Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures
Establishing optimal growth conditions for secondary metabolites production in vitro is vital for the biotechnological development of medicinal plants. In the present work we investigate the relations between the supplementation of plant growth regulators (benzyl adenine and indole-3-butyric acid) t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bulgarian Academy of Sciences
2018-03-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2018/vol_22.1/files/22.1_08.pdf |
| _version_ | 1818468104149139456 |
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| author | Nia Petrova Petya Koleva Violeta Velikova Tsonko Tsonev Tonya Andreeva Stefka Taneva Sashka Krumova Kalina Danova |
| author_facet | Nia Petrova Petya Koleva Violeta Velikova Tsonko Tsonev Tonya Andreeva Stefka Taneva Sashka Krumova Kalina Danova |
| author_sort | Nia Petrova |
| collection | DOAJ |
| description | Establishing optimal growth conditions for secondary metabolites production in vitro is vital for the biotechnological development of medicinal plants. In the present work we investigate the relations between the supplementation of plant growth regulators (benzyl adenine and indole-3-butyric acid) to in vitro shoot cultures of the medicinal plant Artemisia alba Turra, the productivity of antioxidant polyphenolic compounds and the structural and functional characteristics of the photosynthetic apparatus. We assayed the structural characteristics of isolated thylakoid membranes from the aerial parts by means of circular dichroism spectroscopy and atomic force microscopy, and the photosynthetic performance by pulse amplitude fluorescence modulated imaging. Although a complex relationship between benzyl adenine and indole-3-butyric acid supplementation, the polyphenolic levels and the architecture and functionality of the photosynthetic thylakoid membranes was revealed, a clear correlation was established between the concentration of the produced polyphenolic compounds and the quantum yield of photosystem II. Our data demonstrate that there is an optimal combination of the applied plant growth regulators that triggers efficient photosynthesis and high phenolics production. |
| first_indexed | 2024-04-13T21:09:23Z |
| format | Article |
| id | doaj.art-650a84fc392f495088e43e229d2cc853 |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-04-13T21:09:23Z |
| publishDate | 2018-03-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-650a84fc392f495088e43e229d2cc8532022-12-22T02:29:52ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212018-03-01221738210.7546/ijba.2018.22.1.73-82Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue culturesNia Petrova0Petya KolevaVioleta VelikovaTsonko TsonevTonya AndreevaStefka TanevaSashka KrumovaKalina DanovaInstitute of Biophysics and Biomedical Engineering, Bulgarian Academy of SciencesEstablishing optimal growth conditions for secondary metabolites production in vitro is vital for the biotechnological development of medicinal plants. In the present work we investigate the relations between the supplementation of plant growth regulators (benzyl adenine and indole-3-butyric acid) to in vitro shoot cultures of the medicinal plant Artemisia alba Turra, the productivity of antioxidant polyphenolic compounds and the structural and functional characteristics of the photosynthetic apparatus. We assayed the structural characteristics of isolated thylakoid membranes from the aerial parts by means of circular dichroism spectroscopy and atomic force microscopy, and the photosynthetic performance by pulse amplitude fluorescence modulated imaging. Although a complex relationship between benzyl adenine and indole-3-butyric acid supplementation, the polyphenolic levels and the architecture and functionality of the photosynthetic thylakoid membranes was revealed, a clear correlation was established between the concentration of the produced polyphenolic compounds and the quantum yield of photosystem II. Our data demonstrate that there is an optimal combination of the applied plant growth regulators that triggers efficient photosynthesis and high phenolics production.http://www.biomed.bas.bg/bioautomation/2018/vol_22.1/files/22.1_08.pdfPhotosynthesysArtemisia alba TurraPolyphenolic compoundsin vitro shoot cultures |
| spellingShingle | Nia Petrova Petya Koleva Violeta Velikova Tsonko Tsonev Tonya Andreeva Stefka Taneva Sashka Krumova Kalina Danova Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures International Journal Bioautomation Photosynthesys Artemisia alba Turra Polyphenolic compounds in vitro shoot cultures |
| title | Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures |
| title_full | Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures |
| title_fullStr | Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures |
| title_full_unstemmed | Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures |
| title_short | Relations between photosynthetic performance and polyphenolics productivity of Artemisia alba Turra in in vitro tissue cultures |
| title_sort | relations between photosynthetic performance and polyphenolics productivity of artemisia alba turra in in vitro tissue cultures |
| topic | Photosynthesys Artemisia alba Turra Polyphenolic compounds in vitro shoot cultures |
| url | http://www.biomed.bas.bg/bioautomation/2018/vol_22.1/files/22.1_08.pdf |
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