Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis
The aim of the work was to study the growth dependence on amino acids in Yersinia pestis strains of different phylogenetic branches of the antiqua biovar and to determine the genetic basis of auxotrophy of these strains.Materials and methods. We used 38 strains of Y. pestis, the main subspecies of t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2024-01-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1903 |
| _version_ | 1797220735422300160 |
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| author | M. A. Makashova L. M. Kukleva N. S. Chervyakova E. A. Naryshkina A. V. Kovrizhnikov G. A. Eroshenko I. G. Shvidenko V. V. Kutyrev |
| author_facet | M. A. Makashova L. M. Kukleva N. S. Chervyakova E. A. Naryshkina A. V. Kovrizhnikov G. A. Eroshenko I. G. Shvidenko V. V. Kutyrev |
| author_sort | M. A. Makashova |
| collection | DOAJ |
| description | The aim of the work was to study the growth dependence on amino acids in Yersinia pestis strains of different phylogenetic branches of the antiqua biovar and to determine the genetic basis of auxotrophy of these strains.Materials and methods. We used 38 strains of Y. pestis, the main subspecies of the antiqua biovar, isolated in various foci of the world in the period of 1928–2020. The nutritional requirements of the strains were determined by incubation on Difco minimal agar with different sets of amino acids. Phylogenetic analysis of the strains was carried out using the Wombac 2.0 and SeaView 5.0.5 programs. Comparative analysis of the nucleotide sequences of the genes was performed using the BLAST algorithm and the Mega 7.0 program.Results and discussion. The phylogenetic appurtenance of the investigated Y. pestis strains of antiqua biovar to the phylogenetic branches 0.ANT3, 0.ANT5, 1.ANT, 2.ANT3, 3.ANT2, 4.ANT has been determined. It is established that all studied strains have a common dependence of growth on three amino acids – phenylalanine, threonine, and methionine. In the majority of the strains of antiqua biovar of all phylogenetic branches, growth is also dependent on the presence of cysteine in the medium, except for a part of the strains of the phylogenetic branch 4.ANT. In 14 genes of sulfur and cysteine metabolism, 19 mutations have been identified. Each phylogenetic group of the antique biovar has a distinct profile of mutations in genes involved in cysteine biosynthesis. The leucine requirement of the strains belonging to phylogenetic branch 0.ANT5 has been established, possibly caused by a frame shift in the leuA gene. Strains of the 1.ANT branch isolated in the Democratic Republic of the Congo demonstrate an additional proline-dependent growth. The defined nutritional requirements in the strains and the genetic causes of auxotrophy complement the phenotypic and genetic characteristics of the phylogenetic branches of the antiqua biovar and can be used as genetic markers for the differentiation of these strains. |
| first_indexed | 2024-03-08T13:40:15Z |
| format | Article |
| id | doaj.art-650c0702640d4c429ae1394af5a37aa8 |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:54:16Z |
| publishDate | 2024-01-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-650c0702640d4c429ae1394af5a37aa82024-04-05T16:47:18ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2024-01-01049610510.21055/0370-1069-2023-4-96-1051513Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic BasisM. A. Makashova0L. M. Kukleva1N. S. Chervyakova2E. A. Naryshkina3A. V. Kovrizhnikov4G. A. Eroshenko5I. G. Shvidenko6V. V. Kutyrev7Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”Russian Research Anti-Plague Institute “Microbe”The aim of the work was to study the growth dependence on amino acids in Yersinia pestis strains of different phylogenetic branches of the antiqua biovar and to determine the genetic basis of auxotrophy of these strains.Materials and methods. We used 38 strains of Y. pestis, the main subspecies of the antiqua biovar, isolated in various foci of the world in the period of 1928–2020. The nutritional requirements of the strains were determined by incubation on Difco minimal agar with different sets of amino acids. Phylogenetic analysis of the strains was carried out using the Wombac 2.0 and SeaView 5.0.5 programs. Comparative analysis of the nucleotide sequences of the genes was performed using the BLAST algorithm and the Mega 7.0 program.Results and discussion. The phylogenetic appurtenance of the investigated Y. pestis strains of antiqua biovar to the phylogenetic branches 0.ANT3, 0.ANT5, 1.ANT, 2.ANT3, 3.ANT2, 4.ANT has been determined. It is established that all studied strains have a common dependence of growth on three amino acids – phenylalanine, threonine, and methionine. In the majority of the strains of antiqua biovar of all phylogenetic branches, growth is also dependent on the presence of cysteine in the medium, except for a part of the strains of the phylogenetic branch 4.ANT. In 14 genes of sulfur and cysteine metabolism, 19 mutations have been identified. Each phylogenetic group of the antique biovar has a distinct profile of mutations in genes involved in cysteine biosynthesis. The leucine requirement of the strains belonging to phylogenetic branch 0.ANT5 has been established, possibly caused by a frame shift in the leuA gene. Strains of the 1.ANT branch isolated in the Democratic Republic of the Congo demonstrate an additional proline-dependent growth. The defined nutritional requirements in the strains and the genetic causes of auxotrophy complement the phenotypic and genetic characteristics of the phylogenetic branches of the antiqua biovar and can be used as genetic markers for the differentiation of these strains.https://journal.microbe.ru/jour/article/view/1903plague agentantiqua biovarand nutritional requirements of strains |
| spellingShingle | M. A. Makashova L. M. Kukleva N. S. Chervyakova E. A. Naryshkina A. V. Kovrizhnikov G. A. Eroshenko I. G. Shvidenko V. V. Kutyrev Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis Проблемы особо опасных инфекций plague agent antiqua biovar and nutritional requirements of strains |
| title | Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis |
| title_full | Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis |
| title_fullStr | Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis |
| title_full_unstemmed | Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis |
| title_short | Auxotrophy of <I>Yersinia pestis</I> Strains of Antiqua Biovar and Its Genetic Basis |
| title_sort | auxotrophy of i yersinia pestis i strains of antiqua biovar and its genetic basis |
| topic | plague agent antiqua biovar and nutritional requirements of strains |
| url | https://journal.microbe.ru/jour/article/view/1903 |
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