Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i>
During our search for ethanol-producing basidiomycete fungi for a wide range of substrates, we isolated <i>Phlebia acerina</i>, which is a white rot basidiomycete fungus. It favorably converted starch into ethanol with approximately 70% yield. Although the yield decreased as the starch c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/7/3/116 |
| _version_ | 1797519347424428032 |
|---|---|
| author | Kenji Okamoto Takato Goda Takeru Yamada Masafumi Nagoshi |
| author_facet | Kenji Okamoto Takato Goda Takeru Yamada Masafumi Nagoshi |
| author_sort | Kenji Okamoto |
| collection | DOAJ |
| description | During our search for ethanol-producing basidiomycete fungi for a wide range of substrates, we isolated <i>Phlebia acerina</i>, which is a white rot basidiomycete fungus. It favorably converted starch into ethanol with approximately 70% yield. Although the yield decreased as the starch concentration increased, growth and fermentation were observed even at 200 g/L of starch. <i>P. acerina</i> produced ethanol from glucose, galactose, mannose, xylose, cellobiose, and maltose with 93%, 91%, 86%, 72%, 92%, and 68% yields, respectively. Additionally, <i>P. acerina</i>, which secreted xylanase and xylosidase, was capable of assimilating xylan and directly converting it to ethanol with a yield of 63%. Furthermore, <i>P. acerina</i> produced ethanol directly from acorns, which are plant fruits containing starch and tannins, with a yield of 70%. Tannin delayed mycelia growth, thus prolonging ethanol production; however, this did not particularly affect the yield. These results were similar to those of fermentation in a medium with the same amounts of starch and tannin as the target crop acorn, thus suggesting that <i>P. acerina</i> could successfully produce environmentally friendly ethanol from starch-containing lignocellulosic biomass, unlike previously reported ethanol-producing basidiomycete fungi. |
| first_indexed | 2024-03-10T07:41:35Z |
| format | Article |
| id | doaj.art-65043accdd024c9c9da81f1eb61826e8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T07:41:35Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-65043accdd024c9c9da81f1eb61826e82023-11-22T12:59:22ZengMDPI AGFermentation2311-56372021-07-017311610.3390/fermentation7030116Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i>Kenji Okamoto0Takato Goda1Takeru Yamada2Masafumi Nagoshi3Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, JapanDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, JapanDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, JapanDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, JapanDuring our search for ethanol-producing basidiomycete fungi for a wide range of substrates, we isolated <i>Phlebia acerina</i>, which is a white rot basidiomycete fungus. It favorably converted starch into ethanol with approximately 70% yield. Although the yield decreased as the starch concentration increased, growth and fermentation were observed even at 200 g/L of starch. <i>P. acerina</i> produced ethanol from glucose, galactose, mannose, xylose, cellobiose, and maltose with 93%, 91%, 86%, 72%, 92%, and 68% yields, respectively. Additionally, <i>P. acerina</i>, which secreted xylanase and xylosidase, was capable of assimilating xylan and directly converting it to ethanol with a yield of 63%. Furthermore, <i>P. acerina</i> produced ethanol directly from acorns, which are plant fruits containing starch and tannins, with a yield of 70%. Tannin delayed mycelia growth, thus prolonging ethanol production; however, this did not particularly affect the yield. These results were similar to those of fermentation in a medium with the same amounts of starch and tannin as the target crop acorn, thus suggesting that <i>P. acerina</i> could successfully produce environmentally friendly ethanol from starch-containing lignocellulosic biomass, unlike previously reported ethanol-producing basidiomycete fungi.https://www.mdpi.com/2311-5637/7/3/116basidiomycete<i>Phlebia acerina</i>ethanolfermentationxylanacorn |
| spellingShingle | Kenji Okamoto Takato Goda Takeru Yamada Masafumi Nagoshi Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> Fermentation basidiomycete <i>Phlebia acerina</i> ethanol fermentation xylan acorn |
| title | Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> |
| title_full | Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> |
| title_fullStr | Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> |
| title_full_unstemmed | Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> |
| title_short | Direct Ethanol Production from Xylan and Acorn Using the Starch-Fermenting Basidiomycete Fungus <i>Phlebia acerina</i> |
| title_sort | direct ethanol production from xylan and acorn using the starch fermenting basidiomycete fungus i phlebia acerina i |
| topic | basidiomycete <i>Phlebia acerina</i> ethanol fermentation xylan acorn |
| url | https://www.mdpi.com/2311-5637/7/3/116 |
| work_keys_str_mv | AT kenjiokamoto directethanolproductionfromxylanandacornusingthestarchfermentingbasidiomycetefungusiphlebiaacerinai AT takatogoda directethanolproductionfromxylanandacornusingthestarchfermentingbasidiomycetefungusiphlebiaacerinai AT takeruyamada directethanolproductionfromxylanandacornusingthestarchfermentingbasidiomycetefungusiphlebiaacerinai AT masafuminagoshi directethanolproductionfromxylanandacornusingthestarchfermentingbasidiomycetefungusiphlebiaacerinai |