Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle
Fermented distillers’ grains (FDG) are commonly used to enhance the health and metabolic processes of livestock and poultry by regulating the composition and activity of the intestinal microbiota. Nevertheless, there is a scarcity of research on the effects of the FDG diet on the gastrointestinal mi...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/22/3437 |
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| author | Guangxia He Chao Chen Shihui Mei Ze Chen Rong Zhang Tiantian Zhang Duhan Xu Mingming Zhu Xiaofen Luo Chengrong Zeng Bijun Zhou Kaigong Wang Erpeng Zhu Zhentao Cheng |
| author_facet | Guangxia He Chao Chen Shihui Mei Ze Chen Rong Zhang Tiantian Zhang Duhan Xu Mingming Zhu Xiaofen Luo Chengrong Zeng Bijun Zhou Kaigong Wang Erpeng Zhu Zhentao Cheng |
| author_sort | Guangxia He |
| collection | DOAJ |
| description | Fermented distillers’ grains (FDG) are commonly used to enhance the health and metabolic processes of livestock and poultry by regulating the composition and activity of the intestinal microbiota. Nevertheless, there is a scarcity of research on the effects of the FDG diet on the gastrointestinal microbiota and its metabolites in cattle. This study examines the impact of FDG dietary supplements on the gastrointestinal flora and metabolic profile of Guanling cattle. Eighteen cattle were randomly assigned to three treatment groups with six replicates per group. The treatments included a basal diet (BD), a 15% concentrate replaced by FDG (15% FDG) in the basal diet, and a 30% concentrate replaced by FDG (30% FDG) in the basal diet. Each group was fed for a duration of 60 days. At the conclusion of the experimental period, three cattle were randomly chosen from each group for slaughter and the microbial community structure and metabolic mapping of their abomasal and cecal contents were analyzed, utilizing 16S rDNA sequencing and LC-MS technology, respectively. At the phylum level, there was a significant increase in Bacteroidetes in both the abomasum and cecum for the 30%FDG group (<i>p</i> < 0.05). Additionally, there was a significant reduction in potential pathogenic bacteria such as Spirochetes and Proteobacteria for both the 15%FDG and 30%FDG groups (<i>p</i> < 0.05). At the genus level, there was a significant increase (<i>p</i> < 0.05) in Ruminococcaceae_UCG-010, Prevotellaceae_UCG-001, and Ruminococcaceae_UCG-005 fiber degradation bacteria. Non-target metabolomics analysis indicated that the FDG diet significantly impacted primary bile acid biosynthesis, bile secretion, choline metabolism in cancer, and other metabolic pathways (<i>p</i> < 0.05). There is a noteworthy correlation between the diverse bacterial genera and metabolites found in the abomasal and cecal contents of Guanling cattle, as demonstrated by correlation analysis. In conclusion, our findings suggest that partially substituting FDG for conventional feed leads to beneficial effects on both the structure of the gastrointestinal microbial community and the metabolism of its contents in Guanling cattle. These findings offer a scientific point of reference for the further use of FDG as a cattle feed resource. |
| first_indexed | 2024-03-09T17:05:25Z |
| format | Article |
| id | doaj.art-650c34e9e045427f87913c57dda35cf2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:05:25Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-650c34e9e045427f87913c57dda35cf22023-11-24T14:24:30ZengMDPI AGAnimals2076-26152023-11-011322343710.3390/ani13223437Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling CattleGuangxia He0Chao Chen1Shihui Mei2Ze Chen3Rong Zhang4Tiantian Zhang5Duhan Xu6Mingming Zhu7Xiaofen Luo8Chengrong Zeng9Bijun Zhou10Kaigong Wang11Erpeng Zhu12Zhentao Cheng13College of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaCollege of Animal Science, Guizhou University, Guiyang 550025, ChinaFermented distillers’ grains (FDG) are commonly used to enhance the health and metabolic processes of livestock and poultry by regulating the composition and activity of the intestinal microbiota. Nevertheless, there is a scarcity of research on the effects of the FDG diet on the gastrointestinal microbiota and its metabolites in cattle. This study examines the impact of FDG dietary supplements on the gastrointestinal flora and metabolic profile of Guanling cattle. Eighteen cattle were randomly assigned to three treatment groups with six replicates per group. The treatments included a basal diet (BD), a 15% concentrate replaced by FDG (15% FDG) in the basal diet, and a 30% concentrate replaced by FDG (30% FDG) in the basal diet. Each group was fed for a duration of 60 days. At the conclusion of the experimental period, three cattle were randomly chosen from each group for slaughter and the microbial community structure and metabolic mapping of their abomasal and cecal contents were analyzed, utilizing 16S rDNA sequencing and LC-MS technology, respectively. At the phylum level, there was a significant increase in Bacteroidetes in both the abomasum and cecum for the 30%FDG group (<i>p</i> < 0.05). Additionally, there was a significant reduction in potential pathogenic bacteria such as Spirochetes and Proteobacteria for both the 15%FDG and 30%FDG groups (<i>p</i> < 0.05). At the genus level, there was a significant increase (<i>p</i> < 0.05) in Ruminococcaceae_UCG-010, Prevotellaceae_UCG-001, and Ruminococcaceae_UCG-005 fiber degradation bacteria. Non-target metabolomics analysis indicated that the FDG diet significantly impacted primary bile acid biosynthesis, bile secretion, choline metabolism in cancer, and other metabolic pathways (<i>p</i> < 0.05). There is a noteworthy correlation between the diverse bacterial genera and metabolites found in the abomasal and cecal contents of Guanling cattle, as demonstrated by correlation analysis. In conclusion, our findings suggest that partially substituting FDG for conventional feed leads to beneficial effects on both the structure of the gastrointestinal microbial community and the metabolism of its contents in Guanling cattle. These findings offer a scientific point of reference for the further use of FDG as a cattle feed resource.https://www.mdpi.com/2076-2615/13/22/3437fermented distillers’ grains (FDG)gastrointestinal microbiotametabolic pathwaysGuanling cattle |
| spellingShingle | Guangxia He Chao Chen Shihui Mei Ze Chen Rong Zhang Tiantian Zhang Duhan Xu Mingming Zhu Xiaofen Luo Chengrong Zeng Bijun Zhou Kaigong Wang Erpeng Zhu Zhentao Cheng Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle Animals fermented distillers’ grains (FDG) gastrointestinal microbiota metabolic pathways Guanling cattle |
| title | Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle |
| title_full | Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle |
| title_fullStr | Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle |
| title_full_unstemmed | Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle |
| title_short | Partially Alternative Feeding with Fermented Distillers’ Grains Modulates Gastrointestinal Flora and Metabolic Profile in Guanling Cattle |
| title_sort | partially alternative feeding with fermented distillers grains modulates gastrointestinal flora and metabolic profile in guanling cattle |
| topic | fermented distillers’ grains (FDG) gastrointestinal microbiota metabolic pathways Guanling cattle |
| url | https://www.mdpi.com/2076-2615/13/22/3437 |
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