Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin
In the present study, a method for the separation by macroporous resins of 10-hydroxycamptothecin (HCPT) from the extracts remaining from the production of camptothecin (CPT) has been developed. The adsorption/desorption characteristics of HCPT on eight macroporous resins were compared. Of these, th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2009-03-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/026361709789625298 |
| _version_ | 1797285530175537152 |
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| author | Lei Yang Chunjian Zhao Chunying Li Jiahui Li Xiaojuan Li Yuangang Zu |
| author_facet | Lei Yang Chunjian Zhao Chunying Li Jiahui Li Xiaojuan Li Yuangang Zu |
| author_sort | Lei Yang |
| collection | DOAJ |
| description | In the present study, a method for the separation by macroporous resins of 10-hydroxycamptothecin (HCPT) from the extracts remaining from the production of camptothecin (CPT) has been developed. The adsorption/desorption characteristics of HCPT on eight macroporous resins were compared. Of these, the X-5 resin offered the best adsorption/desorption densities for HCPT, with its adsorption data being best fitted by the Langmuir and Freundlich isotherms. Dynamic adsorption/desorption experiments were carried out in a column packed with X-5 resin in order to optimize the separation process. The optimum parameters for adsorption were as follows: sample solution concentration = 0.3156 mg/mℓ, processing volume = 5 BV (bed volume), flow rate = 4 BV/h, temperature = 313 K, pH = 5; for desorption, the corresponding parameters were: 10–60% ethanol as eluent, processing volume = 2 BV, flow rate = 4 BV/h. After being treated with X-5 resin, the content of HCPT in the dry product increased from 0.30% to 7.19%, i.e. a 23.9-fold increase when compared to the untreated extract, with the recovery yield of HCPT being 82.1%. |
| first_indexed | 2024-03-07T18:03:35Z |
| format | Article |
| id | doaj.art-6509979ca59c45dda4d3ccd10d1a28b2 |
| institution | Directory Open Access Journal |
| issn | 0263-6174 2048-4038 |
| language | English |
| last_indexed | 2024-03-07T18:03:35Z |
| publishDate | 2009-03-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj.art-6509979ca59c45dda4d3ccd10d1a28b22024-03-02T09:53:38ZengSAGE PublicationsAdsorption Science & Technology0263-61742048-40382009-03-012710.1260/026361709789625298Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of CamptothecinLei YangChunjian ZhaoChunying LiJiahui LiXiaojuan LiYuangang ZuIn the present study, a method for the separation by macroporous resins of 10-hydroxycamptothecin (HCPT) from the extracts remaining from the production of camptothecin (CPT) has been developed. The adsorption/desorption characteristics of HCPT on eight macroporous resins were compared. Of these, the X-5 resin offered the best adsorption/desorption densities for HCPT, with its adsorption data being best fitted by the Langmuir and Freundlich isotherms. Dynamic adsorption/desorption experiments were carried out in a column packed with X-5 resin in order to optimize the separation process. The optimum parameters for adsorption were as follows: sample solution concentration = 0.3156 mg/mℓ, processing volume = 5 BV (bed volume), flow rate = 4 BV/h, temperature = 313 K, pH = 5; for desorption, the corresponding parameters were: 10–60% ethanol as eluent, processing volume = 2 BV, flow rate = 4 BV/h. After being treated with X-5 resin, the content of HCPT in the dry product increased from 0.30% to 7.19%, i.e. a 23.9-fold increase when compared to the untreated extract, with the recovery yield of HCPT being 82.1%.https://doi.org/10.1260/026361709789625298 |
| spellingShingle | Lei Yang Chunjian Zhao Chunying Li Jiahui Li Xiaojuan Li Yuangang Zu Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin Adsorption Science & Technology |
| title | Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin |
| title_full | Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin |
| title_fullStr | Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin |
| title_full_unstemmed | Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin |
| title_short | Separation by Macroporous Resins of 10-Hydroxycamptothecin from the Remainder Extracts in the Production of Camptothecin |
| title_sort | separation by macroporous resins of 10 hydroxycamptothecin from the remainder extracts in the production of camptothecin |
| url | https://doi.org/10.1260/026361709789625298 |
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