles using emulsion-solvent techniques. The antioxidant activity of pheo-a microparticles was confirmed by the level of superoxide radical (SOD), nitric oxide (NO), and reducing power (RP) methods. Meanwhile, the cytotoxic effect of the product was investigated on MCF-7 cells using MTT assay. Results: Pheo-a was isolated from <i>S. vermiculata</i> in a 12% concentration of the total chloroform extract. The structures were confirmed by NMR and IR spectroscopic analysis. The formulated microparticles were uniform, completely dispersed in the aqueous media, compatible as ingredients, and had a mean diameter of 139 ± 1.56 µm as measured by a particle size analyzer. Pheo-a demonstrated a valuable antioxidant activity when compared with ascorbic acid. The IC<sub>50</sub> values of pheo-a microparticles were 200.5 and 137.7 µg/mL for SOD, and NO respectively. The reducing power of pheo-a microparticles was more potent than ascorbic acid and had a 4.2 µg/mL for IC<sub>50</sub> value. Pheo-a microparticles did not show notable cytotoxicity on the MCF-7 cell line (IC<sub>50</sub> = 35.9 µg/mL) compared with doxorubicin (IC<sub>50</sub> = 3.2 µg/mL). Conclusions: the results showed that water-soluble pheo-a microparticles were prepared with a valuable antioxidant activity in a wide range of concentrations with a noteworthy cytotoxic effect. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T08:43:28Z |
| publishDate | 2019-04-01 |
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| series | Molecules |
| spelling | doaj.art-64f66a7d65a346339577c47483a5432e2022-12-22T00:30:41ZengMDPI AGMolecules1420-30492019-04-01248150110.3390/molecules24081501molecules24081501Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic ActivitiesHamdoon A. Mohammed0Mohsen S. Al-Omar1Mahmoud Zaki El-Readi2Ahmad H. Alhowail3Maha A. Aldubayan4Ahmed A. H. Abdellatif5Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11371, EgyptDepartment of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, 51452 Al Qassim, Kingdom of Saudi ArabiaDepartment of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, 51452 Al Qassim, Kingdom of Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, 51452 Al Qassim, Kingdom of Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Qassim University, 51452 Al Qassim, Kingdom of Saudi ArabiaBackground: This study is designed to discover a method for delivering an efficient potent pheophytin a (pheo-a) into more absorbed and small polymeric ethyl cellulose (EC) microparticles. Methods: Silica gel and Sephadex LH-20 columns were used to isolate pheo-a from the chloroform extract of the edible plant, <i>Suaeda vermiculata</i>. Pheo-a was incorporated into EC microparticles using emulsion-solvent techniques. The antioxidant activity of pheo-a microparticles was confirmed by the level of superoxide radical (SOD), nitric oxide (NO), and reducing power (RP) methods. Meanwhile, the cytotoxic effect of the product was investigated on MCF-7 cells using MTT assay. Results: Pheo-a was isolated from <i>S. vermiculata</i> in a 12% concentration of the total chloroform extract. The structures were confirmed by NMR and IR spectroscopic analysis. The formulated microparticles were uniform, completely dispersed in the aqueous media, compatible as ingredients, and had a mean diameter of 139 ± 1.56 µm as measured by a particle size analyzer. Pheo-a demonstrated a valuable antioxidant activity when compared with ascorbic acid. The IC<sub>50</sub> values of pheo-a microparticles were 200.5 and 137.7 µg/mL for SOD, and NO respectively. The reducing power of pheo-a microparticles was more potent than ascorbic acid and had a 4.2 µg/mL for IC<sub>50</sub> value. Pheo-a microparticles did not show notable cytotoxicity on the MCF-7 cell line (IC<sub>50</sub> = 35.9 µg/mL) compared with doxorubicin (IC<sub>50</sub> = 3.2 µg/mL). Conclusions: the results showed that water-soluble pheo-a microparticles were prepared with a valuable antioxidant activity in a wide range of concentrations with a noteworthy cytotoxic effect.https://www.mdpi.com/1420-3049/24/8/1501<i>Suaeda vermiculata</i>pheophytin apolymeric microparticlesethyl celluloseantioxidant activity |
| spellingShingle | Hamdoon A. Mohammed Mohsen S. Al-Omar Mahmoud Zaki El-Readi Ahmad H. Alhowail Maha A. Aldubayan Ahmed A. H. Abdellatif Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities Molecules <i>Suaeda vermiculata</i> pheophytin a polymeric microparticles ethyl cellulose antioxidant activity |
| title | Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities |
| title_full | Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities |
| title_fullStr | Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities |
| title_full_unstemmed | Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities |
| title_short | Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from <i>Suaeda vermiculata</i> for Antioxidant and Cytotoxic Activities |
| title_sort | formulation of ethyl cellulose microparticles incorporated pheophytin a isolated from i suaeda vermiculata i for antioxidant and cytotoxic activities |
| topic | <i>Suaeda vermiculata</i> pheophytin a polymeric microparticles ethyl cellulose antioxidant activity |
| url | https://www.mdpi.com/1420-3049/24/8/1501 |
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