Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract
We report on employing in vitro biosynthesized ZnO nanoparticles using <i>L. pubescens</i> shoot methanol extract (50 and 100 mg LP–ZnO NPs) to examine their antimicrobial efficacy against <i>Pseudomonas aeruginosa</i> (ATCC27853), <i>Staphylococcus aureus</i> (AT...
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MDPI AG
2022-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/22/11613 |
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| author | Abu ElGasim A. Yagoub Ghedeir M. Al-Shammari Laila Naif Al-Harbi Pandurangan Subash-Babu Rasha Elsayim Mohammed A. Mohammed Mohammed Abdo Yahya Sndos Z. A. Fattiny |
| author_facet | Abu ElGasim A. Yagoub Ghedeir M. Al-Shammari Laila Naif Al-Harbi Pandurangan Subash-Babu Rasha Elsayim Mohammed A. Mohammed Mohammed Abdo Yahya Sndos Z. A. Fattiny |
| author_sort | Abu ElGasim A. Yagoub |
| collection | DOAJ |
| description | We report on employing in vitro biosynthesized ZnO nanoparticles using <i>L. pubescens</i> shoot methanol extract (50 and 100 mg LP–ZnO NPs) to examine their antimicrobial efficacy against <i>Pseudomonas aeruginosa</i> (ATCC27853), <i>Staphylococcus aureus</i> (ATCC 29213), <i>Aspergillus niger</i> (ATCC 16404 NA), and <i>Aspergillus terreus</i> (TCC 10029). The formation and stability of the investigated ZnO nanoparticles were proven by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), UV–vis spectroscopy, X-ray diffraction (XRD), and thermal gravimetric analysis (TGA). The ZnO nanoparticles were rod-shaped (width: 10.76–30.93 nm). The nanoparticles in dimethyl sulfoxide (DMSO) outperformed their water counterparts in terms of their zones of inhibition (ZIs) (marginal means of 12.5 and 8.19 mm, respectively) and minimum inhibition concentrations (MICs) (means of 4.40 and 8.54 mg/mL, respectively). The ZI means for <i>S. aureus</i>, <i>P. aeruginosa</i>, <i>A. terreus</i>, and <i>A. niger</i> were 10.50, 6.13, 12.5, and 11.5 mm, respectively. When treating <i>S. aureus</i> and <i>P. aeruginosa</i>, the ZI of the 50 mg LP–ZnO NPs in water was better (14 mm), with a lower MIC and lower minimum bactericidal/fungicide concentrations (MBC/MFC) (7.22 and 4.88 mg/mL, respectively) than the ZnO and control drugs. The SEM images showed cellular alterations in the surface shapes after the LP–ZnO-NP treatments. Biosynthesized LP–ZnO NPs could have beneficial antibacterial properties, which could allow for future contributions to the development of new antimicrobial drugs. |
| first_indexed | 2024-03-09T18:29:19Z |
| format | Article |
| id | doaj.art-65042cb427a2485696198aa09dfdddc9 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T18:29:19Z |
| publishDate | 2022-11-01 |
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| series | Applied Sciences |
| spelling | doaj.art-65042cb427a2485696198aa09dfdddc92023-11-24T07:38:07ZengMDPI AGApplied Sciences2076-34172022-11-0112221161310.3390/app122211613Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol ExtractAbu ElGasim A. Yagoub0Ghedeir M. Al-Shammari1Laila Naif Al-Harbi2Pandurangan Subash-Babu3Rasha Elsayim4Mohammed A. Mohammed5Mohammed Abdo Yahya6Sndos Z. A. Fattiny7Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Microbiology, College of Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaWe report on employing in vitro biosynthesized ZnO nanoparticles using <i>L. pubescens</i> shoot methanol extract (50 and 100 mg LP–ZnO NPs) to examine their antimicrobial efficacy against <i>Pseudomonas aeruginosa</i> (ATCC27853), <i>Staphylococcus aureus</i> (ATCC 29213), <i>Aspergillus niger</i> (ATCC 16404 NA), and <i>Aspergillus terreus</i> (TCC 10029). The formation and stability of the investigated ZnO nanoparticles were proven by transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), UV–vis spectroscopy, X-ray diffraction (XRD), and thermal gravimetric analysis (TGA). The ZnO nanoparticles were rod-shaped (width: 10.76–30.93 nm). The nanoparticles in dimethyl sulfoxide (DMSO) outperformed their water counterparts in terms of their zones of inhibition (ZIs) (marginal means of 12.5 and 8.19 mm, respectively) and minimum inhibition concentrations (MICs) (means of 4.40 and 8.54 mg/mL, respectively). The ZI means for <i>S. aureus</i>, <i>P. aeruginosa</i>, <i>A. terreus</i>, and <i>A. niger</i> were 10.50, 6.13, 12.5, and 11.5 mm, respectively. When treating <i>S. aureus</i> and <i>P. aeruginosa</i>, the ZI of the 50 mg LP–ZnO NPs in water was better (14 mm), with a lower MIC and lower minimum bactericidal/fungicide concentrations (MBC/MFC) (7.22 and 4.88 mg/mL, respectively) than the ZnO and control drugs. The SEM images showed cellular alterations in the surface shapes after the LP–ZnO-NP treatments. Biosynthesized LP–ZnO NPs could have beneficial antibacterial properties, which could allow for future contributions to the development of new antimicrobial drugs.https://www.mdpi.com/2076-3417/12/22/11613lavender shootsbioactive compoundsbiosynthesisZnO nanorodzone of inhibition |
| spellingShingle | Abu ElGasim A. Yagoub Ghedeir M. Al-Shammari Laila Naif Al-Harbi Pandurangan Subash-Babu Rasha Elsayim Mohammed A. Mohammed Mohammed Abdo Yahya Sndos Z. A. Fattiny Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract Applied Sciences lavender shoots bioactive compounds biosynthesis ZnO nanorod zone of inhibition |
| title | Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract |
| title_full | Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract |
| title_fullStr | Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract |
| title_full_unstemmed | Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract |
| title_short | Antimicrobial Properties of Zinc Oxide Nanoparticles Synthesized from <i>Lavandula pubescens</i> Shoot Methanol Extract |
| title_sort | antimicrobial properties of zinc oxide nanoparticles synthesized from i lavandula pubescens i shoot methanol extract |
| topic | lavender shoots bioactive compounds biosynthesis ZnO nanorod zone of inhibition |
| url | https://www.mdpi.com/2076-3417/12/22/11613 |
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